Effects of breed and harvest age on feed intake, growth, carcass traits, blood metabolites, and lipogenic gene expression in Boer and Kiko goats

The objectives of this experiment were to determine the effects of 2 different breeds (BR), Boer and Kiko, and 4 post-weaning harvest ages (HA; Days 0, 29, 56, and 85) on growth, carcass traits, blood metabolites, and lipogenic gene expression. Forty-eight goat (Capra hircus) kids (BW = 23.9 ± 1.50 kg; 3 to 4 mo) were used in a 2 × 4 factorial arrangement of treatments. Goats were stratified by BW within BR and randomly assigned to 4 HA. Kids were born between March 15 and April 7 to purebred does, and were represented by at least 3 purebred sires within each BR. They were fed a grain/hay (80:20) diet once per day. At designated HA, randomly pre-assigned goats (n = 6) from each BR were transported to the Meat Science Lab at Mississippi State University, Starkville, MS, and were harvested. There were no interactions (P > 0.10) between BR and HA. Boer tended (P = 0.08) to have greater initial BW, final BW (P = 0.05), and G/F ratio (P = 0.05). Although the 80:20 grain/hay diet was reinforced by adjusting DMI, both BR had similar total DMI, Boer kept that ratio, while Kiko consumed more (P = 0.001) hay (70:30, grain/hay) and had more (P = 0.001) DMI when expressed as g/kg BW. Boer tended to have greater transportation shrink (P = 0.07), HCW (P = 0.08), and cold carcass weights (CCW; P = 0.08), with greater (P = 0.001) carcass fat. No differences (P > 0.10) were observed in carcass shrink, dressing percentage, 12th rib fat thickness, and LM area between the 2 BR. When expressed as percentage empty BW, carcass bone was similar (P = 0.25), whereas muscle percentage (P = 0.02) was greater for Kiko and fat percentage was greater (P = 0.001) for Boer. Fat as a percentage of CCW remained relatively similar (P > 0.10) for both BR for the 2nd and 3rd HA. Differences were more evident (P = 0.01) at the 4th HA. Boer reached targeted harvest weight (29 kg) at the 3rd HA, while fat deposition continued (P = 0.01) during the 4th HA. Breed had no effect (P > 0.10) on meat color (L*, a*, b*) but HA affected (P = 0.001) all color values. Boer had similar 3-hydroxyl-3-methylglutaryl-CoA synthase mRNA abundance, but was greater (P < 0.03) in acetyl CoA carboxylase compared with Kiko. There was no difference (P = 0.52) in total serum fatty acids (FA, mg/mL) between the 2 BR. As animals aged, their total serum FA increased (P < 0.05) and changed to an undesirable profile. Kiko had a greater (P = 0.02) percentage of muscle and less (P = 0.001) fat in the carcass. We concluded that different BR might need different harvest endpoints and feed input according to consumer acceptability.     

Distribution and prevalence of footrot in Bhutan

The first cases of footrot in Bhutan were reported in sheep in 1990 at the National Sheep Breeding Centre (NSBC), which supplies breeding animals to village sheep flocks throughout Bhutan. Despite the presence of footrot at the Centre the distribution of apparently disease-free sheep continued. Cases of footrot were reported in village flocks soon after the disease was diagnosed at NSBC. A national survey was designed to establish the distribution and prevalence of footrot in Bhutan. This detected footrot in 19/94 village sheep flocks surveyed. The 19 affected flocks were distributed among nine different administrative districts whereas the villages selected were in 13 of a total of 16 sheep growing districts. The highest within-flock prevalences were among the seven flocks sampled in Bumthang district (mean 20.4%). The prevalence of the disease within flocks was generally much lower in other affected districts and in three districts a single affected animal was identified in the sample of 14 sheep examined in each village. Nationally, footrot prevalence was estimated to be 3.1% (95% CI 2.16-4.04%). There was a positive association between the receipt of animals from NSBC and the presence of footrot. The prevalence of the disease was higher in flocks with a migratory system of management than in those using a sedentary system. The relative risk of there being footrot in a migratory flock was nine-times higher than in a non-migratory flock. Only one strain of Dichelobacter nodosus (serogroup B) was identified among the 234 isolates obtained from the 19 affected flocks. Sheep with footrot healed quickly when treated with a vaccine made from this strain.     

Potential of Metarhizium anisopliae and Beauveria bassiana in the control of tea termite Microtermes obesi Holmgren in vitro and under field conditions

Two strains of Metarhizium anisopliae (Metschnikoff) Sorokin and two strains of Beauveria bassiana (Ballsamo) Vuillemin were evaluated for their potential use as biological control agents against the tea termite Microtermes obesi Holmgren. The strains were screened for relative pathogenicity, and the median lethal time eliciting 50% mortality (LT₅₀) was calculated. In general, the M. anisopliae strains were more virulent with lower LT₅₀ values than B. bassiana strains. The LT₅₀ values ranged from 1.6 to 3.7 days. The median lethal concentrations (LC₅₀) of all the isolates were also determined. The M. anisopliae strains had low LC₅₀ values compared to B. bassiana strains. The LC₅₀ values ranged from 35 to 140 conidia per termite. The strains were also tested for survival under laboratory nest conditions. All the isolates exhibited the ability to grow, sporulate and produce an epizootic in treated nest material. Field applications of the isolates also produced promising results.     

Factors associated with human-killing tigers in Chitwan National Park, Nepal

Human-killing is the ultimate expression of human-tiger conflict. It is a complex issue that needs to be addressed to maintain support for tiger (Panthera tigris) conservation in areas where human-killing is prevalent. This research was undertaken to investigate the ecological and sociological aspects of human-killing in the central lowlands of Nepal. We used 28 years of data from human-killing events in Chitwan National Park and the surrounding area to: (1) document the geographic distribution of human-killing incidents, (2) examine ecological variables associated with sites where humans were killed, (3) characterize human-killing tigers, and (4) identify human activities that make people vulnerable to attack. Finally, we use this information to recommend strategies to reduce human-tiger conflicts. Data on human-killing incidents and removal of human-killing tigers were obtained from veterinarian and Kathmandu Zoo records and by visiting the location of each kill with a victim’s family member or friend. Thirty-six tigers killed 88 people from 1979 to 2006. Most (66%) kills were made within 1 km of forest edge but equally in degraded and intact forests. An equal number of male and female tigers killed humans and 56% of tigers that were examined had physical deformities. The trend of human deaths increased significantly from an average of 1.2 (±1.2) persons per year prior to 1998 to 7.2 (±6.9) per year from 1998 to 2006. This difference is due primarily to a ten fold increase in killing in the buffer zone since 1998 because of forest restoration. Nearly half the people killed were grass/fodder collectors. Local participation in tiger management and conservation is essential to mitigate human-tiger conflicts. We recommend that villagers be recruited to help radio collar and monitor potentially dangerous tigers, participate in long term tiger monitoring, and attend a tiger conservation awareness program focused on tiger behavior and avoidance of conflict.     

Genetics of resistance in lettuce to races 1 and 2 of <Emphasis Type="Italic">Verticillium dahliae</Emphasis> from different host species

Race 1 resistance against Verticillium dahliae in lettuce was originally shown in the cultivar La Brillante to be conditioned by a single dominant gene (Verticillium resistance 1, Vr1). Multiple, morphologically diverse sources of germplasm have been identified as resistant to race 1. In this study, allelism tests indicated that resistance in these different lettuce cultivars is closely linked or allelic to the Vr1 gene. The Vr1 gene is defeated by race 2 isolates of V. dahliae. Only partial resistance to race 2 isolates is available in a few plant introductions (PIs). Greenhouse and field experiments conducted with these PIs demonstrated partial resistance to V. dahliae race 1 as well as race 2 isolates from lettuce. Cultivars resistant to race 1 and PIs with partial resistance to race 2 were challenged with several race 1 and 2 isolates originating from hosts other than lettuce. This indicated that cultivars resistant to race 1 and the breeding lines derived from them would also be resistant to race 1 isolates from other hosts; similarly, the partial resistance would be effective against race 1 and 2 isolates from hosts other than lettuce. Nevertheless, there were specific interactions that warrant further study. Although race 1 currently predominates in the major lettuce production area of the Salinas Valley, CA, breeding lettuce for resistance to V. dahliae should take both races into account.     

Insects – a mistake in God's creation? Tharu farmers' perception and knowledge of insects: A case study of Gobardiha Village Development Committee, Dang-Deukhuri, Nepal

Recent trends in agriculturalresearch and development emphasize the need forfarmer participation. Participation not onlymeans farmers' physical presence but also theuse of their knowledge and expertise.Understanding potentials and drawbacks of theirlocal knowledge system is a prerequisite forconstructive collaboration between farmers,scientists, and extension services.An ethnoentomological study, conducted in aTharu village in Nepal, documents farmers'qualitative and quantitative knowledge as wellas perceptions of insects and pest management,insect nomenclature and classification, andissues related to insect recognition and localbeliefs. The study offers a basis to improvepest management programs in terms of efficacyand acceptance. It demonstrates, for instance,that a concept of pests and beneficials isvirtually missing in traditional farmingcommunities and that the Tharu folkclassification profoundly differs from thescientific classification, but is not radicallydifferent from other folk entomologicalsystems. Insects belong to the taxa calledkiraa consisting of arthropods andnon-arthropods that interact with humans. Theyare classified in several overlappinghierarchies where locomotion and human impactplay major roles while morphological criteriaare almost irrelevant. Recognition ofkiraa, however, is dominated by agriculturalaspects followed by physiological-behavioral,ecological, and human-directed features.Morphological criteria play a minor role. Innomenclature, however, the insects' physicalappearance is more important than otherfeatures. The study further shows that male andfemale farmers have different perceptions ofkiraa.The insect-related knowledge system of theTharu has prevented farmers from using modernpesticides in the past. In the course ofmodernization, however, some aspects of theirknowledge system could become obsolete andprove disadvantageous to their livelihood andagro-ecosystems.