2004—2013年我国空间格局分析的方法-点格局分析法研究文献分析

为了解我国近十年来点格局分析法方面的研究现状,采用文献计量学等统计方法,对2004—2013年我国空间格局分析的方法-点格局分析法研究方面的文献进行了分类统计分析。结果共检索出点格局分析法方面的文献232篇。其中不同期刊的载文分布,发文量达10篇以上的期刊有5种,占32.33%。不同年份发文量,2004年发表的文献量最少,仅3篇;2012年最多,达到41篇。不同第一作者发文量,发文量少于5篇的作者最多,占59.48%;发文量为5篇的有9人,占19.40%;发文量为6~9篇的有5人,占16.38%;发文量为10篇或以上的仅1人,占4.74%。作者机构分布,在所搜集的232篇文献中,有9个机构在此期间发表的论文7篇,占71.55%。文献的研究层次,基础与应用基础研究的发文量最多,占93.10%。     

ELISA鉴别条件下的野生羊肚菌菌种分离方法

为了提高分离的羊肚菌菌种的可靠性,在传统菌种分离的基础上,增加了酶联免疫吸附试验(ELISA)鉴别检验。对采自于吉林省吉林市的野生羊肚菌发生地的羊肚菌土壤基质及子实体分别进行了菌种分离,对分离得到的3株疑似羊肚菌的菌株进行了间接酶联免疫吸附法(间接ELISA)免疫学方法检测,确定其中的2株纯培养菌丝为羊肚菌菌种。     

Effects of maternal limb ischemic preconditioning on structural and functional changes of mitochondria in fetal hippocampal neurons induced by intrauterine distress-reoxygenation in rats

AIM: To investigate the effects of maternal limb ischemic preconditioning (LIP) on the mitochondrial structures and functions of the hippocampal neurons induced by reoxygenation in the intrauterine distress fetal rats. METHODS: Pregnant rats (n=40) were randomly divided into 4 groups: sham (S) group, LIP group, fetal distress (FD) group and LIP+FD group. Intrauterine ischemia model was established through the experimental design. The ultrastructure of the mitochondria in CA1 area of the hippocampus was observed. The mitochondrial membrane potential and reactive oxygen species (ROS) were measured. The content of ATP and MDA in the hippocampus tissue was detected. The activity of Mn-SOD was observed. RESULTS: Compared with sham group, the ultrastructure of mitochondria in CA1 area of the hippocampus was damaged in FD group and LIP+FD group. The mitochondrial membrane potential, the content of ATP and the activity of Mn-SOD were decreased. However, the content of ROS and MDA was increased. Compared with FD group, the ultrastructure of mitochondria in CA1 area of the hippocampus was intact in LIP+FD group. Furthermore, the reduced mitochondrial membrane potential and ATP content were inhibited. The activity of Mn-SOD was increased, but the content of ROS and MDA was decreased in LIP+FD group. CONCLUSION: Limb ischemia preconditioning inhibits the damage the mitochondria of fetal hippocampal neurons induced by reoxygenation in the intrauterine distress fetal rats.     

蟠桃新品种‘中蟠桃11号’

‘中蟠桃11号’是以‘红珊瑚’ב91-4-18’(NJN78×奉化蟠桃)杂交,结合胚挽救技术获得的蟠桃新品种。花铃形。单果质量180~200 g,果皮60%以上着鲜红色,果肉橙黄色,肉质为硬溶质。果实风味浓甜,可溶性固形物含量14%,粘核。耐运输。7月中下旬成熟,果实发育期120 d。极丰产,平均产量为38 888 kg·hm-2,     

甘蓝杂种致死材料的鉴定与遗传分析

杂种致死是一种生殖隔离类型,致死材料的鉴定及遗传分析不仅有助于了解杂种致死的遗传机制,还可以为致死基因的定位和克隆奠定基础。利用已知携带杂种致死基因的甘蓝自交系09-211、09-222、10-260和其他自交系配制的杂交组合为试材,鉴定携带致死基因的新材料,并对甘蓝杂种致死性状进行了遗传分析。结果表明:11-204和11-176是2个新的甘蓝致死材料;11-176与09-222及10-260具有遗传等效性,而11-204与09-211具有遗传等效性;甘蓝杂种致死性状是由2个显性基因互补控制的,其中1个致死基因来自09-211或11-204,另1个致死基因来自09-222或10-260或11-176,其遗传模式符合Dobzhansky-Muller(DM)模式。     

Hsa-miR-218 inhibits growth of cervical cancer HeLa cells by targeting to LASP1

AIM: To study the effect of hsa-miR-218 on cervical cancer HeLa cell growth and the underlying molecular mechanism.METHODS: The lentivirus expression vector pmiR-218 targeting to hsa-miR-218 was constructed. pmiR-218 was transfected into HeLa cells. The number of viable HeLa cells was counted by the method of Trypan blue exclusion. The inhibitory rate of cell activity was detected by WST-8 assay. The expression of LIM and SH3 protein 1(LASP1) at mRNA and protein levels was determined by real-time PCR and Western blot. The interaction between miR-218 and LASP1 was examined using a luciferase reporter assay.RESULTS: The lentivirus expression vector pmiR-218 targeting to hsa-miR-218 was constructed successfully and confirmed by DNA sequencing. Over-expression of miR-218 inhibited the activity of HeLa cells with the inhibitory rates of 15%, 26% and 65% at 24 h, 48 h and 72 h, respectively. The difference between transfection group and blank control/negative control group was statistically significant. The luciferase activity was reduced when co-transfection with miR-218 mimics and LASP1-3,UTR plasmid. The relative expression of miR-218 was increased after transfection with pmiR-218. Over-expression of miR-218 down-regulated the LASP1 expression at mRNA and protein levels by 25% and 75% respectively. Compared with blank control group and negative control group, the difference was statistically significant(P<0.05).CONCLUSION: pmiR-218 effectively inhibits the growth of HeLa cells in a time-dependent manner. miR-218 targets to the 3,UTR of LASP1, thus down-regulating the expression of LASP1 in HeLa cells.     

过氧化氢诱导不同色素水平葡萄愈伤组织白藜芦醇及白藜芦醇苷含量差异

以不同状态的"红地球"葡萄愈伤组织为试材,采用高效液相色谱法,研究H2O2对其白藜芦醇和白藜芦醇苷含量的影响。结果表明:经H2O2诱导后3种愈伤组织中白藜芦醇苷含量为:红色愈伤组织褐化的愈伤组织白色愈伤组织,而白藜芦醇在白色愈伤组织中的含量高于红色和褐化的愈伤组织。2.5μM H2O2诱导红色愈伤组织24h后,白藜芦醇苷含量高达306μg/gFW,而白藜芦醇的含量变化不明显。红色愈伤组织中的白藜芦醇苷响应H2O2的胁迫较为明显,其对H2O2最佳的响应浓度为2.5μM。     

大豆叶片性状和叶绿素含量QTL间的上位性和环境互作效应

以丰产性好、抗旱力强的栽培大豆晋豆23为母本,山西农家品种半野生大豆灰布支黑豆为父本杂交衍生的447个RIL作为供试群体。将亲本及447个家系分别于2011、2012和2013年采用随机试验种植,按照标准测量叶长、叶宽和叶柄长3个性状,并于2012年8月1日和8月8日和2013年8月2日和8月9日各测量1次叶绿素含量。采用QTLNETwork 2.0混合线性模型分析方法和主基因+多基因混合遗传分离分析法,对大豆叶片性状和叶绿素含量进行遗传分析和QTL间的上位性和环境互作效应研究。结果表明,叶长受2对加性-加性×加性上位性混合主基因控制,叶宽受3对等效主基因控制,叶柄长受4对加性-加性×加性上位性主基因控制,叶绿素含量受4对加性主基因控制;检测到10个与叶长、叶宽、叶柄长和叶绿素含量相关的QTL,分别位于A1、A2、C2、H_1、L和O染色体。其中2个叶长QTL分别位于C2和L染色体,是2对加性×加性上位互作效应及环境互作效应QTL;3个叶宽加性与环境互作QTL分别位于A2、C2和O染色体;2个叶柄长QTL分别位于L和O染色体;3个叶绿素含量QTL分别位于A1、C2和H_1染色体。叶片性状和叶绿素含量的遗传机制较复杂,加性效应、加性×加性上位互作效应及环境互作效应是大豆叶片性状和叶绿素含量的重要遗传基础。建议大豆分子标记辅助育种中,一方面要考虑起主要作用的QTL,另一方面要注重上位性QTL的影响,这对于性状的遗传和稳定表达具有积极的意义。     

基于Glb 1基因序列的西南地区玉米地方品种的系统进化

为探讨我国西南地区玉米地方品种Glb 1基因序列多态性,对40份西南地区玉米地方品种的Glb 1基因序列测序,并与Gen Bank中来自普通玉米、小颖玉米、繁茂玉米和摩擦禾的19条同源序列比对。结果表明,来自西南地区四川、重庆、云南和贵州的玉米地方品种分别有35、25、33和25个多态性位点,其遗传多态性分别是小颖玉米的87%、86%、85%和71%,8个种群遗传多态性指数的大小排序为小颖玉米、四川地方品种、重庆地方品种、云南地方品种、贵州地方品种、普通玉米、磨擦禾和繁茂玉米,Tajima’D、FuLi’D和FuLi,F检验表明Glb 1基因是中性进化基因。比较种群间DNA序列的共有多态性和固定差异,玉米地方品种与小颖玉米、繁茂玉米、磨擦禾间存在较高的固定差异和较低的共有多态性。Glb 1基因的系统进化关系及网状关系分析为玉蜀黍属的分类系统提供了佐证,四川玉米地方品种有较高的遗传多态性支持我国玉米由印度经西藏传入四川的论点。     

鳞翅目蛾类卵形态的比较研究

利用显微观察并描述了鳞翅目亚麻篱灯蛾、驼波尺蛾、榆绿天蛾、雪毒蛾、灰双纹螟、尖锥额野螟、银锭夜蛾、荒夜蛾卵壳的形态特征。结果表明，不同种类蛾卵受精孔的数目；受精孔区花饰的形状、数目、排列方式；气孔的位置与排列均存在明显的差异，这些差异为卵的分类提供了依据。