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101.
Rell L. Parker John Du Richard L. Shinn Adam G. Drury FangChi Hsu John L. Roberston Thomas E. Cecere Avril U. Arendse John H. Rossmeisl 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2022,36(2):694
BackgroundSeizures in the early postoperative period after intracranial surgery may affect outcome in dogs.ObjectivesTo determine the incidence of early postoperative seizures (EPS) in dogs with brain tumors, identify specific risk factors for EPS, and determine if EPS affects outcome.AnimalsEighty‐eight dogs that underwent 125 intracranial surgeries for diagnosis and treatment of rostrotentorial brain tumors.MethodsRetrospective cohort study. All patients with a diagnosis of rostrotentorial brain tumor from 2006 to 2020 were included. Early postoperative seizures were diagnosed by observation of seizure activity within 14 days of neurosurgery. Previously diagnosed structural epilepsy, perioperative anticonvulsant drug (ACD) use, magnetic resonance imaging (MRI), and tumor characteristics were evaluated. Outcome measures included neurologic and nonneurologic complications, duration of hospitalization, and survival to discharge.ResultsDogs with rostrotentorial brain tumors had EPS after 16/125 (12.8%) neurosurgical procedures (95% confidence interval [CI], 7%‐19%). Presence of previous structural epilepsy was not associated with EPS risk (P = 1). Perioperative ACD use also was not associated with EPS (P = .06). Dogs with EPS had longer hospitalization (P < .001), were more likely to have neurologic complications postsurgery (P = .01), and were less likely to survive to discharge (P = .01).Conclusions and Clinical ImportanceIt is difficult to predict which dogs are at risk of EPS because the presence of previous structural epilepsy and the use of perioperative ACDs was not associated with EPS. However, seizures in the early postoperative period are clinically important because affected dogs had prolonged hospitalization, more neurologic complications, and decreased short‐term survival. 相似文献
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草鱼肾组织细胞系CIK的建立及其生物学特性 总被引:20,自引:3,他引:20
作者于1982年1月开始进行草鱼肾组织单层细胞培养,至今已连续培养32个月,传至120多代,建立了细胞系,定名为草鱼肾组织细胞系(CIK)。本文介绍了原代和传代培养、细胞形态、细胞生长速度和分裂指数、细胞的保存和对温度的适应性、细胞染色体分析以及细胞系对病毒敏感性的试验和研究结果。CIK生长迅速、适应性强、以20℃-38℃生长较好,28℃左右生长稳定,pH为6.5时仍能保持致密单层,染色体数为非整倍体,众数为55。对草鱼出血病左右病毒FRV具敏感性。 相似文献
105.
Effects of dietary protein to energy ratios on growth,body composition and digestive enzyme activities in Chinese mitten‐handed crab,Eriocheir sinensis
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Yanyan Cui Qianqian Ma Samwel Mchele Limbu Zhenyu Du Nannan Zhang Erchao Li Liqiao Chen 《Aquaculture Research》2017,48(5):2243-2252
This study aimed to determine the optimal protein to energy ratio (P/E ratio) and evaluate the effect of dietary protein and lipid levels on growth performance, body composition and digestive enzymes activities in Chinese mitten‐handed crab, Eriocheir sinensis. Nine practical diets containing three levels both for protein (DP 30%, 35% and 40%) and lipid (DL 2%, 7% and 12%) with P/E ratios ranging from 13.69 to 19.79 mg KJ?1 were fed to four replicates of crabs (3.39 ± 0.10 g) for 10 weeks. Weight gain increased significantly with the increase in DP level at each DL level. Moreover, weight gain increased in crabs fed with diets containing DL level from 2% to 12% and DP level from 30% to 35%. However, the diet containing 40% DP and 12% DL levels significantly decreased the growth performance and protein efficiency of the crabs. The whole crab and hepatopancreas lipid contents also increased as dietary lipid increased, but not dietary protein. The total protease activity increased significantly with the increase in dietary protein at each lipid level. The lipase activity was statistically comparable among different DL levels at each DP level. Taken together, the crab fed the diet containing 35% protein and 12% lipid levels with P/E 15.77 mg KJ?1 revealed optimal growth, feed utilization efficiency and digestive enzymes activities. Moreover, our study indicated that the higher dietary lipid level at a relatively lower dietary protein level could provide protein sparing effect in Eriocheir sinensis. 相似文献
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Dietary α‐linolenic acid affects lipid metabolism and tissue fatty acid profile and induces apoptosis in intraperitoneal adipose tissue of juvenile grass carp (Ctenopharyngodon idella)
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A 56‐day feeding trial was conducted to elucidate the effects and mechanism action of dietary α‐linolenic acid (ALA, 18:3n‐3) on lipid accumulation and fatty acid profile of muscle, hepatopancreas and intraperitoneal fat (IPF) in juvenile grass carp using three isonitrogenous and isoenergetic semi‐purified diets containing 0.0% (control group), 1.0% and 2.0% ALA, respectively. The lowest intraperitoneal fat (IPF) ratio was found in 2.0% group. In the muscle, hepatopancreas and IPF, docosahexaenoic acid (DHA, 22:6n‐3) and eicosapentaenoic acid (EPA, 20:5n‐3) contents increased with the increase in dietary ALA. In the IPF, caspase 3, caspase 8 and caspase 9 showed the highest activities in 2.0% group, while the value of Bcl‐2/Bax (B‐cell leukaemia 2/Bcl‐2‐associated X protein) reached the lowest. Meanwhile, swelling of the IPF mitochondria was observed in 2.0% group. The gene expressions of fatty acid desaturase (FAD) and fatty acid elongase (ELO) in the hepatopancreas and muscle showed significantly higher levels in the treatment groups, whereas an opposite trend was existed in the IPF. Fatty acid synthase (FAS), sterol regulatory element binding protein‐1c (SREBP‐1c) in the IPF and hepatopancreas reached the lowest in 2.0% group. Overall, dietary ALA could promote n‐3 highly unsaturated fatty acids (HUFAs) synthesis and suppress the accumulation of lipid by decreasing the expression of related genes and promoting the apoptosis in IPF. 相似文献
108.
Relationship between stable carbon isotope discrimination and water use efficiency under regulated deficit irrigation of pear-jujube tree 总被引:2,自引:0,他引:2
Ningbo Cui Taisheng Du Shaozhong Kang Fusheng Li Xiaotao Hu Mixia Wang Zhijun Li 《Agricultural Water Management》2009,96(11):1615-1622
To investigate the relationship between stable carbon isotope discrimination (Δ) of different organs and water use efficiency (WUE) under different water deficit levels, severe, moderate and low water deficit levels were treated at bud burst to leafing, flowering to fruit set, fruit growth and fruit maturation stages of field grown pear-jujube tree, and leaf stable carbon isotope discrimination (ΔL) at different growth stages and fruit stable carbon isotope discrimination (ΔF) at fruit maturation stage were measured. The results indicated that water deficit had significant effect on ΔL at different growth stages and ΔF at fruit maturation stage. As compared with full irrigation, the average ΔL at different growth stages and ΔF at fruit maturation stage were decreased by 1.23% and 2.67% for different water deficit levels, respectively. ΔL and ΔF among different water deficit treatments had significant difference at the same growth stage (P < 0.05). Under different water deficit conditions, significant relationships between the ΔL and WUEi (photosynthesis rate/transpiration rate, Pn/Tr), WUEn (photosynthesis rate/stomatal conductance of CO2, Pn/gs), WUEy (yield/crop water consumption, Y/ETc) and yield, or between the ΔF and WUEy and yield were found, respectively. There were significantly negative correlations of ΔL with WUEi, WUEn, WUEy and yield (P < 0.01) at the fruit maturation stage, or ΔL with WUEi and WUEn (P < 0.01) over whole growth stage, respectively. ΔF was negatively correlated with WUEy, WUEn and yield (P < 0.05), but positively correlated with ETc (P < 0.01) over the whole growth stage. Thus ΔL or ΔF can compare WUEn and WUEy, so the stable carbon isotope discrimination method can be applied to evaluate the water use efficiency of pear-jujube tree under the regulated deficit irrigation. 相似文献
109.
基于图像旋转投影的导航路径检测算 总被引:8,自引:6,他引:2
提出一种农业车辆视觉导航路径识别算法--旋转投影算法.该算法通过角度枚举对图像ROI实施旋转变换,由旋转后图像的列均值与枚举角度构成旋转投影矩阵R,对其行向量实施差分运算得到差分旋转投影矩阵Rd,由Rd的极值可确定图像导航路径,即航向偏差θ与航位偏差d,进而可以求得世界坐标系下的导航路径参数.同理可以对田头线进行检测.为了提高算法的实时性,提出设定合理的ROI、实施线性压缩、旋转角度先粗分再细分的二步法以及充分利用前帧信息4种处理方法,使处理一帧用时6.2ms左右.通过对不同条件下成熟小麦图像测试表明,该算法识别导航路径准确率达到95%. 相似文献
110.