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51.
AM Batista WA Gomes CCD Carvalho PLJ Monteiro Jr FLM Silva FC Almeida PC Soares GF Carneiro MMP Guerra 《Reproduction in domestic animals》2014,49(3):476-480
The aim of this study was to evaluate the effect of leptin administration during superovulation on in vivo goat embryo production. Ten mature does were superovulated with 133 mg follicle‐stimulating hormone (FSH) i.m. in six descending doses at 12‐h intervals. The goats received 4.8 μg/kg human recombinant leptin s.c. (leptin group, n = 5) or phosphate‐buffered saline (PBS) (control group, n = 5) with the first and second FSH doses. The does were mated and subjected to embryo collection by transcervical technique 6 days later. The total number of cells per embryo and the number of cells with fragmented DNA were assessed in selected blastocysts by combining Hoechst 33342 and terminal dUTP nick‐end labelling (TUNEL) staining. Plasma concentrations of oestradiol (E2) and progesterone (P4) were determined by electrochemiluminescence from the day of FSH treatment, on the day of superovulatory oestrus and on the day before embryo collection. Compared with the control group, the does that received leptin had a higher number of transferable embryos (p < 0.005), fewer embryos classified as degenerated (p < 0.001) and fewer TUNEL‐positive cells/blastocyst (p < 0.001). The number of transferable embryos was positively correlated with E2 concentrations on day of oestrus (r = 0.562; p < 0.01) and P4 concentrations on the day of embryo collection (r = 0.912; p < 0.001). We concluded that in vivo leptin administration during FSH treatment improved embryo quality and affected ovarian steroidogenesis in superovulated goats. 相似文献
52.
Steve Edwards 《Aquaculture Research》2003,34(14):1361-1365
The early physiological response (3 days) of greenlip (Haliotis laevigata Leach) and blacklip (H. rubra Leach) abalone to a single abrupt change in salinity was monitored using a multi‐channel open‐circuit respirometer. The range of salinity tolerance for both species in this trial was 25–40 ppt. Indications from other trials were that a margin of 2 ppt outside this range will cause mortality. Serum volume was increased by reduced salinity as much as 25% in the short term, with equilibration of the concomitant whole body weight increase (9.2±0.5%) occurring within 1 h. The serum volume appeared to be decreased at high salinity. There was little underlying change in basal oxygen usage levels, but significant behavioural changes that affected overall oxygen consumption. Both high and low salinity appeared to reduce activity. Animals in low salinity exhibit partial recovery of activity levels after 1 day in a manner similar to other stress responses. Animals at high salinity (40 ppt) did not show recovery of activity levels over 3 days. Overall, these results suggest that greenlip and blacklip abalone will have little trouble tolerating moderately low‐salinity environments. 相似文献
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Traditional artisanal aquaculture systems are commonly assumed to be mainly for subsistence, to use predominantly on-farm inputs, and to have been developed by farmers themselves. Such systems with a long history in South East Asia exist mainly in northern Lao PDR, northern Vietnam and in West Java. In most other areas the traditional fish supply, wild fish, has declined only relatively recently, providing a stimulus for growth of aquaculture over the past few decades. An overview of artisanal aquaculture so defined in the South East Asian region is presented from a systems context considering social and economic aspects (micro- and macro-level perspectives), production technology (rice fields, ponds, cages), and environmental aspects (fitting into the local resource base without adverse environmental impact). Most artisanal aquaculture systems are integrated with crops and livestock but generally resource-poor farms constrain production. Rising expectations mean that productivity must be enhanced by off-farm inputs for aquaculture to contribute significantly to the farm household livelihood system. It is proposed that the term ‘small-scale’ be used rather than ‘artisanal’ because of increasing farmer interest in income rather than subsistence, because of increasing use of off-farm inputs, and because of the increasingly important role of science in the promotion of such systems. 相似文献
55.
James O Harris Christopher M Burke Stephen J Edwards & Deon R Johns 《Aquaculture Research》2005,36(14):1400-1407
Growth and survival of juvenile greenlip (39.03 (SD 3.80) mm (n=524)) and blacklip (31.92 (SD 4.19) mm (n=531) abalone were investigated at high dissolved oxygen levels (95–120% saturation) between 17 and 19°C. Abalone were fed the same artificial diet and each species was contained in groups of approximately 30 individuals within triplicate tanks for each of six treatments and were exposed to flow through water adjusted to give experimental conditions for up to 75 days. Blacklip abalone held at 16.9°C and 97% oxygen saturation grew in shell length significantly faster than all other treatments of blacklip abalone held at 19°C, and significantly faster than blacklip abalone maintained at 111% oxygen saturation and 17.5°C. Both temperature and oxygen saturation significantly affected the survival of this species. Blacklip abalone held at 19°C had significantly lower survival for both 96% oxygen saturation and 120% oxygen saturation, compared with blacklip abalone maintained at either 110% oxygen saturation and 19°C, or for any 17°C treatment. No significant differences were noted for greenlip abalone within the range tested in terms of growth rate, food consumption rate or survival, indicating that greenlip abalone tolerated these conditions better than did blacklip abalone. 相似文献
56.
A study of the environmental effects associated with the trestle cultivation of Pacific oysters, Crassostrea gigas Thunberg, was conducted at a commercial cultivation site in the River Exe estuary, Devon, England. Small, but significant, changes were detected in the macrofaunal community sampled beneath oyster trestles compared with that found in adjacent uncultivated areas. These changes were associated with an increase in organic and silt composition and a reduction in the depth of the oxygenated layer of the sediment beneath the trestles. Water velocity was decreased by the presence of the trestles which probably led to the increase in sedimentation rate observed beneath them. Although biological and physical changes were observed, they were relatively minor compared with the extreme environmental changes associated with the suspended culture techniques used for other bivalve species and fishes. However, other studies suggest that the environmental effects associated with oyster cultivation become more severe in areas of large-scale (hectares) cultivation. 相似文献
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During winter when the active layer of Arctic and alpine soils is below 0 °C, soil microbes are alive but metabolizing slowly, presumably in contact with unfrozen water. This unfrozen water is at the same negative chemical potential as the ice. While both the hydrostatic and the osmotic components of the chemical potential will contribute to this negative value, we argue that the osmotic component (osmotic potential) is the significant contributor. Hence, the soil microorganisms need to be at least halotolerant and psychrotolerant to survive in seasonally frozen soils. The low osmotic potential of unfrozen soil water will lead to the withdrawal of cell water, unless balanced by accumulation of compatible solutes. Many microbes appear to survive this dehydration, since microbial biomass in some situations is high, and rising, in winter. In late winter however, before the soil temperature rises above zero, there can be a considerable decline in soil microbial biomass due to the loss of compatible solutes from viable cells or to cell rupture. This decline may be caused by changes in the physical state of the system, specifically by sudden fluxes of melt water down channels in frozen soil, rapidly raising the chemical potential. The dehydrated cells may be unable to accommodate a rapid rise in osmotic potential so that cell membranes rupture and cells lyse. The exhaustion of soluble substrates released from senescing plant and microbial tissues in autumn and winter may also limit microbial growth, while in addition the rising temperatures may terminate a winter bloom of psychrophiles.Climate change is predicted to cause a decline in plant production in these northern soils, due to summer drought and to an increase in freeze-thaw cycles. Both of these may be expected to reduce soil microbial biomass in late winter. After lysis of microbial cells this biomass provides nutrients for plant growth in early spring. These feedbacks, in turn, could affect herbivory and production at higher trophic levels. 相似文献
59.
K. Zemanová T. Picek J. Dušek K. Edwards H. Šantrůčková 《Water, air, and soil pollution》2010,207(1-4):39-48
Two horizontal constructed wetlands with subsurface flow (CWs) of different age were monitored in a 2-year study. One of the CWs was new, while the second one had been in operation for 5 years in the first year of study. Transformations of C, P, and N were measured in each CW in the substrate of the vegetated bed under both aerobic and anaerobic conditions, and their rates were compared. C was mineralized in both CWs under both aerobic and anaerobic conditions, but mineralization rates differed between the CWs; they were cca ten times higher in the established CW compared to the new CW. Dissolved reactive phosphorus (DRP) was immobilized under aerobic conditions but was mobilized under anaerobic conditions. DRP transformation was cca five times faster in the established CW. Nitrification occurred under aerobic conditions at similar rates in both CWs. NH4 + concentration decreased under both aerobic and anaerobic conditions, but there was large variability. The age of the CW affected C mineralization rates and DRP immobilization rates under aerobic conditions and mobilization rates under anaerobic conditions; they increased as the CWs maturated, while no effect of CW age was observed on nitrogen removal rates. 相似文献
60.
The effects of five years of application of N, P and K fertilizers at three levels, and of agricultural lime at two levels, on the herbage yield of Eragrostis curvula are discussed. The experiment was carried out on leached soils under an average annual rainfall of 900mm. Significant responses in yield were obtained to N, P and K fertilizers but there was no response to lime. 相似文献