条件性敲除D1133L基因的重组非洲猪瘟病毒的构建及增殖特性

前期研究初步表明非洲猪瘟病毒(ASFV)编码的D1133L基因对ASFV复制至关重要,本研究拟进一步探究D1133L在ASFV复制中的作用。利用同源重组技术结合大肠杆菌lac阻遏操作系统实现条件性敲除D1133L基因,以pUC118为骨架重组转移载体ASFVΔi130,将重组转移载体转染骨髓源巨噬细胞(BMDMs),以ASFV CN/GS/2018为亲本毒株感染BMDM,在β-D-硫代半乳糖苷(IPTG)存在的条件下,经绿色荧光和PCR鉴定,获得条件性敲除D1133L重组毒株vD1133Li。利用荧光显微镜观察该重组毒株与亲本毒株在猪肺泡巨噬细胞(PAMs)中的复制差异,利用qPCR技术比较重组病毒与亲本毒株的复制差异,分析vD1133Li在无IPTG情况下回补D1133L蛋白后与在IPTG诱导情况下的复制差异。结果显示：本研究成功构建了条件性敲除D1133L的ASFV重组病毒vD1133Li,重组毒株不表达D1133L,在IPTG诱导下复制能力显著低于亲本毒株;在稳定表达D1133L的MA-104细胞系中,vD1133Li复制能力恢复。综上所述,D1133L基因对于ASFV复制至关重...     

建立发扬中国传统文化的管理模式

本文论述了中西方文化对于管理的影响,分析了中国式管理出现的文化因素,总结了中国传统文化在现代管理中可加以运用的结合点,提出应该更有自信的看待我们自己管理方式的观点。     

System of Scanning Tunneling Microscope

Some ideas are given about using out-clock, CPU and anti-T-net of D/A card for getting to the intelligent control of scanning voltage ,low-filter and hign-filter of STM; logarithm amplifier for tunnelling current contraction. At last, some applications and questions when it running about STM are discussed.     

Digital Beamforming for Unified Spread Spectrum TT&C System

In TT&C system,unified spread spectrum framework and adaptive antenna digital beamforming technologies are used to track, telemetry and command objects. Constant modulus array (CMA) and adaptive signal canceller estimate the direction of arrival (DOA) of every signal and use the optimum weighted vector to form the beams. The phase weighted vectors estimated from DOA improve the processing gain as well as being utilized in the transmitting of tele command signal to form the beam of specified object. Its processing performance is researched and simulated in this paper.     

Research on the System and Models of Society-Economy-Environment Appraisal for Construction Project

The society-economy-environment appraisal for construction project can help our government to control macro-economy, make correlation policy and formulate laws and draw up city plan.It has great influence on the sustainable development of our society and it is the demand of the law for the environment evaluation in China.This paper introduced the basic structure of society-economy-environment appraisal system for construction project and the thought of elements transaction.Using the method of AHP, the model of project analyzing and optimizing selection is designed.For the more, the methods of the optimizing models on the selected schemes are put forward.Finally, the integrative analysis has been made on the system design and appliance of this appraisal system.     

Power Frequency Electric Field Caused by EHV Lines and Its Effect

Mathematical model of power frequency electric field is established based on Charge Simulation Method(CSM) to analyze power frequency electric field caused by EHV lines with overhead ground wires and its effect, which are entering the megalopolis. Calculative method on the electric field under the transmission lines is analyzed and validated. According to the criterion of environmental evaluation, the distributing characteristic of electric field is summarized, in case of various arrangement manners of lines. At last, some advice and methods of eliminating electric field under the transmission lines entering the megalopolis is proposed with regard to protecting environment.     

An Estimate on the Casual Relation between Financial Development and Economic Growth

This paper firstly discusses the relationship among money,real interest rate and economic growth of China. Then estimates the causality between financial development and economic growth by Bivariate Granger Casual Relation Test. The conclusion suggests that economic growth stimulate financial development more in China.     

Sampling Investigation on Inhatitant Domestic Tap Water Consumption Situation and It’s Characteristics Analysis

Economics.Through sampling investigation and analysis on inhabitant domestic tap water consumption situation,it is given that economic factors are the main factors influence inhatitant's water consumption.The functional relations among inhabitant's income and water consumption,water price and consumption are given. The tap water's income elasticity of demand and price elasticity of demand is analyzed, along with the fluctuating range month by month of inhabitant's water consumption.Finally ,this paper offers the reference data for planning the ability of water supply and making policies of ladder water prices.     

Robust Adaptive Control Based on On-line Identification to Multi-parameters

In system controlled,Sampling these parameters and with parameters for controller in time, it is an object to adaptive control. The article presents a scheme that the structure parameters on it's model and model parameters is robust identified by on line, while to optimal of algorithm on adaptive control is fulfilled. In control scheme, method of intelligent identifying to the structure parameters(levels of model) and algorithm of robust pole placement are introduced also It is advancer for already mentioned scheme. Whole scheme presents new model on adaptive control.     

Microstructures, Reaction Kinetics and Properties of Reaction Between Magnesium and Silica Glass

In earlier calculation models,the stiffness centers in frame-shear wall structures have been located by the structural torsion stiffness.However,the results are illogical owing to the difference between the distortion character and the shears distribute mechanism of the frames and shear walls.In this paper,a new formula for calculating story stiffness is introduced,which can consider the stiffness of the frames and shear walls at the same time.The structural stiffness centers,the torsion eccentric distances and torsion effect are calculated,and the stiffness of elements is considered also.It is shown that the concept of the presented stiffness model is clear and the results are reasonable,which can be used to calculate the appreciative torsion effect.