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31.
SUMMARY Thirteen Tammar wallabies (Macropus eugenii) were dosed orally with 500, 1000 or 10 000 oocysts of Toxoplasma gondii, as part of a vaccination trial. Eleven animals died of acute toxoplasmosis 9 to 15 days after challenge. The lesions were similar in all animals, consisting of foci of necrosis and inflammation in the intestines, lymphoid tissue, adrenal cortex, heart, skeletal muscle and brain, and severe generalised pulmonary congestion and oedema. Free and intracellular tachyzoites of Toxoplasma were associated with the lesions. The remaining 2 animals had shown no signs of disease when euthanased four months after challenge. Small, focal, non-suppurative inflammatory lesions were seen in brain, heart and skeletal muscle of these animals and chronic Toxoplasma infection was confirmed by mouse inoculation. 相似文献
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A national serological survey to verify Australia's freedom from porcine reproductive and respiratory syndrome 总被引:4,自引:0,他引:4
MG GARNER LJ GLEESON PK HOLYOAKE RM CANNON WJ DOUGHTY 《Australian veterinary journal》1997,75(8):596-600
Objective To provide serological data to support Australia's claim of freedom from porcine reproductive and respiratory syndrome.
Design A national serological survey was designed to provide 99% confidence of detecting at least one infected pig herd in Australia, assuming that at least 5% of herds would have been exposed to porcine reproductive and respiratory syndrome virus and that at least 25% of the 'finisher' pigs in these herds would have antibodies to the virus.
Procedure A two-stage testing regime was used. All samples were tested with a commercially available enzyme-linked immunosorbent assay. If assay reactions were found, all samples from the herd were to be tested using the indirect immunofluorescence antibody assay.
Results Of the 875 samples from 163 herds from all States in Australia, there was some evidence of reactivity in only four samples from four herds on the enzyme-linked immunosorbent assay. Further testing using the indirect immunofluorescence antibody assay according to the study protocol demonstrated that the reactions were not due to the presence of specific porcine reproductive and respiratory syndrome virus antibodies in the sera.
Conclusion The results of this study support the view that Australian pigs are free of porcine reproductive and respiratory syndrome virus. 相似文献
Design A national serological survey was designed to provide 99% confidence of detecting at least one infected pig herd in Australia, assuming that at least 5% of herds would have been exposed to porcine reproductive and respiratory syndrome virus and that at least 25% of the 'finisher' pigs in these herds would have antibodies to the virus.
Procedure A two-stage testing regime was used. All samples were tested with a commercially available enzyme-linked immunosorbent assay. If assay reactions were found, all samples from the herd were to be tested using the indirect immunofluorescence antibody assay.
Results Of the 875 samples from 163 herds from all States in Australia, there was some evidence of reactivity in only four samples from four herds on the enzyme-linked immunosorbent assay. Further testing using the indirect immunofluorescence antibody assay according to the study protocol demonstrated that the reactions were not due to the presence of specific porcine reproductive and respiratory syndrome virus antibodies in the sera.
Conclusion The results of this study support the view that Australian pigs are free of porcine reproductive and respiratory syndrome virus. 相似文献
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Chun-Chieh Yang Shiv O. Prasher Shaoli Wang Seung Hyun Kim Chin S. Tan Craig Drury Ramanbhai M. Patel 《Agricultural Water Management》2007
DRAINMOD-N, a mathematical model to predict nitrate-N concentrations in surface runoff and drain outflows from subsurface-drained farmlands, has been tested against field data collected in southern Ontario. The data was collected in a corn field from 16 conventional drainage and subirrigation plots in Woodslee, Ontario, from 1992 to 1994. The model performance was evaluated by comparing the observed and simulated nitrate-N concentrations in surface runoff and drain outflows. A precise calculation of water-table depth is an essential prerequisite for a model to obtain a proper prediction of nitrate-N movement. For the simulation of water-table depth, the lowest root mean square error and the highest correlation coefficient of linear regression were 173 mm and 0.51 for the subirrigation plots; and 178 mm and 0.84 for the subsurface drainage plots. Therefore, the performance of DRAINMOD-N for soil hydrologic simulations was satisfactory and it could be used for assessing nitrogen fate and transport. For the simulation of nitrate-N losses in the subirrigation plots, the lowest root mean square error and the highest correlation coefficient of linear regression were 0.74 kg/ha and 0.98 for surface runoff; and 6.53 kg/ha and 0.91 for drain outflow. For the simulation in the subsurface drainage plots, the lowest root mean square error and the highest correlation coefficient of linear regression were 0.70 kg/ha and 0.96 for surface runoff; and 6.91 kg/ha and 0.92 for drain outflow. The results show that DRAINMOD-N can perform satisfactory simulation of soil hydrology and nitrate-N losses in surface runoff under various water-table management practices. The model can, therefore, be used to evaluate different water pollution scenarios and help in the development and testing of various pollution control strategies for fields in cold weather such as that in southern Canada. 相似文献
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RE Hickson WJ Anderson PR Kenyon N Lopez-Villalobos ST Morris 《New Zealand veterinary journal》2013,61(4):176-183
AIM: To obtain an estimate of the incidence of assistance at calving in primiparous (first-calving) beef heifers and the prevalence of breeding heifers at 15 months of age in New Zealand in 2006,and to identify factors contributing to farmers' decisions regarding breeding strategies for heifers, using a survey of beef cattle farmers. METHODS: A questionnaire was sent to farmers listed in a Massey University database and to members of selected breed societies, as well as published in an industry newspaper; 331 valid responses were received. Information was gathered on the age and number of primiparous heifers, number of heifers assisted, and the importance of various reasons for and against breeding heifers at 15 months of age. Respondents were also required to outline the criteria used for selecting bulls to join with heifers, and the strategies used to manage dystocia in primiparous heifers. RESULTS: Sixty-five (95% CI=58–71)% of respondents had only 2-year-old primiparous heifers in 2006, whilst a further 11 (95% CI=8–16)% had both 2- and 3-year-old primiparous heifers. The mean reported incidence of assisted calving was 7.0 (95% CI=6.4–7.5)% for 2-year-old primiparous heifers and 1.7 (95% CI=1.2–2.2)% for 3-year-old primiparous heifers. The reported incidence of assistance at calving within individual herdsranged from 0 to 100% for 2-year-old heifers. Respondents with bull-breeding herds most commonly observed their primiparous 2-year-old heifers twice daily, whilst respondents with commercial herds most commonly observed them once daily during calving. The most important reason for breeding heifers at 15 months of age was “increased profit”, whereas the most important reason for not breeding them at that age was “concern about rebreeding performance of 2-year-old heifers”. Estimated breeding value (EBV) for birth weight was the factor considered most frequently when selecting bulls to join with maiden heifers; age of bull and body shape of bull were the next most frequently considered factors. Selection of an appropriate bull was the most common strategy used to manage dystocia in 2-year-old beef heifers. CONCLUSIONS: The majority of respondents calved their heifers at 2 years of age, and “increased profit” was the primary motivator. Concern about the rebreeding performance of 2-year-old heifers was the most important reason among the remainder of respondents for not breeding heifers at 15 months of age. Dystocia in 2-year-old heifers was “not a problem” or “a minorproblem” in most herds, but there was much variation amongst herds. 相似文献
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Jim Miller Bruce Beasley Craig Drury Frank Larney Xiying Hao 《Compost science & utilization》2016,24(1):54-60
The influence of annual applications of composted (CM) or stockpiled (SM) beef feedlot manure with straw (ST) or wood-chip (WD) bedding on cation exchange capacity (CEC) of a clay loam soil in southern Alberta was examined after 1, 8, and 15 years. The hypotheses in our study were that soil CEC should be greater for amended than unamended soils; manure type and bedding should have no effect on soil CEC; and soil CEC should increase with greater manure application rate. After fifteen applications, the CEC was significantly greater for amended than unamended soils. Manure type had no significant (P > 0.05) effect on soil CEC after fifteen applications, and the mean soil CEC was 5% greater for WD than ST. Mean CEC was significantly greater by 7 to 12% for the 77 than the 13 and the 39 Mg ha?1 rates after fifteen applications. The soil CEC was increased by 0.061 cmolc kg?1 for a unit increase in application rate (Mg dry wt. ha?1 yr?1), and 96% of the variation in CEC for amended soils could be explained by application rate. Overall, bedding, rate, or adjusting both bedding type and manure rate (but not manure type), may be possible practices for feedlot producers to manage soil CEC. 相似文献
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