Antioxidant and antiproliferative activities of heated sterilized pepsin hydrolysate derived from half-fin anchovy (Setipinna taty)

In this paper we studied the antioxidant and antiproliferative activities of the heated pepsin hydrolysate from a marine fish half-fin anchovy (HAHp-H). Furthermore, we compared the chemical profiles including the amino acid composition, the browning intensity, the IR and UV-visible spectra, and the molecular weight distribution between the half-fin anchovy pepsin hydrolysate (HAHp) and HAHp-H. Results showed that heat sterilization on HAHp improved the 1,1-diphenyl-2-picryl-hydrazil (DPPH) radical-scavenging activity and reducing power. In addition, the antiproliferative activities were all increased for HAHp-H on DU-145 human prostate cancer cell line, 1299 human lung cancer cell line and 109 human esophagus cancer cell line. The contents of free amino acid and reducing sugar of HAHp-H were decreased (P < 0.05). However, hydrophobic amino acid residues and the browning intensity of HAHp-H were increased. FT-IR spectroscopy indicated that amide I and amide III bands of HAHp-H were slightly modified, whereas band intensity of amide II was reduced dramatically. Thermal sterilization resulted in the increased fractions of HAHp-H with molecular weight of 3000-5000 Da and below 500 Da. The enhanced antioxidant and antiproliferative activities of HAHp-H might be attributed to the Maillard reaction.     

鲁中地区玉米高产栽培技术

利用鲁中地区的光照、气候、土壤等自然资源,选用适合当地熟期的高产、高效、优质玉米杂交品种,因地制宜地采用合理的栽培技术、精耕细作、科学施肥管理并创建高产群体,是获得玉米高产的保证.     

不同培肥方式对茶园土壤团聚体中有机碳和全氮分布的影响

通过长期定位试验,研究不施肥（CK）、100%化肥（CF）和50%化肥+50%有机肥+豆科植物（IF）等3种处理对茶园黄红壤团聚体及其有机碳（TOC）和全氮（TN）分布的影响。结果表明,各处理均以≥2βmm水稳性团聚体占优势,其质量分数高达54.95%~66.97%;同时,该级水稳性团聚体中TOC、TN储量分别是相应全土总量的33.31%~45.45%和33.76%~46.60%。其中,不同粒径水稳性团聚体TOC和TN的贡献率在IF和CF处理中的大小排序为（≥2βmm）>（<0.5~0.25βmm）>（<1~0.5βmm）>（<2~1βmm）>（<0.25~0.106βmm）,而在CK中为（≥2 mm）>（<1~0.5βmm）>（<0.5~0.25βmm）>（<2~1βmm）>（<0.25~0.106βmm）。与CK相比,IF处理可显著（P<0.05）提升≥2βmm水稳性团聚体的含量和平均重量直径,增加该粒径水稳性大团聚体中TOC和TN的含量及储量。而且,≥2βmm水稳性团聚体含量与全土TOC、TN含量均呈极显著（P<0.01）的正相关。但是,IF处理土壤不同粒径团聚体的C:N比值一般比CK低。综上表明,处理IF不仅能增加土壤及其水稳性团聚体中TOC和TN的含量及储量,而且提高水稳性团聚体的稳定性,是茶园黄红壤固碳供氮的合理培肥模式。     

粘性土坡稳定的可靠性分析初探

本文基于概率理论对某一粘性土坡稳定性进行可靠性分析，给出了计算土坡稳定的可靠性指标及效概率的方法和评价和可靠的标准。该方法与传统的采用单一的安全系数表示土皮稳定的方法相比，能够表明土坡稳定直实的安全度和失效的可能程度，可靠性分析方法在一定程度上弥补了传统的定值论方法的不足之处。     

吉林省食品安全监测检验机制存在的问题与对策

近几年来,吉林省食品安全危机事件频发,要增强对食品安全的控制能力,做到对食品安全危机有效预警,政府部门应该建立食品安全监测检验机制,及时掌握食品安全的监测数据。本文通过分析吉林省食品安全监测检验机制的现状及存在的问题,阐述了完善我省食品安全监测检验机制的对策。     

套袋对毛花猕猴桃果实外观和内在品质的影响

为探讨套袋对毛花猕猴桃果实品质的影响,以不套袋为对照,分别采用生产上常用的白色单层袋和外黄内黑单层袋在花后30 d对毛花猕猴桃果实进行套袋,果实生理成熟期取样测定外观和内在品质的各指标.结果 表明,套袋处理均能有效改善果实外观品质,提高果实硬度;白色单层袋显著提高了果实抗坏血酸、可溶性糖和可滴定酸含量,外黄内黑单层袋显...     

Ozone Disinfection Test in Chicken House

[Objective] The paper was to verify the disinfection effect of ozone on livestock and poultry houses.[Method] The empty chicken house was disinfected by ozone with the help of ozone disinfector.[Result] As the ozone disinfector was worked for 60 min,the average sterilizing rate of air was 85.33%;the average sterilizing rate on the surface of chicken coop was 86.77%;and the average sterilizing rate of wall was 89.13%.The disinfection effect was obvious.[Conclusion] The results will provide references for promotion and application of ozone disinfector in breeding in-dustry.     

Bioinformatics analysis of tyrosinase-related protein 1 gene (<Emphasis Type=Italic>TYRP1</Emphasis>) from different species

As one member of the tyrosinase-related family directly involved in the production of melanin, TYRP1 is involved in not only melanogenesis but also prevention of melanocyte death, stabilizing tyrosinase and helping determine the shape of melanosomes, etc. Multi-species sequence comparisons showed that there were two evolutionally conserved non-coding regions (from −1306 to −733 and from −642 to −515 according to AL138753) upstream of translational initiation sites, representing putative regulatory regions subject to subsequent experimental tests. Coding sequence length variation and genetic diversity analysis showed that Felis catus, Homo sapiens and Canis familiaris had more genetic diversities than the other species for TYRP1, especially Felis catus that could be a better choice for studying the TYRP1-associated genetic basis underlying the color diversity. As a 75 kDa type-1 transmembrane glycoprotein, mature TYRP1 possesses about 17 kDa modifying components, whose function predominantly depends on the existing glycosyl-groups and the Cu components. In addition, the mutated amino acids within species and the highly conserved amino acids among species were listed in our paper.     

Mulching mode and planting density affect canopy interception loss of rainfall and water use efficiency of dryland maize on the Loess Plateau of China

High and efficient use of limited rainwater resources is of crucial importance for the crop production in arid and semi-arid areas. To investigate the effects of different soil and crop management practices(i.e., mulching mode treatments: flat cultivation with non-mulching, flat cultivation with straw mulching, plastic-covered ridge with bare furrow and plastic-covered ridge with straw-covered furrow; and planting density treatments: low planting density of 45,000 plants/hm~2, medium planting density of 67,500 plants/hm~2 and high planting density of 90,000 plants/hm~2) on rainfall partitioning by dryland maize canopy, especially the resulted net rainfall input beneath the maize canopy, we measured the gross rainfall, throughfall and stemflow at different growth stages of dryland maize in 2015 and 2016 on the Loess Plateau of China. The canopy interception loss was estimated by the water balance method. Soil water storage, leaf area index, grain yield(as well as it components) and water use efficiency of dryland maize were measured or calculated. Results showed that the cumulative throughfall, cumulative stemflow and cumulative canopy interception loss during the whole growing season accounted for 42.3%–77.5%, 15.1%–36.3% and 7.4%–21.4% of the total gross rainfall under different treatments, respectively. Soil mulching could promote the growth and development of dryland maize and enhance the capability of stemflow production and canopy interception loss, thereby increasing the relative stemflow and relative canopy interception loss and reducing the relative throughfall. The relative stemflow and relative canopy interception loss generally increased with increasing planting density, while the relative throughfall decreased with increasing planting density. During the two experimental years, mulching mode had no significant influence on net rainfall due to the compensation between throughfall and stemflow, whereas planting density significantly affected net rainfall. The highest grain yield and water use efficiency of dryland maize were obtained under the combination of medium planting density of 67,500 plants/hm~2 and mulching mode of plastic-covered ridge with straw-covered furrow. Soil mulching can reduce soil evaporation and retain more soil water for dryland maize without reducing the net rainfall input beneath the maize canopy, which may alleviate the contradiction between high soil water consumption and insufficient rainfall input of the soil. In conclusion, the application of medium planting density(67,500 plants/hm~2) under plastic-covered ridge with bare furrow is recommended for increasing dryland maize production on the Loess Plateau of China.     

壳聚糖对14～70日龄扬州鹅生长性能、屠宰性能、脏器指数及血清生化指标的影响

本试验旨在研究饲粮中添加不同水平壳聚糖对14~70日龄扬州鹅生长性能、屠宰性能、脏器指数及血清生化指标的影响,以确定鹅饲粮中壳聚糖适宜添加水平。选取360只14日龄健康、体重相近的扬州鹅公鹅,随机分为5个组,每组6个重复,每个重复12只鹅。对照组饲喂基础饲粮,试验组分别饲喂在基础饲粮中添加250、500、1 000和2 000 mg/kg壳聚糖的试验饲粮。试验期56 d。结果表明,与对照组相比:1)饲粮添加500 mg/kg壳聚糖显著提高了42、56和70日龄鹅体重及14~70日龄平均日采食量、平均日增重(P0.05),显著降低了14~70日龄料重比(P0.05)。2)饲粮添加250、500、1 000和2 000 mg/kg壳聚糖显著降低了鹅腹脂率(P0.05)。3)饲粮添加500 mg/kg壳聚糖显著提高了鹅心脏指数、肝脏指数、脾脏指数、空肠指数、回肠指数和盲肠指数(P0.05)。4)饲粮添加500 mg/kg壳聚糖显著提高了鹅血清球蛋白、葡萄糖含量(P0.05),显著降低了血清碱性磷酸酶活性(P0.05);饲粮添加250、500、1 000 mg/kg壳聚糖显著降低了血清中甘油三酯、总胆固醇含量(P0.05)。由此可知,饲粮中添加壳聚糖可以改善扬州鹅生长性能,对其屠宰性能、脏器指数和血清生化指标有一定影响。在生产实践中,扬州鹅饲粮中壳聚糖添加500 mg/kg效果最佳。