Effect of Tillage and Plant Residue Management Practices on Shrinkage of a Vertisol

A study was carried out to determine the effects of different tillage and plant residue management practices on different soil moisture and shrink-swell properties of a Vertisol (very fine, semctitic, thermic, chromic Haploxerert (with less than 1 % slope). The core samples were collected in July (after harvesting of lentil) and in November (after planting of wheat) 1993. The two tillage methods were moldboard (T1) and chisel (T2). The two plant residue incorporation dates were in August (R1) and in October (R2). Results indicated that T2 tends to give higher water holding capacity and available moisture than T1. Immediate incorporation of plant residue (R1) tends to increase the bulk density and to decrease soil specific volume and void ratio when compared to late incorporation treatment (R2). In general, the two tillage treatments tend to decrease moisture availability, shrinkage characteristics, soil specific volume, and void ratio. They also tend to increase the soil shrinkage (subsidence and cracks volume) and bulk density. Chisel plough is recommended in Vertisols, if it is to be used before the rainfall.     

Effect of Tillage System on Soil Strength and Bulk Density of Vertisols

Improving the soil environment for seedling emergence and plant growth is possible using a good tillage system. From March 1993 until June 1994, the effect of tillage system and plant residue incorporation practices on the bulk density and the soil strength of the surface layer of a vertisol (very fine, smectitic, thermic, chromic Haploxerert), on a less than 1 % slope, was investigated. The two tillage methods were moldboard (T1) and chisel (T2). The two plant residue incorporation dates were in August (R1) and in October (R2). Results indicated that R1 gave significantly higher bulk density than R2, while the different tillage treatments had no significant effect. Soil strength was significantly lower under T1 and R2 than under the other treatments.     

Modeling daily soil water dynamics during vertical drainage using the incoming flow concept

Crop management models require simulation of daily soil water dynamics. The objective of this study was to develop a model to simulate the daily soil water dynamics during vertical drainage with reasonable accuracy using the incoming flow concept. The execution of this model, which has been developed based on the conservation of mass law, consists of two steps. First, calculating the potential daily change of soil water content (Δθp) for each soil layer in the profile assuming each one receives no water from the above layer. Then, calculating the actual daily change of soil water (Δθa) for each soil layer in the profile by adjusting Δθp using the incoming water flow, which can be defined as the amount of drainage water that reaches a layer in a soil profile from the above layer. The model was compared with the Suleiman and Ritchie [Suleiman, A.A., Ritchie, J.T., 2004. Modifications to the DSSAT vertical drainage model for more accurate soil water dynamics estimation. Soil Sci. 169 (11), 745–757] vertical drainage model (SRVDM) and HYDRUS-1D for diverse soils and was tested using drainage experimental data of a Eutric Regosol in Bekkevoort, Belgium and a sandy soil in Georgia, U.S. The difference in Δθp between the new model and HYDRUS-1D for diverse soils ranged from − 0.01 to 0.016 m³ m^− 3 for the first day and from − 0.005 to − 0.025 m³ m^− 3 for the second day while the difference in Δθp between the SRVDM and HYDRUS-1D for these soils ranged from 0.014 to 0.062 m³ m^− 3 for the first day and from − 0.01 to 0.026 m³ m^− 3 for the second day. The relative maximum absolute errors in Δθa between the new model and HYDRUS-1D was 10% while the relative maximum absolute errors in Δθa between the SRVDM and HYDRUS-1D was 112%. In the experiments, the root mean square difference of the soil water content for the new model was lower than that for the SRVDM at the different soil depths. These results indicated that the new model outperformed the SRVDM in simulating Δθp and Δθa for diverse soil. It can be concluded that the new model was robust and reasonably accurate for diverse soils at different soil depths. The implementation of such model will improve the accuracy and applicability of regional soil water dynamics simulation and will reduce considerably the computational time and the required inputs.     

Quercetin attenuates H2O2‐induced toxicity of rooster semen during liquid storage at 4°C

Current study was carried out to examine the protective effects of quercetin against toxicity induced by hydrogen peroxide in rooster semen in vitro. Semen samples were collected from ten roosters (Ross 308 broiler breeder males, 32 weeks old) twice a week by abdominal massage method. Samples with ≥70% progressive motility were selected, pooled, diluted and used for the study. Experimental groups consisted of negative control, control that received solvent of quercetin, H₂O₂ (40 μM) and combination groups which incubated with constant dose of H₂O₂ (40 μM) plus various levels of quercetin (20, 40 and 80 μM). Measurement of total hydroperoxide (HPO), malondialdehyde (MDA), nitric oxide (NO), total antioxidant capacity (TAC) and superoxide dismutase activity as well as routine sperm tests were done at 0, 24 and 48 hr of storage at 4°C. Results revealed that exposure to hydrogen peroxide significantly increased HPO (138.43 ± 7.32 vs. 66.08 ± 3.97 μmol/g protein), MDA (7.21 ± 0.08 vs. 5.71 ± 2.16 μmol/g protein) and NO (0.367 ± 0.013 vs. 0.215 ± 0.011 μmol/g protein) levels and decreased sperm progressive motility (27.28 ± 1.21 vs. 47.49 ± 1.29%), and amounts of TAC (11.49 ± 0.39 vs. 15.70 ± 0.79 mmol/g protein) compared to control at 24 hr (p < 0.05). Changes at mentioned variables were repeated at 48 hr of storage. Also, co‐administration of quercetin (especially at 40 and 80 μM) with hydrogen peroxide restored the toxic effects of hydrogen peroxide on rooster semen parameters such as primary and secondary lipid peroxidative indicators and other evaluated variables. The study concluded that rooster semen enrichment with quercetin would protect lipid peroxidative and nitrosative hydrogen peroxide‐mediated damage during cold liquid storage of rooster semen.     

Thermal conductivity and diffusivity of wood

Summary Transient simultaneous measurements of thermal conductivity and diffusivity of Swedish wood have been performed with the plane source technique on oven-dry hardwood (birch) samples at room temperature and at 100 °C. The influences of temperature, density, porosity and anisotropy on thermal conduction were investigated. The measurements were done in longitudinal (parallel to the grain) and transverse (intermediate between radial and tangential) directions. As the temperature increased from 20 to 100 °C, the thermal conductivity of each sample increased slightly for both longitudinal and transverse directions. The effect of density and porosity on the thermal conductivity may be related to the presence of other scattering mechanisms such as voids and cell boundaries. It seems that the dominant mechanism of heat transfer across the cell lumina in these types of wood is the heat conduction through the voids. An attempt was made to explain the behaviour of the effective thermal conductivity by adopting a model based on the ratio between heat conduction in parallel and serial layers of gas, liquid, and solid phases. Received 7 May 1997     

Assessment of nitrogen mineralization potential and availability from neem seed residue in a savanna soil

 Nitrogen (N) mineralization and availability from neem seed residue after oil extraction was studied in a laboratory incubation and greenhouse cropping. Several decomposition models were tested for estimating potentially mineralizable N and mineralization rates from the residue. Net N mineralization was best described by a Gompertz function and a mixed-order rate model with R ²=0.996 for each and residual mean square error (RMSE)=8.3 for the Gompertz function and 8.8 for the mixed-order rate model. A consecutive reaction model also fitted the data closely (R ²=0.983; RMSE=16.6) and is preferable to a Gompertz function or a mixed-order rate model because of its mechanistic basis. Potentially mineralizable N estimated by the decomposition models ranged from 335 to 489 mg N kg^–1 representing between 32% and 43% of total N applied. Actual cumulative N mineralized in a 98-day incubation period was 339 mg N kg^–1 soil. Bio-available N from neem residue and inorganic N (urea) with maize as a test crop in a greenhouse cropping gave similar biomass yield and N uptake, suggesting rapid N mineralization from neem residue to meet plant nutrition. Received: 15 July 1998     

Sperm motility and seminal plasma characteristics in Barbus sharpeyi (Günther, 1874)

Spermatozoa concentration, ionic composition, osmolality, glucose and total protein contents of seminal plasma and sperm motility were determined in Barbus sharpeyi (Cyprinidae, Teleosotei). Spermatozoa concentration ranged from 9.77 to 20.20 × 10⁹ spermatozoa mL^?1. Osmolality (mOsmol kg^?1) and ionic contents (mM L^?1) of the seminal plasma were 274.5±9.0, 70.0±3.4 Na⁺, 28.8±0.9 K⁺, 101.7±3.1 Cl^?, 0.9±0.1 Mg²⁺ and 2.1±0.1 Ca²⁺ respectively. Total protein and glucose were 5.3±0.2 g L^?1 and 76.7±4.3 mM L^?1 respectively. Sperm motility was initiated in a hypo‐osmotic condition, composed of either an ionic (KCl or NaCl) or a non‐ionic (sucrose) activation medium. Duration of sperm motility was very short: <2 min after activation in distilled water. Percentage of motile spermatozoa was significantly higher in an activation medium containing NaCl compared with that of distilled water. An activating medium containing NaCl or KCl higher than 150 mM or sucrose higher than 275 mM totally inhibited the activation of sperm motility. Immediately after sperm activation, wave(s) propagated along the flagellum, but waves were restricted to the proximal part of the flagellum (close to the head) at 1 min post activation. Studied characteristics in the present study were compared with those of other cyprinids for understanding inter‐species differences.     

Production of an Antibody Fragment (scFv) Targeting PcrV Protein of Pseudomonas aeruginosa in Fed-Batch Cultivation Mode

Background: Pseudomonas aeruginosa is one of the opportunistic pathogens causing frequent hospital-acquired life-threatening infections in mechanically ventilated patients. The most significant virulence factor of P. aeruginosa is T3SS. PcrV is an important structural protein of the T3SS. Methods:In the current investigation, a recombinant scFv mAb against the PcrV protein was expressed in EnBase® (fed-batch) cultivation mode. The pETiteTM N-His SUMO Kan vector, including anti-PcrV scFv gene, was transformed into Escherichia coli (BL21) cells. The expression and solubility of anti-PcrV scFv protein were investigated at two different temperatures (25 °C and 30 °C) and at different induction times (4, 6, 8, 12, and 24 hours). Results:Increased efficiency was achieved by EnBase® compared to LB broth; owing to the slow release of glucose, the maximum level of solubility and total protein expression was observed in EnBase® cultivation system at 30 °C and 24 h post induction. Furthermore, IC₅₀ for anti-PcrV scFv protein was determined to be approximately 7 μg/mL. Conclusion:Anti-PcrV scFv produced in this study showed promising in vitro results, protecting RBC from lysis by P. aeruginosa (exoU+). Key Words: Fed Batch, recombinant protein, Pseudomonas aeruginosa, scFv     

Mucoadhesive Chitosan Electrospun Nanofibers Containing Tetracycline and Triamcinolone as a Drug Delivery System

The mucoadhesive Chitosan (CS) nanofibers as a drug delivery system were developed. Chitosan was modified via the immobilization of thiol groups from L-cysteine as a mucoadhesive reagent. The mucoadhesive properties of the chitosan nanofibers were evaluated by tensiometer set and via tensile studies. Drug and mucoadhesive agent loading lead to decrease diameters and increased porous of nanofibers. The release of Tetracycline (Tet) and Triamcinolone (Tri) were increased with increasing immersion time and it became constant at long immersion times. Mucoadhesion studies were done at pH 2–7 and in pH 6 maximum mucoadhesive properties observed. Release studies demonstrated a sustained release of both drug continued up to 48 hours. Microbial studies were performed on the nanofibers. The drug delivery system represented a novel tool for improve the therapeutic efficacy of various drugs that are poorly absorbed from the gastrointestinal tract. Also it is an efficient system for treatment of oral ulceration.