Isolation and Identification of Pathogenic Bacteria Causing Dairy Cow Mastitis in Liaoning and Drug Sensibility Test of Pathogenic E.coli

In order to understand the main bacterial pathogen species causing dairy cow mastitis in Liaoning, as well as the characteristics of drug sensitivity of the pathogenic E.coli,the milk samples from 75 dairy cows with clinical manifestations for mastitis in certain large-scale dairy farm in Liaoning were collected.The bacteria in milk were cultured and isolated with biochemical methods and in vitro drug sensitivity tests were processed with the isolated E.coli strains.The results showed that the main bacterial pathogen for dairy cow mastitis were E.coli（separation rate 58.7%),S.aureus（64.0%）and S.agalactiae（54.7%),and multiple infection including double and triple infection were identified.The drug sensitivity tests on the isolated E.coli indicated that the E.coli isolates were highly resistant to sulfonamides（resistance rate>85%）and chloramphenicol（resistance rate>30%),and they were relatively low resistant to ampicillin（9.5%),ciprofloxacin（9.5%),ceftiofur（7.1%）and ofloxacin（4.8%).The results was able to provide reliable theoretical basis for prevention and control of dairy cow mastitis in Liaoning area.     

贮藏温度对采后“金艳”猕猴桃品质和后熟的影响

以"金艳"猕猴桃为试材,采用不同温度贮藏的方法,研究了温度对果实品质和呼吸强度的影响。结果表明:1℃贮藏可以很好的保持果实品质,呼吸强度峰值出现晚,保持了较高的果肉硬度;5℃和10℃贮藏能够促进果实的后熟,加速呼吸强度峰值的到来、果实硬度的下降和可溶性固形物含量的上升;但35℃贮藏的果实7d后会失去商品价值,发生腐败;16℃贮藏果实后熟品质不佳。在整个贮藏过程中,果实干物质含量在上述温度条件下贮藏变化都不明显,但在一定范围内,干物质含量会随着温度的上升而有所增加。     

“赤霞珠”葡萄组培快繁体系研究

以"赤霞珠"葡萄为试材,对组培快繁中的取材部位、消毒方法、不同激素用量、是否带叶继代培养等关键技术措施进行了研究探讨。结果表明:以半木质化,0.1%升汞溶液消毒8min的茎段接种,成活率高,污染率低;并且继代培养和生根培养最好的培养基为1/2MS+IBA0.2mg·L~(-1)+蔗糖20g·L~(-1)+琼脂7g·L~(-1)。     

水分胁迫对幼龄仁用杏叶片光合特性的影响

以2年生仁用杏为试材,采用盆栽方法,以正常供水(土壤含水量为田间最大持水量的75%)为对照,设置了轻度水分胁迫(55%)、中度水分胁迫(45%)和重度水分胁迫(30%)3个处理,开展了水分胁迫条件下仁用杏叶片光合色素、光合指标及叶绿素荧光参数等光合特性研究。结果表明:水分胁迫不同程度降低了类胡萝卜素含量、叶绿素a、b含量、净光合速率(Pn)、蒸腾速率(Tr)、水分利用效率(WUE)、最大荧光产量(Fm)、最大光化学效率(Fv/Fm)和PSⅡ潜在活性,且下降幅度随着胁迫时间延长而增大;轻度水分胁迫下降幅度很小,中度和重度水分胁迫下降幅度较大。     

珍稀濒危植物膝柄木种子催芽研究

以采自越南广宁省海河县的膝柄木种子为试材,研究了不同药剂和浓度对种子发芽率和芽苗主根生长的影响。结果表明:与水浸相比,艾比蒂生根剂1号和双吉尔氨基酸肥料6号0.5~15.0mg/L浸种1h,对膝柄木种子发芽均没有促进作用,可见水浸即可有效促进膝柄木种子发芽;艾比蒂生根剂1号0.5mg/L和双吉尔氨基酸肥料6号0.5~10.0mg/L对膝柄木芽苗主根生长有很好的促进作用。     

野生老芒麦种质资源鉴定与基部叶鞘绒毛变异分析

为更好地利用老芒麦(Elymus sibiricus L.)野生资源,本研究从我国青藏高原、西北、华北、东北地区以及国外部分地区共采集了1 723份野生披碱草属种质资源,通过表型特征观测和流式细胞仪检测,共鉴定出了990份野生老芒麦种质,246份垂穗披碱草种质,并测得老芒麦的DNA含量在5.86～7.30 Gb之间,平均DNA含量为6.66 Gb;垂穗披碱草材料的DNA含量在9.50～10.36 Gb之间,平均DNA含量为9.97 Gb。研究结果显示,在西北、华北以及东北地区的部分野生老芒麦材料在苗期基部叶鞘出现了绒毛,分析发现该表型出现的概率与经度和纬度呈极显著正相关关系(P<0.01),与海拔、年年均气温以及年平均降雨量呈极显著负相关关系(P<0.01)。表明老芒麦苗期基部叶鞘绒毛的有无与环境因子之间存在着必然的联系,可能是老芒麦种质应对不同环境类型的适应性机制之一。本研究为老芒麦的形态鉴定提供了新的依据,为老芒麦种质资源挖掘和育种应用提供了材料基础。     

建立发扬中国传统文化的管理模式

本文论述了中西方文化对于管理的影响,分析了中国式管理出现的文化因素,总结了中国传统文化在现代管理中可加以运用的结合点,提出应该更有自信的看待我们自己管理方式的观点。     

Digital Beamforming for Unified Spread Spectrum TT&C System

In TT&C system,unified spread spectrum framework and adaptive antenna digital beamforming technologies are used to track, telemetry and command objects. Constant modulus array (CMA) and adaptive signal canceller estimate the direction of arrival (DOA) of every signal and use the optimum weighted vector to form the beams. The phase weighted vectors estimated from DOA improve the processing gain as well as being utilized in the transmitting of tele command signal to form the beam of specified object. Its processing performance is researched and simulated in this paper.     

Wavelet Edge Detection Method Based-on Multi-scale Fuzzy Logic

A novel edge detection algorithm is proposed by implementing fuzzy image enhancement at different scales.The implement of this algorithm can reduce the negative influence bring from the non-maximum suppression algorithm which select only one threshold.The success of the approach depends on the selection of a general fuzzy operator(GFO) for the enhancement of contrast of region after smoothing of image at different scales.Consequently,it is more obvious that coarse intensity changes are obtained at a large scale whereas the fine details of intensity changes are obtained at a small scale.A pretty performance is obtained by used this work.