苹果绵蚜综合防治试验

通过根施、灌根、涂环、虫疤涂泥、喷雾、综合防治等试验,明确了苹果绵蚜防治的关键时期,确定了防效好的药剂,总结出了加强检疫、根施药剂、树干刮涂、树上喷药、全程保护利用自然天敌等一系列综合治理措施,有效地控制了苹果绵蚜的危害。     

酵母蛋白饲料培养条件的研究

对啤酒酵母进行发酵试验,结果表明:该酵母最优的培养条件是麦芽汁培养基稀释倍数2.5,培养时间20h,蔗糖添加量10Brix,尿素添加量0.02%,且酵母菌耐酸耐铜的性能好。     

犬胚胎移植手术路径研究性试验

犬胚胎移植手术路径的确定是移植能否成功的关键。我们选择了12例自然发情的母犬，分为供体组和受体组，每组6头。通过生物检测镜确定排卵时间，用腹腔镜观察卵巢黄体发育情况，同时于交配后第9、11、13天剖腹探查，确定冲卵时间。结果于交配后第11天冲洗供体犬左侧子宫角，保留右侧子宫角的两例犬受孕，移植给2头受体犬左侧子宫角。     

金龟子绿僵菌固态培养生物变量优化研究

金龟子绿僵菌属于真菌类生物杀虫剂,本文研究了接种量、种龄、菌种代数等生物因素对金龟子绿缰菌生长及产孢量的影响,并对液—固两步发酵工艺进行了研究。经过优化,得到金龟子绿僵菌产孢的最佳生物参数是:接种量为2.5g/100g、种龄为5-6天;采用固体二代种,培养6天,孢子产量可达1.531×10~(10)孢子/g干培养基。     

The effect of maternal obesity on fatty acid transporter expression and lipid metabolism in the full‐term placenta of lean breed swine

This study was conducted to evaluate the influence of back‐fat thickness (BF), at mating of sows, on the maternal and newborn circulating lipids, expression of placental fatty acids (FA) transporters and lipid accumulation in placenta. Full‐term placentas were obtained by vaginal delivery from BFI (9–14 mm; n = 37), BFII (15–19 mm; n = 43) and BFIII (20–27 mm; n = 38) sows according to BF at mating, and frozen placental sections were analysed for fat accumulation. Blood samples were collected from the sows of day 105 pregnancy and from cord blood at delivery. mRNA and protein expression levels were evaluated with real‐time RT‐PCR and Western blotting. Our results demonstrated that BFII females had significantly increased litter weight and placental efficiency, decreased maternal triglyceride (TG) and non‐esterified fatty acids (NEFA) levels, decreased maternal IL‐6, TNFα and leptin levels compared to BFIII females (p < .05). BFIII sows were associated with significantly decreased newborn TG levels, increased newborn glucose, IL‐6 and TNFα levels compared to BFI or BFII sows (p < .05). BFI and BFII females had significantly decreased placental TG, NEFA and cholesterol (CHOL) contents compared to BFIII females (p < .05). Moreover, decreased CD36, FATP1, FABP4, and FABP1 mRNA and protein and FATP4 protein expression, and increased LPL activity were also observed in BFIII group compared with BFII group (p < .05). PPARγ mRNA and protein and lipogenic genes such as SREBP‐1c, ACSL1, ACCα, FAS and SCD mRNA expression were downregulated or upregulated, respectively, in the placentas of BFIII sows compared to BFI or BFII sows (p < .05). Overall, this study demonstrated that there is no advantage, in terms of litter live size, litter weight and placental FA transport and metabolism, in performing the mating of sows with BF>19 mm.     

一起销售劣兽药案的查处与思考

云南省德宏州动物卫生监督所通过抽检发现,辖区内某兽药经营服务部涉嫌销售劣兽药。经该县农业局立案调查,认定当事人违法事实清楚,证据充分,根据《兽药管理条例》规定,依法给予了行政处罚。本文重点介绍了案件来源、调查过程、处理情况等执法内容。分析了案件定性、执法主体适格、自由裁量权应用、保护当事人合法权益等问题,以期为同类案件查处提供参考。     

湄江翠片加工工艺中香气与色泽的相关性初探

详细介绍了湄江翠片茶各种工艺组合,并根据制茶原理,分析了制茶因子对湄江翠片的品质影响,通过分析对比,找出了湄江翠片比较合理的工艺组合。     

浅谈住宅工程初装饰

为适应市场经济的发展、满足广大住户对住宅二次装饰的需要,对住宅工程进行初装饰已势在必行.本文探讨了住宅工程初装饰的具体做法和质量葆证措施.     

抗生素对泡桐丛枝病植原体和发病相关蛋白质的影响

泡桐(Paulownia sp.)是我国重要的速生用材和绿化树种;然而,丛枝病的发生给我国的泡桐生产带来了巨大的损失.     

杉木不同世代不同类型种子园遗传改良增益研究

以浙江省17个无性系种子园为研究对象,连续2年采种育苗造林,进行2次4地点5区组的种子园子代测定试验,测定树高、胸径、材积、冠幅、侧枝等8个性状.结果表明:从未经遗传改良普通林分种子到1代、1.5代、2代种子园,子代生长增益是逐代增长的,但世代之间增幅明显下降.优良双系种子园子代生长增益最大,优良种源种子园次之.1代、1.5代和2代种子园,种源种子园,双系种子园的子代生长,在6年生时,树高生长现实增益分别为4.35%、6.04%、7.88%、12.02%和12.02%,遗传增益依次为3.90%、5.41%、7.06%、10.77%和10.77%;胸径现实增益为7.60%、9.68%、12.08%、15.05%和19.70%,遗传增益为7.07%、9.00%、11.23%、14.00%和18.32%;材积现实增益为20.65%、27.47%、32.14%、39.93%和46.14%,遗传增益为16.97%、22.58%、26.42%、32.82%和37.93%.为持续获得高增长的遗传改良效果,在高世代种子园建设中,应充分发掘和优先利用优良种源的优质种质资源;在提升育种群体遗传水平,保持遗传基础多样性的同时,建立双系种子园,实现工业原料林用种的品种化,应是今后的一个重要发展方向.