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Jeanne Dijkstra 《European journal of plant pathology / European Foundation for Plant Pathology》1969,75(6):321-328
A correlation between the amount of virus as determined by infectivity test and that determined by a serological-spectrophotometric method was established in leaf discs ofNicotiana tabacum White Burley which had floated on a solution of 6-azauracil (AzU) before and after inoculation with tobacco mosaic virus. More virus, determined in the two different ways, was present in the AzU-than in the water-treated leaf discs at 24 and 48 h after inoculation. Thereafter, the amount of virus was less than that in the control. The inhibitory effect of the pyrimidine analogue on the amount of infectivity in sap from AzU-treated discs proved to be dependent on the dilution of sap used. The higher the dilution the more inhibition occurred.In inoculated leaf discs ofNicotiana glutinosa the formation of local lesions in the AzU-treated series was strongly inhibited at 48 h after inoculation whereas at that time the amount of virus determined by the serological-spectrophotometric method was higher than that in the water-treated control ones. The latter mentioned stimulating effect disappeared later than 48 h after inoculation and gave way to inhibition.The infectivity of tobacco mosaic virus from AzU-treated leaf discs of White Burley tobacco was 23% of that from water-treated controls when undiluted clarified sap was used. 相似文献
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The situation in Thoroughbred breeding in relation to cytogenetics is considered and it is indicated where screening of horses will be useful in practice. The techniques involved are explained and karyotypes are illustrated. The present state of knowledge in horse cytogenetics is reviewed. The types of abnormality are described in relation to phenotype. Future promise is discussed in relation to the human field, concluding that with the refined methods now available a wider rangeof meaningful aberrations will be uncovered. 相似文献
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BRADLEY R. JACKMAN DVM MS GARY M. BAXTER VMD MS DIPIOMATE ACVS RICHARD E. DORAN DVM MS Dipiomate ACVS DOUGLAS ALLEN DVM MS Dipiomate ACVS ANDREW H. PARKS MA VET MB Dipiomate ACVS 《Veterinary surgery : VS》1993,22(4):285-288
The medical records of 57 horses that had palmar digital neurectomy performed between 1984 and 1990 were reviewed. Neurectomies were performed either by transection and elec-trocoagulation (47 horses) or by the guillotine technique (10 horses). Middle-aged geldings, Quarter Horses, and Thoroughbreds were significantly over-represented when compared with the hospital population. Horses used as hunter/jumpers also appeared to be over-represented. Complications occurred in 17 (34%) of the 50 horses for which follow-up information was obtained. Recurrence of heel pain was the most common complication (14 horses). Palpable painful neuromas were detected in three horses. One year after neurectomy, 74% of the horses were sound; this decreased to 63% after 2 years. 相似文献
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PAUL W. MORGAN DVM DVSc ALLEN G. BINNINGTON DVM MSc Diplomate ACVS CRAIG W. MILLER DVM MVSc Diplomate ACVS DALE A. SMITH DVM DVSc ANNE VALLIANT BSc JOHN F. PRESCOTT MA Vet MB PhD 《Veterinary surgery : VS》1994,23(6):494-502
This project compared the effects of hydrocolloid (HC) and hydrogel (HG) occlusive dressings and a polyethylene (PE) semi-occlusive dressing on the healing of acute full-thickness skin wounds on the forelimbs of 10 dogs. All treatments resulted in a similar degree of healing at postoperative days 4 and 7. No significant differences existed in the number of wounds that were more than 90% healed at postoperative day 28 between the group treated with the HG dressing and the group treated with the PE dressing. There were significantly fewer wounds more than 90% healed at postoperative day 28 in the group treated with the HC dressing. Wounds under the HG dressing had the largest mean percentage of contraction at postoperative days 21 and 28. Wounds under the HG dressing also had the largest contraction/re-epithelialization ratio (postoperative days 21 and 28) compared with wounds under the PE and HC dressings. Wounds under the PE dressing had a significantly higher mean percentage of re-epithelialization than wounds under both occlusive dressings on postoperative days 14, 21, and 28. Wounds under the two occlusive dressings had exuberant granulation tissue present more often than wounds under the PE dressing. The two occlusive dressings had significantly higher bacterial counts on wounds compared with wounds under the PE dressing; analysis of variance (ANOVA), P = .0008. Wounds under the HC dressing showed the poorest healing in all parameters. 相似文献
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LM Antunes MSc DVM JV Roughan BSc PhD & PA Flecknell MA Vet MB PhD DLAS Dip ECVA 《Veterinary anaesthesia and analgesia》2001,28(4):196-203
Objectives To assess a method for monitoring depth of anaesthesia using components of middle latency auditory evoked potential (AEP) waveforms during anaesthesia with fentanyl/fluanisone and midazolam. Study design Prospective observational study. Animals Five female Wistar rats weighing between 210 and 250 g. Methods Implanted electrodes were used to record AEPs in animals receiving five doses of anaesthetic. Recordings were made at 5 minutes post‐injection (deep anaesthesia; no pedal withdrawal response, PWR) and then at 25 minutes (light anaesthesia; strong PWR). Responses showed five characteristic peaks occurring at 11, 14, 23, 42 and 68 ms that were measured for latency of occurrence and peak amplitude. Results Auditory evoked potential peaks P14, N23 and P42 were increased significantly in latency with successive anaesthetic injections [avg. F(1,4) = 12.53, p < 0.001; avg. F(1,4) = 10.6, p < 0.001; avg. F(1,4) = 3.9, p = 0.02, respectively]. Peak N23 showed a significant reduction in latency during the 20 minute recovery period following both the first and second anaesthetic injections (t(3) = 7.52, p = 0.005; t(4) = 5.17, p = 0.007, respectively). Peak P42 occurred significantly earlier 20 minutes following the second anaesthetic injection (t(4) = 4.75, p = 0.009). The mean overall depth of anaesthesia assessed using PWR scores was significantly correlated with the mean latency of peak N23, such that as the strength of PWR increased, N23 occurred significantly earlier (r = ?0.99, p = 0.01). The amplitude difference between peaks N23 and P42 increased after the second and third drug administrations [avg. F(1,4) = 10.65, p = 0.031 and avg. F(1,4) = 11.24, p = 0.028, respectively]. Conclusion The characteristics of these peaks, and in particular latency of peak N23, may provide a useful tool for assessing depth of anaesthesia produced by this, and possibly other anaesthetic agents. 相似文献
29.
J. Dijkstra P. Grancini 《European journal of plant pathology / European Foundation for Plant Pathology》1960,66(5):295-300
Summary
Sorghum red stripe virus (S.R.S.V.) from Italy and sugar cane mosaic virus (S.C.M.V.) from Puerto Rico were investigated at Wageningen serologically and with the electron microscope.By means of the microprecipitation test (van Slogteren, 1955) a qualitative relationship was established between the two viruses (Table 1). Electron microscopical investigations showed that with regard to their length there was no difference between the two viruses (Fig. 1 and 2).Samenvatting Sorghum red stripe virus (S.R.S.V.), afkomstig uit Italië, en suikerrietmozaïekvirus (S.C.M.V.) uit Portorico zijn te Wageningen serologisch en elektronenmicroscopisch onderzocht. Met behulp van de microprecipitatietoets (van Slogteren, 1955) kon een kwalitatieve verwantschap tussen de beide virussen aangetoond worden (tabel 1). Elektronenmicroscopisch onderzoek wees uit, dat er wat betreft de lengte der virusdeeltjes geen verschil bestaat tussen de twee genoemde virussen (fig. 1 en 2). 相似文献
30.
Jeanne Dijkstra 《European journal of plant pathology / European Foundation for Plant Pathology》1968,74(4):101-105
Round or slightly oval inclusion bodies were found in low numbers in epidermal cells of spiked (expressing spike disease symptoms)
leaves. They could not be demonstrated in epidermal tissue of apparently healthy leaves. The intracellular inclusions stained
with phloxine and rose bengal. Their relationship to sandal spike virus is discussed. 相似文献