5株水貂阿留申病毒的分离与鉴定

为给水貂阿留申病疫苗的研制奠定基础,对疑似感染阿留申病死亡的水貂内脏处理后,接种猫肾传代细胞（CRFK）分离病毒,并对分离的毒株进行PCR鉴定及动物回归试验。与此同时,首次应用免疫过氧化物酶单层细胞实验（IPMA）对所分离的水貂阿留申病毒进行TCID50的测定。结果成功分离并鉴定出5株水貂阿留申病毒株,分别命名为ADV-DL124、ADV-DL125、ADV-ZJ3、ADV-QD2、ADV-QD3,各分离株的TCID50分别为105.7TCID50/ml、105.0TCID50/ml、104.6 TCID50/mL、105.2 TCID50/mL、104.1 TCID50/mL。动物回归实验显示,所分离的病毒对水貂具有致病性,为水貂阿留申病毒强毒株。     

Promoter identification and effect on activation of NF-κB of porcine ISG58

Interferon (IFN)-stimulated gene (ISG) 56 family (composed of ISG54, ISG56, ISG58, and ISG60) plays important roles in defense against viral infection in mammalian cells. Numerous studies have been conducted on ISG54, ISG56, and ISG60; however, little is known on ISG58. In the present study, the upstream sequence of porcine ISG58 gene was first characterized as functional promoter by luciferase reporter assay, and then two directly adjacent IFN-stimulated response elements (ISREs), one at ?206 to ?194 (ISRE-I) and a second one, directly upstream of this element at ?219 to ?207?bp (ISRE-II), were identified using the bioinformatics method. The subsequent site-directed deletion and transient transfection experiments showed the candidate ISREs are functional. ISRE-I works better than ISRE-II and synergistic cooperation exists between two ISREs. Additionally, the effect of porcine ISG58 on activation of NF-κB was analyzed using the dual-luciferase reporter assay. The results will contribute to revealing the role of ISG58 in immune response.     

Immunity of tick-exposed seronegative and seropositive small stock challenged with two stocks of Cowdria ruminantium

Nineteen per cent and 32% of serologically positive sheep and goats in heartwater endemic areas of the Republic of South Africa were susceptible to challenge with, respectively, the Ball 3 and Welgevonden stocks of Cowdria ruminantium. There was good correlation between the results of the indirect fluorescent antibody test and immunity, since without exception all the seronegative animals reacted when they were challenged. The fact that only 74% of the seropositive sheep and goats were immune to challenge can probably be ascribed to the poor cross-protection between stocks of C. ruminantium and not to false positive serological reactions.     

百脉根大田种子生产的技术研究

在准噶尔盆地北缘海拔640m台地灌溉区新垦荒地上,通过对加拿大里奥百脉根6年的种子生产并结合小区试验,总结了整地、播种期、播种密度、灌溉、调节灌溉、施肥、收获种子、切割松耙等行之有效的方法,保证了大田种子生产丰产丰收,具有可靠、实用的推广价值.     

新疆西昆仑山禾本科植物物种多样性特点

通过实地考察和植物标本的采集与鉴定,并结合相关文献资料的查阅,对新疆西昆仑山禾本科植物物种多样性特点进行了研究。结果表明,新疆西昆仑山的禾本科植物共计37属137种,分别占新疆禾本科植物属、种总数的52.11%和42.55%,属种丰富度比较高;其中发现该区新记录植物6种。本区禾本科植物特有现象不明显,属的组成以仅含1种的属(17属17种)和少种属(13属35种)为主,共计30属52种,分别占属、种总数的81.08%和37.96%;中型属(4属32种)和较大属(3属53种)共计7属85种,分别占属、种总数的18.92%和62.04%,在种级水平占据明显优势,是该区系的主体。区系地理成分有6个分布类型和4个变型,以温带分布及其变型为主。植物生活型以多年生草本(116种,占84.67%)为主,其次为一年生草本(21种,占15.33%)。生态型以中生植物(63种,占45.99%)和旱生植物(61种,占44.53%)为主,显示其与草原、草甸的密切关系,另有少量湿生植物(13种,占9.49%)。说明该地区比较适合禾本科多年生的旱生和中生植物生长,且该区系的禾本科植物地理成分类型与该区所处地理位置为温带较吻合。     

Aberrant extradural spinal migration of Spirocerca lupi: four dogs

Spirocerca lupi is a nematode mainly affecting dogs but has been found in other animals, particularly carnivores. Anatomical areas of typical and aberrant migration vary. This report describes four cases of Spirocerca lupi causing neurological symptoms, similar to thoracolumbar disc syndrome, as a result of aberrant migration of the nematode into the spinal canal. In two of the cases, the nematode could be demonstrated intraoperatively. The third was discovered on post-mortem examination, while the fourth case displayed compelling evidence of Spirocerca lupi involvement. Surgical removal of the Spirocerca lupi nematode would be the treatment of choice in cases of spinal migration, with therapeutic and preventive treatment with doramectin instituted to treat remote sites and prevent re-infection. In areas endemic for spirocercosis, Spirocerca lupi should be considered as an important differential diagnosis in cases that are presented with clinical signs suggestive of a spinal cord lesion.     

正确认识现代养猪生产的市场规律

中国现代养猪业在经历了政府宏观调控的计划经济之后,已逐步走上了以市场规律调节为主的市场经济。尤其在20世纪90年代以后,国内养猪业经过几次周期性波动发展,更明显地表现出市场调节机制在养猪生产中所发挥的作用。2003年6月至2005年7月,养猪生产经历了新一轮的大好形势。200     

结缕草种子打破休眠过程呼吸途径的研究

采用呼吸抑制剂测定了结缕草休眠与打破休眠种子在发芽过程中的各个呼吸代谢途径－糖酵解呼吸途径（ＥＭＰ）、三羧酸循环呼吸途径（ＴＣＡ）、磷酸戊糖呼吸途径（ＰＰＰ）的呼吸速率及其占总呼吸速率的比例，结果表明，休眠种子的总呼吸速率显著低于打破休眠种子的总呼吸速率，造成这种差异的原因是磷酸戊糖途径及三羧酸循环途径的呼吸速率低，这是休眠结缕草种子不能发芽的原因之一。     

2月龄仔猪发生梭菌性肠毒血症的诊治报告

仔猪肠毒血症又名仔猪传染性坏死性肠炎、猪梭菌性肠炎，俗称仔猪红痢。主要发生于1周龄内的新生仔猪，以泻出红色带血稀粪为特征。淮安市楚州区某乡村连续发生多起60日龄左右仔猪以衰竭、瘫痪、昏迷、急性死亡为特征的病例。经流行病学调查、临床症状、尸体剖检、实验室检验，确诊为产气荚膜芽孢梭菌引起的急性肠毒血症。     

H5N1亚型禽流感病毒NS1基因的原核表达及其ELISA检测方法的建立

采用RT-PCR技术扩增了禽流感病毒（AIV）A／Goose／HLJ／p46／2003（H5N1）的NSl基因，将其克隆于融合蛋白表达载体pMAL-c2X上，转化DH5α大肠埃希氏菌感受态细胞，经BamHⅠ和HindⅢ双酶切鉴定及序列分析，表明筛选到了重组质粒pc2X-NS1。SDS-PAGE电泳结果显示，重组质粒转化TB1大肠埃希氏菌后，经0．3mmol／L的IPTG诱导，融合蛋白MBP-NS1得到大量表达，融合蛋白以可溶形式存在，分子质量约为67ku。Western-blotting检测结果表明，融合蛋白MBP-NS1能够与H5N1亚型AIV活病毒感染康复鸭血清发生特异性反应，而不能够与H5N1亚型AIV灭活疫苗免疫鸭血清发生反应。试验初步建立了以纯化的融合蛋白MBP-NS1为包被抗原的间接ELISA检测方法，为AIV灭活疫苗免疫家禽与AIV感染家禽的鉴别诊断奠定了基础。