小菘菜花药培养获得再生植株

小菘菜,[Brassica campestns ssp.chinensis(L.)Makino]在日本普遍栽培近年我国引进其多个品种。作者拟通过单倍体育种途径选育小菘菜新品种来加快育种进程。以引自日本的小菘菜品种‘蕾用菜’(图版,A)为试材,种子经春化处理后穴盘育苗,盆栽。开花后选取健壮植株花序,选择小孢子发育处于单核靠边期至双核早期的花蕾(长2.5～3.5 mm),先用蒸馏水冲洗30 min,再用     

The latent gene expression of Epstein-Barr virus in nasopharyngeal carcinoma

AIM: To examine the latent membrane protein 1(LMP1)-DNA sequence in nasopharyngeal carcinoma(NPC) and detect mRNA expression of LMP1,EBNA1,EBNA2,and to explore the relationship between EBV infectious status,expression products and NPC carcinogenesis.METHODS: LMP1 DNA was detected in NPC by PCR.Direct sequence was applied to analyze the difference between NPC-LMP1-DNA and B95-8- LMP1-DNA.mRNA expressions of LMP1,EBNA1,EBNA2 in NPC were detected by nested RT-PCR.RESULTS: LMP1 DNA existed in all 47 NPC tissues.Several single nucleotide variations were found between NPC-LMP1-DNA and B95-8- LMP1-DNA.The notable variation was the lost of XhoⅠrestriction site in NPC.Direct sequence showed 30 bp deletion in NPC.The mRNA expressions of LMP1,EBNA1 and EBNA2 in NPC were 76.6%,80.0% and 74.5% respectively by nested RT-PCR.The expression of EBNA1 in NPC was promoted by Q promoter while the expression of EBNA1 in B95-8 was promoted by C promoter.CONCLUSION: The way of EBV involved in NPC is complex.Latent genes such as LMP1,EBNA1 and EBNA2 as well as early lytic gene BARF1 may all play certain roles in NPC carcinogenesis.     

Experimental study on how brain-dead state affects the heart structure and function of Ba-Ma mini pigs and the mechanism

AIM:To investigate how brain-dead state affects the heart structure and function and the effect of PKC-α in BA-Ma mini pigs.METHODS:Ten Ba-Ma mini pigs were randomized into 2 groups: brain-dead group (n=5),and control group (n=5). The brain-dead model was made by increasing intracranial pressure,while the control group was maintained anesthesia for 24 h. The concentrations of cTnT,TNF-α,IL-1β and IL-6 in serum were determined at 6,12 and 24 h after brain death. At 24 h,heart tissues were observed by HE staining and electron microscope. The expression of PKC-α was detected by immunohistochemistry and RT-PCR.RESULTS:(1) Histological changes of myocardium: flaky bleeding under endocardium and dissolution of myocardium were found in optical microscope. In electron microscope dropsical mitochondria and confluent muscle fiber were found. (2) Changes of serum cTnT: serum cTnT for brain-dead group began to increase gradually since 6 h,and were significantly higher at each time point than those in control group (P<0.05). (3) Changes of inflammatory factors: IL-1β,IL-6,and TNF-α in brain-dead group began to increase gradually since 6 h,and were significantly higher at each time point than those in control group (P<0.05). (4) Changes of PKC-α expression: PKC-α mRNA and protein expressions in brain-dead group increased significantly at 24 h (P<0.05).CONCLUSION:Brain death may evoke heart structure and functional injury,and increase the levels of inflammatory factors and PKC-α. The activation of PKC-α may participate in the process of heart injury.     

苏云金杆菌HBF-1菌株在土壤中毒蛋白含量的ELISA检测

以苏云金芽孢杆菌HBF-1菌株中分离纯化的高特异性杀虫蛋白作为抗原，免疫新西兰白兔制备出多克隆抗体，其效价可达到16000。应用制备的特异性抗体建立了间接酶联免疫吸附测定法（ELISA），检测不同土壤样品中Bt毒蛋白含量。初步结果表明，该方法能够检测土壤中Bt毒蛋白的含量，因土壤理化特性及组成成分的不同，检测到的毒蛋白含量也略有不同，河沙中的蛋白检测量最低，只有12．53％；另一种沙壤混合物中检测量达到76.16％。     

拉萨市林周县牦牛乳样理化指标及乳房炎病原菌的分离鉴定

为研究西藏拉萨市林周县牦牛乳理化指标和乳房炎主要病原菌,采集牦牛乳样品100份,开展乳理化指标和主要病原菌检测。采用CMT法筛选隐性乳房炎病例,针对筛选出隐性乳房炎阳性乳样20份进行病原菌分离鉴定,共分离出11种52株细菌,通过鉴定,其中包含4株凝固酶阴性葡萄球菌,10株金黄色葡萄球菌,2株停乳链球菌,2株乳房链球菌,1株粪链球菌,8株无乳链球菌,6株大肠埃希菌,4株真菌。乳房炎63.5%的病例是由链球菌、葡萄球菌和大肠埃希菌引起,结果证实,链球菌、葡萄球菌和大肠埃希菌是导致林周县牦牛乳房炎的主要病原菌。     

鹅源H5N1亚型禽流感病毒感染SPF雏鸡免疫球蛋白含量的变化研究

以7日龄SPF雏鸡为试验动物,采用常规间接酶联免疫吸附试验(IELISA)法,对鹅源H5N1亚型禽流感病毒(AIV)感染SPF雏鸡后,其泪液、气管液、肠液、胆汁的IgA、IgG、IgM含量的动态变化进行了检测.结果发现,鹅源H5N1亚型中强毒AIV感染SPF雏鸡后,其泪液、气管液、肠液、胆汁的免疫球蛋白IgA、IgM含量均在1～3 d或1～4 d显著低于相应对照雏鸡(P＜0.05),而感染后第5天,上述被检指标开始升高,并于5～8 d或5～14 d,病毒感染雏鸡的IgA和IgM显著高于对照雏鸡(P＜0.05或P＜0.01).表明7日龄SPF雏鸡感染AIV后的早期,其消化道和呼吸道的体液免疫功能降低.     

MrMYB1-MrbHLH1:一个有潜力的园艺植物转基因可视化报告基因

将杨梅果实中参与花色苷生物合成的转录因子编码基因MrMYB1和MrbHLH1同时搭载到双元表达载体的多克隆位点内,重组成双价表达载体,通过农杆菌介导的叶盘法将其分别转入烟草、番茄和森林草莓,成功转化MrMYB1-MrbHLH1的烟草、番茄和森林草莓不定芽因大量积累花色苷而呈红色,与野生型或假阳性不定芽相比,表型差异明显;转化35S::MrMYB1-MrbHLH1的烟草和番茄植株中可显著积累花色苷呈红色,且该性状可稳定遗传。MrMYB1-MrbHLH1在3种园艺植物具有调控花色苷积累而使阳性转化植物部分组织器官呈现红色的功能,是1个有潜力的遗传转化可视化报告基因。     

Comparison of Blood Physiological and Biochemical Parameters of Two Kinds of Minipigs in Different Altitudes

This study was designed to compare the blood physiological and biochemical parameters between plateau Juema minipigs and plain Bama minipigs,and provide a theoretical basis for Juema minipigs served as experimental animals in the future.The blood samples of Juema minipigs were taken in Gannan,and the blood samples of the Bama minipigs were taken in Xi'an,24 blood physiological and biochemical parameters were tested using an automatic analyzer of blood cells and biochemistry.Between the plateau Juema minipigs and Bama minipigs,there were significant differences in PLT,RDW-CV and RDW-SD (P< 0.05) and extremely significant differences in RBC,HGB,HCT,MCH,MCMH,GGT,GLU and CRE (P< 0.01).The plateau Juema minipig had an altitude acclimatization,so it was better than Bama minipig in plateau medical science.