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Clear, stable gels have been prepared from purified tropocollagen from calf skin; the collagen was solubilized with a proteolytic enzyme (Proctase) and stabilized by ultraviolet irradiation under nitrogen. These gels are clear, possess altered immunologic reactivity, and have properties of an ideal vitreous replacement. Implantations in rabbit and monkey eyes appear to be well tolerated, remain clear, and gradually disappear in about 2 months. 相似文献
104.
Bioluminescence (light production) and fluorescence (re-emission of absorbed radiation as light) are found in an unaccountably diverse array of marine organisms, where their functions are largely unknown. Here we report a deep-sea siphonophore that twitches glowing lures to attract fish. This is rare evidence of bioluminescence used for prey attraction among nonvisual marine organisms. The lures also contain red fluorescent material that shifts the wavelength of emitted light. The existence of a red-luminescent invertebrate suggests that long-wavelength light plays a greater role in marine interactions than previously suspected. 相似文献
105.
Gene encoding the beta subunit of S100 protein is on chromosome 21: implications for Down syndrome 总被引:17,自引:0,他引:17
R Allore D O'Hanlon R Price K Neilson H F Willard D R Cox A Marks R J Dunn 《Science (New York, N.Y.)》1988,239(4845):1311-1313
S100 protein is a calcium-binding protein found predominantly in the vertebrate nervous system. Genomic and complementary DNA probes were used in conjunction with a panel of rodent-human somatic cell hybrids to assign the gene for the beta subunit of S100 protein to the distal half of the long arm of human chromosome 21. This gene was identified as a candidate sequence which, when expressed in the trisomic state, may underlie the neurologic disturbances in Down syndrome. 相似文献
106.
A whole-genome assembly of Drosophila 总被引:1,自引:0,他引:1
Myers EW Sutton GG Delcher AL Dew IM Fasulo DP Flanigan MJ Kravitz SA Mobarry CM Reinert KH Remington KA Anson EL Bolanos RA Chou HH Jordan CM Halpern AL Lonardi S Beasley EM Brandon RC Chen L Dunn PJ Lai Z Liang Y Nusskern DR Zhan M Zhang Q Zheng X Rubin GM Adams MD Venter JC 《Science (New York, N.Y.)》2000,287(5461):2196-2204
We report on the quality of a whole-genome assembly of Drosophila melanogaster and the nature of the computer algorithms that accomplished it. Three independent external data sources essentially agree with and support the assembly's sequence and ordering of contigs across the euchromatic portion of the genome. In addition, there are isolated contigs that we believe represent nonrepetitive pockets within the heterochromatin of the centromeres. Comparison with a previously sequenced 2.9- megabase region indicates that sequencing accuracy within nonrepetitive segments is greater than 99. 99% without manual curation. As such, this initial reconstruction of the Drosophila sequence should be of substantial value to the scientific community. 相似文献
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108.
Dunn G 《Science (New York, N.Y.)》1930,71(1837):276-277
109.
Long-term neuropathological and neurochemical effects of nucleus basalis lesions in the rat 总被引:1,自引:0,他引:1
The long-term effects of excitotoxic lesions in the nucleus basalis magnocellularis of the rat were found to mimic several neuropathological and chemical changes associated with Alzheimer's disease. Neuritic plaque-like structures, neurofibrillary changes, and neuronal atrophy or loss were observed in the frontoparietal cortex, hippocampus, amygdala, and entorhinal cortex 14 months after the lesions were made. Cholinergic markers in neocortex were reduced, while catecholamine and indoleamine metabolism was largely unaffected at this time. Bilateral lesions of the nucleus basalis magnocellularis increased somatostatin and neuropeptide Y in the cortex of the rat by at least 138 and 284 percent, respectively, suggesting a functional interaction between cholinergic and peptidergic neurons that may differ from that in Alzheimer's disease. 相似文献
110.
High-frequency C-type virus induction by inhibitors of protein synthesis 总被引:16,自引:0,他引:16
When inhibitors of protein synthesis are added to BALB/c mouse cells in culture, induction of naturally integrated C-type RNA virus occurs in a high percentage of cells. The action of protein synthesis inhibitors differs from that of halogenated pyrimidines, another class of virus inducers, in their effects on biologically distinguishable viruses. The use of such inhibitors to study integrated virus expression provides a means for studying gene regulation in mammalian cells. 相似文献