Activity of pulmonary intravascular macrophages in cats and dogs with and without adult Dirofilaria immitis

Pulmonary intravascular macrophages (PIMs), large (20-80 microm diameter) monocytes are present in sheep, pigs, and horses, but not in dogs, rats, rabbits, or primates. The present study evaluated the phagocytic activity of various organs in cats and dogs and determined the influence of Dirofilaria immitis infections on PIM activity. Live or dead adult heartworm (HW) was transplanted via jugular venotomy into cats and dogs. Cats (four per group) were allocated to five groups: surgical controls--no HW, dead HW for 1 week, live HW for 1 week, dead HW for 3 weeks, or live HW for 3 weeks. Radioactive technetium (Tc-99m, 1.2mCi in 0.3ml) sulfa-colloid was injected intravenously. All cats with HW were clinically asymptomatic and developed radiographic pulmonary parenchymal changes. No gross changes were visible at necropsy for cats with HW; inflammatory changes were less severe in cats with live HW. In cats with dead HW for 3 weeks, worms were present but folded, flattened, and located in distal pulmonary arteries. Uninfected control dogs and those with dead HW did not demonstrate any PIM activity. In control cats, lungs were the primary phagocytic organ after systemic IV colloid injection (72.5% of the total recovered radioactive dose). The lung and liver together represented over 95% of the recovered Tc-99m colloid in all cats. In each group of cats with HW, phagocytic activity of the lung was significantly less (p < 0.001) than the PIM activity of controls. Cats with dead HW at 1 week (50.1%) had a significant (p < 0.019) decrease in PIM activity compared with cats with dead HW at 3 weeks (59.5%). The PIM activity in cats with live HW was significantly decreased (p < 0.001) from that in groups with dead HW, but there was no significant difference between the two groups infected with live worms. There were no significant differences in recovery between any groups in pairwise analysis of the spleen, heart, skeletal muscle, kidney, bone marrow, or blood. Significant increases (p < 0.001) in liver activity for each group inversely reflected the decreased lung activity; consistent with increased hepatic uptake of Tc colloid "escaping" a relatively suppressed lung macrophage system. Transmission electron microscopy confirmed PIM glycocalyx changes and vacuolization, moderate Type 1 cell damage and Type II cell hypertrophy in cats with dead HW. There was no evidence of PIM death. The significant decrease in PIM activity in groups with dead HW and a greater decrease in groups with live HW are consistent with a down-regulation of PIM function in cats with live HW.     

Greenhouse gas emissions from pastoral based dairying systems: The effect of uncertainty and management change under two contrasting production systems

A previously developed model, the Pastoral Milk Emission Model (PME Model) was used to simulate both on-farm and off-farm greenhouse gas (GHG) e`missions (consisting of methane (CH₄), nitrous oxide (N₂O) and carbon dioxide (CO₂)) under two contrasting pastoral based milk production systems. There were two primary objectives to this study. Firstly within the production systems studied, to identify the effect simple management changes may have on GHG emissions and farm profitability so that financially viable whole farm GHG mitigation strategies could be developed. The potential effect these management changes could have on national GHG emissions was quantified. The second objective was to determine what effect the characteristics of the production system may have on the uncertainty surrounding the emission of GHGs.The two contrasting production systems were the standard production procedures recommended for Kilmaley, (County Clare, Ireland) and Moorepark (County Cork, Ireland), with grazing seasons of 149 and 250 days per year respectively. The management scenarios tested were changes in pasture quality, pasture utilisation, N application rates, silage quality, level of concentrate supplementation and calving date.Stochastic budgeting revealed that emissions of GHGs were greater at Kilmaley than Moorepark whatever the level of risk/uncertainty. Simple farming system changes, regardless of location, generally resulted in small changes in both on-farm and total GHG emissions (between—5.8 and + 5.1%). However silage quality demonstrated a site specific interaction. Tailor designed whole farm GHG mitigation strategies were developed by combining all identified management changes, which simultaneously reduced GHG emissions and increased farm profitability. Relative to the standard production systems at Moorepark and Kilmaley, on-farm and total GHG emissions were 6.65% and 8.42% and 11.40% and 11.64% respectively. Farm profitability increased at both sites. Projecting these potential reductions onto the national dairy herd revealed that significant reductions in GHG emissions are possible. Relative to 2005 national emissions the implementation of the standard systems would achieve a reduction of 3.46%, this would increase to 4.04% for the tailor designed strategies. Additional reductions in GHG emissions would be achieved through a lowering of inputs within the farm supply chain.Sensitivity analysis revealed differences between systems with regards to source strength, however regardless of the system, source strength was always greatest for enteric CH₄, confirmation that considerable efforts should be made to control this GHG source. However differences do exist between systems which suggests that national mitigation strategies must consider regional production systems if GHG reductions are to be maximised. This work has also highlighted the need for more data on indirect GHG emissions derived from leached N.     

Left ventricular outflow tract to left atrial fistula associated with endocarditis in a dog

A one-year-old, intact male, 28-kg, mixed-breed dog developed neurological episodes consistent with emboli. An acquired III/VI holosystolic heart murmur was ausculted in the mitral area, and valvular endocarditis with pulmonic and aortic insufficiency were noted at echocardiographic examination. An abnormal communication (i.e., fistula) between the left ventricular outflow tract (LVOT) and the left atrium adjacent to the mitral valve annulus was noted with Doppler imaging and confirmed with angiography. Infective valvular endocarditis was confirmed based on two of three blood cultures being positive for Staphylococcus intermedius. In humans, a sequela to infective endocarditis of the aortic or mitral valve, or both, is rupture of the mitral-aortic intervalvular fibrosa, resulting in a communication between the LVOT and the left atrium. This is the first report of this sequela in the dog.     

Routine inertia and reactionary response in animal health best practice

Animal health is a key factor affecting the economic efficiency of the dairy industry. Improvements in animal health are also of relevance to society more broadly, given important implications for animal welfare, food safety and quality. Although the economic gains of best practice with regard to animal health have been well documented, many farmers are not adopting optimal herd management techniques. This paper utilises nationally representative farm-level data from Ireland for 2013 to identify drivers and barriers to the adoption of best practice with regard to on-farm mastitis management. Exploratory factor analysis is used to derive measures of farmers’ attitudes towards animal health and mastitis and econometric techniques are employed to empirically assess the influence of these on the uptake of beneficial herd health management practices. A number of focus groups were also undertaken to complement the analysis. This paper concludes that farmers’ attitudes towards animal health are not a key driver in the uptake of best practice, although perceived disease risk is of relevance. A number of interesting issues arise in identifying barriers to the uptake of best practice, these include the possibility of routine inertia, i.e., farmers do not deviate from the routine developed around mastitis prevention until there is an indication of infection, as well as constraints around the availability of labour and time. Farmer behaviour with respect to mastitis management can thus be considered as reactionary as opposed to precautionary. This research highlights the valuable role of the extension agent but concludes that engagement around knowledge transfer and technology adoption is particularly complex.     

Developing Acute-to-chronic Toxicity Ratios for Lead, Cadmium, and Zinc using Rainbow Trout, a Mayfly, and a Midge

In order to estimate acute-to-chronic toxicity ratios (ACRs) relevant to a coldwater stream community, we exposed rainbow trout (Oncorhynchus mykiss) to cadmium (Cd), lead (Pb), and zinc (Zn) in 96-h acute and 60+ day early-life stage (ELS) exposures. We also tested the acute and sublethal responses of a mayfly (Baetis tricaudatus) and a midge (Chironomus dilutus, formerly C. tentans) with Pb. We examine the statistical interpretation of test endpoints and the acute-to-chronic ratio concept. Increasing the number of control replicates by 2 to 3× decreased the minimum detectable differences by almost half. Pb ACR estimates mostly increased with increasing acute resistance of the organisms (rainbow trout ACRs <≈ mayfly < Chironomus). The choice of test endpoint and statistical analysis influenced ACR estimates by up to a factor of four. When calculated using the geometric means of the no- and lowest-observed effect concentrations, ACRs with rainbow trout and Cd were 0.6 and 0.95; Zn about 1.0; and for Pb 3.3 and 11. The comparable Pb ACRs for the mayfly and Chironomus were 5.2 and 51 respectively. Our rainbow trout ACRs with Pb were about 5–20× lower than earlier reports with salmonids. We suggest discounting previous ACR results that used larger and older fish in their acute tests.     

Comparison of type and depth of lysimeter for measuring the leaching losses of nitrogen under urine patches

Abstract. A study of the leaching losses of nitrate under urine patches in irrigated and non-irrigated dairy pastures in the South East of South Australia was undertaken with repacked and monolith lysimeters 1 m deep, and with monolith lysimeters 150, 300 and 450 mm deep. The aim was to quantify differences in measurements of drainage and nitrogen fluxes for these different lysimeters. Drainage of water and N flux were found to vary significantly between types and depths of lysimeters. Drainage volumes in repacked lysimeters were 78% and 33% more than in monolith lysimeters in irrigated and non-irrigated paddocks, and N fluxes were 5 and 3 times higher in repacked lysimeters respectively. The results indicate that lysimeter estimates of recharge rates and N fluxes to water tables are best determined by leaching studies which are longer term, and use deep monolith lysimeters. Shorter term studies and the use of shallow or repacked lysimeters have potential to distort conclusions.     

Whole farm analysis of automatic section control for agricultural machinery

Automatic section control was analyzed in a whole farm decision-making framework when implemented on an agricultural sprayer and/or planter. In addition, various field types and navigational scenarios were examined to determine their impact on profitability. It was determined that automatic section control increased net returns under all scenarios; up to $36/ha. This investigation highlighted the importance of considering field size in addition to field shape as well as initial navigational scenarios when determining the profitability of automatic section control.     

High-resolution computed tomography bronchial lumen to pulmonary artery diameter ratio in anesthetized ventilated cats with normal lungs

High-resolution computed tomography (CT) is the preferred noninvasive tool for diagnosing bronchiectasis in people. A criterion for evaluating dilation of the bronchus is the bronchial lumen to pulmonary artery diameter (bronchoarterial ratio [BA ratio]). A ratio of > 1.0 in humans or > 2.0 in dogs has been suggested as a threshold for identifying bronchiectasis. The purpose of this study was to establish the BA ratio in normal cats. Fourteen specific pathogen-free cats were selected for analysis of thoracic CT images. The BA ratios of the lobar bronchi of the left cranial (cranial and caudal parts), right cranial, right middle, left caudal, and right caudal lung lobes were measured. The mean of the mean BA ratio of all lung lobes was 0.71 +/- 0.05. Individual BA ratios ranged from 0.5 to 1.11. Comparing individual lobes for each cat, there was no significant difference (P = 0.145) in mean BA ratio between lung lobes. A mean BA ratio for these normal cats was 0.71 +/- 0.1, which suggests an upper cut-off normal value > 0.91 (mean +/- 2 standard deviations) between normal and abnormal cats.