Feeding and vigilance behaviour of breeding ostriches (Struthio camelus) in a farming environment in Britain

1. Vigilance and feeding behaviour of male and female adult breeding ostriches were recorded to determine feeding and scanning bout lengths, a time budget and the pattern of vigilance immediately after food was provided. 2. Males were more vigilant and fed for shorter periods than females immediately after concentrate food was delivered but not throughout the whole day. 3. Most interscan periods of males were below 40 s with a maximum of 90 s compared with most interscan periods of females lasting less than 70 s with a maximum of 160 s. 4. Gender differences in behaviour are attributed to increased male vigilance for predators and/or conspecifics, and increased female feeding required for egg production and greater opportunity to feed because of male vigilance.     

Isolation and structure of higher diamondoids,nanometer-sized diamond molecules

We exploited the exceptional thermal stability and diverse molecular shapes of higher diamondoids (C22 and higher polymantanes) to isolate them from petroleum. Molecules containing 4 to 11 diamond-crystal cages were isolated and crystallized, and we obtained single-crystal x-ray structures for representatives from three families. Rigidity, strength, remarkable assortments of three- dimensional shapes, including resolvable chiral forms, and multiple, readily derivatizable attachment sites make them valuable nanometer-size molecular building blocks.     

Rapid method for extracting aldrin, dieldrin, and endrin from visceral material.

A simple method is described for purifying aldrin, dieldrin, and endrin from visceral material by using a Celite column. Celite retains all interfering materials including Lipids whereas the chlorinated Insecticides are eluted from the column with n-hexane. This extract can be used directly for gas chromatographic analysis.     

Chitinase production by an Arthrobacter sp. lysing cells of Fusarium roseum

An Arthrobacter sp. which actively lysed Fusarium roseum was found to liberate chitinase (E.C. 3.2-1.14, chitin glycanohydrolase), an enzyme essential for the hydrolysis of chitin, a major component of fusarial hyphal walls. Factors involved in the production of chitinase were investigated by modifying culture conditions and assaying for enzyme activity. Production occurred on colloidal chitin as well as on native chitin supplemented with yeast extract or peptone. Enzyme production paralleled growth; liberation of enzyme took place during the log phase with the maximum yield being obtained at the stationary phase. Addition of the non-ionic surfactant, polyoxyethylene sorbitan monooleate (Tween 80) increased enzyme yield. An inverse relationship was found between the amount of enzyme produced and the quantity of n-acetyl-glucosamine liberated. The enzyme was generally not produced when grown on various other carbohydrates. These findings suggest that chitinase is inducible and that chitin breakdown is regulated by a repressor-inducer mechanism.Initial hydrolysis rates of colloidal chitin were proportional to the concentration of chitinase used. Optimal pH and temperature for enzyme activity were 4.9 and 50°C, respectively. Purification of the chitinase was obtained by (NH₄)₂SO₄ precipitation followed by DEAE-cellulose and Sephadex chromatography, achieving a 12-fold increase in specific activity.     

Spatial scale effects on conservation network design: trade-offs and omissions in regional versus local scale planning

Ecological patterns and processes operate at a variety of spatial scales. Those which are regional in nature may not be effectively captured through the combination of conservation plans derived at the local level, where land use planning frequently takes place. Conversely, regional conservation plans may not identify resources important for conservation of intraregional ecological variation. We compare modeled conservation networks derived at regional and local scales from the same area in order to analyze the impact of scale effects on conservation planning. Using the MARXAN reserve selection algorithm and least cost corridor analysis we identified a potential regional conservation network for the Central Valley ecoregion of California, USA, from which we extracted those portions found within five individual counties. We then conducted the same analysis for each of the five counties. An overlay of the results from the two scales shows a general pattern of large differences in the identified networks. Especially noteworthy are the trade-offs and omissions evident at both scales of analysis and the disparateness of the identified corridors that connect core reserves. The results suggest that planning efforts limited to one scale will neglect biodiversity patterns and ecological processes that are important at other scales. An intersection of results from the two scales can potentially be used to prioritize areas for conservation found to be important at several spatial scales.