温光和种植制度对棉花早熟性和纤维品质的影响

研究了气候和种植制度对棉花早熟性和主要品质的影响,结果表明:棉花早熟性和纤维长细强指标受温光影响最大,熟制次之,栽培技术第三。进一步分析,气温中日最低温度对纤维品质影响最大。温光、熟制和栽培技术有互作效应,三者互作对细度影响最大,强度次之,长度影响最小;采用促早栽培,提高霜前花率有利于改进整体纤维品质。     

LM-BP神经网络水质评价模型应用研究——以新乡市卫河为例

介绍了LM-BP神经网络模型的原理及算法和模型的优点。针对实际水质评价问题,利用随机内插方法在地表水环境质量分级标准阈值间生成训练样本和检验样本,建立了新乡市卫河地面水环境质量综合评价的LM-BP神经网络模型,将模型应用于卫河2011年3月份、9月份的水质评价,并与单因子评价法、模糊综合评价法进行了比较分析。实验结果表明该模型设计合理,泛化能力强,收敛速度快,算法稳定,推导严谨,有较充分的理论依据,应用于水质评价具有其合理性、实用性和有效性,适用于作深入的水环境质量分析。     

呼伦贝尔地区不同土地利用下土壤蒸发野外试验研究

土壤蒸发是水文过程的关键环节之一,控制着水分由土壤环境向大气环境的扩散。通过在呼伦贝尔地区典型草地与林地设置相同口径不同深度微型蒸发仪(Micro-Lysimeter,简称ML)的对比,寻求适合林地草地使用的微型蒸发仪,并研究不同土地利用方式对土壤蒸发的影响,探讨环境因素对土壤蒸发结果的影响。结果表明:土壤蒸发量随ML长度的增加先增大后减小,相同直径长度不同ML的蒸发量在0.05水平下显著相关,测定林地的蒸发量小于草地蒸发量,不封底ML所测定结果比封底ML大,其结果更接近真实情况,测量呼伦贝尔地区土壤蒸发时,亦采用15cm的不封底ML,结果较好。     

土壤入渗传输函数输入变量分析

依据土壤水分入渗参数与其相关土壤理化参数之间的相关性,从土壤学的角度用物理的方法分析应县土壤常规土壤理化参数对土壤入渗传输函数影响程度,进一步确定其土壤入渗传输函数的输入变量。并用大田耕作土壤水分入渗参数及其土壤理化参数的实测资料,建立土壤入渗传输函数进行验证。     

SAR,EC与水温对辽西褐土入渗速率的影响研究

土壤入渗速率是影响灌溉水产流、侵蚀的重要因素之一。为了研究不同水温、不同SAR/EC下辽西褐土入渗速率,进行了室内变水头渗透试验。结果表明:SAR,EC一定时,渗透系数随着灌溉水水温的升高而增大,水温从15℃升至35℃,土壤渗透系数增大了1.3倍;随着灌溉水SAR增加、EC降低,土壤渗透系数会下降,水温35℃情况下SAR从0.7(mmol/L)0.5增大至12.0(mmol/L)0.5,EC从2.0dS/m减小至0.5dS/m时,土壤渗透系数由0.000 698cm/s减小至0.000 338cm/s,渗透系数下降了51.6%。相关分析表明水温、SAR/EC对渗透系数的影响显著,回归分析分别建立了沟灌土壤渗透系数与水温、SAR/EC二次函数数学模型,相关性极好。     

Effect of Weaning Stress on Blood Physiology and Biochemistry Indexes and Immune Function in Dairy Buffalo Calves

To study the effect of weaning stress on dairy buffalo calves,5 healthy calves were chosen and weaned,and the blood samples were collected at 1 day before weaning,and 1,14 and 21 days after weaning to analysis blood physiology and biochemistry indexes and immune function.The results showed that the count of white blood cell,lymphocyte,erythrocyte,monocytes and hematocrit,aspartate aminotransferase activity,blood glucose,cortisol and immunoglobulin G contents of dairy buffalo calves were no significant difference between before and after weaning treatments (P > 0.05).The count of neutrophils at 21 d after weaning were significantly lower than that of 1 d before weaning of dairy buffalo calves (P < 0.05).However,lactate dehydrogenase and alanine aminotransferase activity in weaned treatments were significantly higher than those of animals in the treatment before weaning (P < 0.05).The serum urea nitrogen of dairy buffalo calves at 21 d after weaning was significantly higher than that of 1 d before weaning (P < 0.05) and hemoglobin content at 21 d after weaning was significantly lower than that at 1 day before weaning (P < 0.05).It indicated that the physiological and immunity function of dairy buffalo calves were challenged under weaning stress.     

Effects of lncRNA-GTL2 Transgenosis on Gut Microbiota Composition in Mice

This research was aimed to study whether the lncRNA would have effects on the structure and constitution of the intestinal microbial colonies in mice.High throughout sequencing was used to sequence and analyze the intestinal microbial colonies in both transgenic and non-transgenic mice of three months old.We conducted comparison and t-test at the level of phylum and genus.The result showed that transferred into long non-coding RNA genes GTL2 (lncRNA-GTL2),the mouse intestinal microbial colony structure and composition had no significant differences in the overall,within the same gender,but there were individual differences.Therefore,this study didn't find that long non-coding RNA transfered had a significant impact on the intestinal microflorain the phylum and genus level.     

Effects of Donor Cells Treated with Egg Extracts on the Development Competence of Cloned Embryos

Japanese Balck cattle fetal fibroblasts (JBCFF) were induced with Xenopus leavis egg extracts and somatic cell nuclear transfer (SCNT) was carried out with the reprogrammed JBCFF as donor cells in order to investigate their effects on SCNT efficiency.Three samples of egg extracts were acquired from different Xenopus laevis.The protein contents and kinds in extracts were evaluated with BCA Protein Quantification Kit and SDS-PAGE.Concentration of Digitonin to permeabilize JBCFF was optimized and assessed with PI staining.Reprogrammed cells treated with egg extract were used as donor in SCNT.Additionally the reconstructed embryos were activated with ionomycin+6-DMAP and A23187+6-DMAP to compare their effects on the development competence.The protein contents of extracts samples were 56.2255,64.6570 and 71.2158 μg/mL,respectively,the each extract had the same composition about 40-55 and 70-100 ku.The optimal concentration of Digitonin was 7 μg/mL and the permeabilization rate was 55.44%.After extracts treatment and continuous culture for 6-7 d,JBCFF formed well-defined colony structures.No significant composition difference was found in rates of fusion (92.83% vs 96.04%),cleavage (89.64% vs 89.78%) and blastocyst formation (24.06% vs 23.12%) of cloned embryos when the colony cells and JBCFF without extracts treatment were used as donor cells (P>0.05).Similarly,the two activation methods had no significant effect on the developmental competence of cloned embryos (cleavage rate 92.16% vs 92.28%,blastocyst rate 23.21% vs 24.18%).Conclusively,Xenopus leavis,egg extracts could induce JBCFF reprogramming to a low differentiated state.However donor cells with reprogramming partially could not improve the development of cloned embryos and its mechanism requires further research.     

武汉地区梨瘿蚊生物学特性及其种群动态变化研究

以梨瘿蚊为研究对象,采用显微观察及田间统计方法,调查了梨瘿蚊的危害症状,梨瘿蚊卵、幼虫、成虫的形态特征及其产卵习性等生物学特性,系统研究了梨瘿蚊成虫种群和幼虫种群动态变化及梨园天敌昆虫的动态变化。结果表明:梨瘿蚊在湖北省梨产区1年发生3代,越冬代成虫羽化出土时间周期较长,从2月下旬出土,一直持续到3月下旬羽化达到高峰,梨瘿蚊幼虫种群在1年中出现3次危害高峰,危害高峰在梨瘿蚊成虫羽化后20~30d后出现;当环境温度高于30℃时,梨瘿蚊幼虫结茧化蛹,不再羽化;梨园中梨瘿蚊的天敌昆虫的种类主要包括草蛉、异色瓢虫、龟纹瓢虫、食蚜蝇、蜘蛛和蚂蚁等。     

罗布泊钾盐矿区绿化施工与管护技术

罗布泊钾盐矿区绿化工程项目区位于素有"绿化禁区"之称的罗布泊腹地,该工程克服了极端干旱、高温、多风大风、盐土和盐尘等诸多不利环境因素,采用了适宜的盐土改良措施和管护技术,让"死亡之海"出现了人工绿色,同时改善了矿区的办公环境,为极端环境下的绿化工程提供了成功案例。