Availability of Micro-Tom mutant library combined with TILLING in molecular breeding of tomato fruit shelf-life

Novel mutant alleles of an ethylene receptor Solanum lycopersicum ETHYLENE RESPONSE1 (SlETR1) gene, Sletr1-1 and Sletr1-2, were isolated from the Micro-Tom mutant library by TILLING in our previous study. They displayed different levels of impaired fruit ripening phenotype, suggesting that these alleles could be a valuable breeding material for improving shelf life of tomato fruit. To conduct practical use of the Sletr1 alleles in tomato breeding, genetic complementation analysis by transformation of genes carrying each allele is required. In this study, we generated and characterized transgenic lines over-expressing Sletr1-1 and Sletr1-2. All transgenic lines displayed ethylene insensitive phenotype and ripening inhibition, indicating that Sletr1-1 and Sletr1-2 associate with the ethylene insensitive phenotype. The level of ethylene sensitivity in the seedling was different between Sletr1-1 and Sletr1-2 transgenic lines, whereas no apparent difference was observed in fruit ripening phenotype. These results suggested that it is difficult to fine-tune the extent of ripening by transgenic approach even if the weaker allele (Sletr1-2) was used. Our present and previous studies indicate that the Micro-Tom mutant library combined with TILLING could be an efficient tool for exploring genetic variations of important agronomic traits in tomato breeding.     

Health assessment of free-ranging and captive Matschie's tree kangaroos (Dendrolagus matschiei) in Papua New Guinea

Medical evaluations were performed on free-ranging and captive Matschie's tree kangaroos (Dendrolagus matschiei) in Papua New Guinea. The health assessment included physical examination, morphometrics, cloacal swab; and blood, hair, and feces collection. Radio-collars were placed on free-ranging tree kangaroos to determine home range and forest habitat use. The free-ranging tree kangaroos were lightly anesthetized with tiletamine/zolazepam for the data collection. A total of nine free-ranging and seven captive tree kangaroos were evaluated; medical samples were collected from six and five animals, respectively. Results of physical examination, anesthetic monitoring, serum vitamin, mineral, trace nutrient, and electrolytes, whole blood heavy metal analysis, mycobacterial screening, and fecal examinations are presented. Free-ranging tree kangaroos had significantly lower values for beta carotene, copper, selenium, molybdenum, lead, and arsenic and significantly higher values for vitamin E than captive individuals. Cloacal swabs were all negative for Mycobacterium avium via polymerase chain reaction. Some free-ranging and captive individuals had positive coprologic exams revealing Eimeria spp. oocysts and strongyle spp. type ova. These are the first medical and anesthetic data published on Matschie's tree kangaroos from Papua New Guinea.     

Merkwürdige Beschädigung eines Telefonkabels durch einen Vogel

Ohne Zusammenfassung Mit 3 Abbildungen.     

Feeding Redberry Juniper (Juniperus pinchotii) at Weaning Increases Juniper Consumption by Goats on Pasture

Redberry (Juniperus pinchotii Sudw.) and ashe (Juniperus ashei Buchh.) juniper dominate rangelands throughout central Texas. Our objective was to attempt to improve the efficacy of goats as a biological control mechanism for juniper through behavioral training. Conditioning sheep and goats to increase the palatability of chemically defended plants can be a useful tool in brush control. Previous research illustrated that goats can be conditioned to consume more juniper while in individual pens when foraging choices are limited. To test whether this creates a longer-lasting increase in juniper preference, we determined if goats would continue to consume juniper on pasture for one year after being fed juniper in individual pens for 14 d. Female Boer-cross goats (n = 40) were randomly divided into two treatments: conditioned and naive to juniper. At approximately 12 mo of age, conditioned goats were placed in individual pens and fed redberry juniper 1 h daily for 14 d, while naive goats received only alfalfa pellets to meet maintenance requirements. After the pen-feeding phase of the study, goats were placed in one of four pastures (10 goats · pasture^?1) for 12 mo. Two pastures housed conditioned goats, and two pastures housed naive goats at a moderate stocking rate (1 animal unit · yr^?1 · 8 ha^?1). Bite count surveys were conducted twice per month, while herbaceous standing crop and monoterpene levels were measured once per month. Juniper preference varied monthly; however, conditioned goats consistently ate more (P < 0.05) juniper than naive goats except for April, when the study began, and March, when the study ended. When selection of herbaceous forages decreased, conditioned goats increased selection of juniper, while naive goats increased selection of other palatable shrubs. Seasonal changes of monoterpene levels in juniper had no apparent effect on juniper preference. We contend that feeding juniper at weaning will increase use of the plant in grazing situations.     

The visual impact of gossip

Gossip is a form of affective information about who is friend and who is foe. We show that gossip does not influence only how a face is evaluated--it affects whether a face is seen in the first place. In two experiments, neutral faces were paired with negative, positive, or neutral gossip and were then presented alone in a binocular rivalry paradigm (faces were presented to one eye, houses to the other). In both studies, faces previously paired with negative (but not positive or neutral) gossip dominated longer in visual consciousness. These findings demonstrate that gossip, as a potent form of social affective learning, can influence vision in a completely top-down manner, independent of the basic structural features of a face.     

Antimicrobial resistance, virulence profiles, and phylogenetic groups of fecal Escherichia coli isolates: a comparative analysis between dogs and their owners in Japan

In this study, fecal Escherichia coli isolates (n=188) from 34 dog-owner pairs and 26 healthy control humans (2 isolates per individual) were tested for susceptibility to 6 antimicrobials and screened for virulence genes. Genetic diversity between canine and owner isolates was evaluated by pulsed-field gel electrophoresis (PFGE). Canine isolates exhibited significantly different rates of resistance to four and two antimicrobials, compared to control and owner isolates, respectively. Of the genes examined, the prevalence of sfa, hly, and cnf genes in canine isolates were higher than in control isolates, but not than in owner isolates. These results suggest that characteristics of owner isolates are somewhat similar to canine isolates, compared to isolates from non-dog owners. In addition, PFGE analysis revealed that transfer of E. coli between owners and their dogs had occurred within 3/34 (8.8%) households. Considering the effects of dog ownership on the population of E. coli isolates from owners, further epidemiological studies are required.     

Divergent cytokine responses of macrophages to Mycobacterium avium subsp. paratuberculosis strains of Types II and III in a standardized in vitro model

Based on epidemiological and clinical observations, different strains of Mycobacterium avium subsp. paratuberculosis (MAP) are suspected to significantly differ in their virulence for ruminants. In the pathogenesis of paratuberculosis, macrophages represent the principal target cell for MAP. In order to judge the ability of different MAP-genotypes to modulate macrophage responses, the cytokine responses of the monocyte cell line THP-1 were studied after challenge with three different MAP strains under standardized conditions. The bovine field isolate J1961 (major Type II) and the ovine field isolate JIII-86 (Type III) were compared with the laboratory adapted reference strain ATCC 19698 (Type II). Strains were shown by three different typing methods (IS900-RFLP-, MIRU-VNTR-, and SSR-analysis) to substantially differ in several genotypic features. Macrophage function was assessed by quantifying mRNA of the cytokines TNF-α, IL-1?, and IL-10 by quantitative RT-PCR. Secreted TNF-α protein was measured by a cytotoxicity test, IL-1? and IL-10 using ELISA tests. The three MAP strains of various genotypes differ in their effect on human macrophages depending on challenge dose and infection time. These differences concerned both the mRNA level and secreted protein amounts of proinflammatory cytokines TNF-α, IL-1β and anti-inflammatory cytokine IL-10. Type III strain produced less IL-10 and IL-1β mRNA and protein but more TNF-α protein at 2h than the Type II strains. In summary, our results support the hypothesis that strain characteristics might have relevance for the host response towards MAP and, consequently, for the pathogenesis of paratuberculosis.     

Seroepidemiology of respiratory (group 2) canine coronavirus, canine parainfluenza virus, and Bordetella bronchiseptica infections in urban dogs in a humane shelter and in rural dogs in small communities

This prospective study evaluated seroepidemiologic features of canine respiratory coronavirus (CRCoV), canine parainfluenza virus (CPIV), and Bordetella bronchiseptica infections in dogs in an urban humane shelter and in rural/small community dog populations in western Canada. Seroprevalence of CRCoV and CPIV was low compared with other countries; seroprevalence of B. bronchiseptica was moderate to high in most populations examined. Rural dogs were 0.421 times (P ≤ 0.0001) less likely to be positive for CRCoV than dogs admitted to the shelter. There were no statistical differences in prevalence of antibodies to B. bronchiseptica and CPIV between urban and rural populations. Dogs from Fort Resolution, NWT were significantly (P < 0.05) less likely to have moderate or high antibody titers to the 3 agents than dogs in the shelter. Seroconversion to CRCoV was common in dogs in the shelter, but was not associated (P = 0.18) with respiratory disease. Antibodies to CRCoV, CPIV, or B. bronchiseptica on arrival were not significantly (P > 0.05) associated with disease-sparing after entry into the shelter.