Antiplatelet and Anticoagulant Effects of Diterpenes Isolated from the Marine Alga,Dictyota menstrualis

Cardiovascular diseases represent a major cause of disability and death worldwide. Therapeutics are available, but they often have unsatisfactory results and may produce side effects. Alternative treatments based on the use of natural products have been extensively investigated, because of their low toxicity and side effects. Marine organisms are prime candidates for such products, as they are sources of numerous and complex substances with ecological and pharmacological effects. In this work, we investigated, through in vitro experiments, the effects of three diterpenes (pachydictyol A, isopachydictyol A and dichotomanol) from the Brazilian marine alga, Dictyota menstrualis, on platelet aggregation and plasma coagulation. Results showed that dichotomanol inhibited ADP- or collagen-induced aggregation of platelet-rich plasma (PRP), but failed to inhibit washed platelets (WP). In contrast, pachydictyol A and isopachydictyol A failed to inhibit the aggregation of PRP, but inhibited WP aggregation induced by collagen or thrombin. These diterpenes also inhibited coagulation analyzed by the prothrombin time and activated partial thromboplastin time and on commercial fibrinogen. Moreover, diterpenes inhibited the catalytic activity of thrombin. Theoretical studies using the Osiris Property Explorer software showed that diterpenes have low theoretical toxicity profiles and a drug-score similar to commercial anticoagulant drugs. In conclusion, these diterpenes are promising candidates for use in anticoagulant therapy, and this study also highlights the biotechnological potential of oceans and the importance of bioprospecting to develop medicines.     

Land Cover Transition in Northern Tanzania

Land conversion in sub‐Saharan Africa has profound biophysical, ecological, political and social consequences for human well‐being and ecosystem services. Understanding the process of land cover changes and transitions is essential for good ecosystem management policy that would lead to improved agricultural production, human well‐being and ecosystems health. This study aimed to assess land cover transitions in a typical semi‐arid degraded agro‐ecosystems environment within the Pangani river basin in northern Tanzania. Three Landsat images spanning over 30 years were used to detect random and systematic patterns of land cover transition in a landscape dominated by crop and livestock farming. Results revealed that current land cover transition is driven by a systematic process of change dominated by the following: (i) transition from degraded land to sparse bushland (10·8%); (ii) conversion from sparse bushland to dense bushland in lowland areas (6·0%); (iii) conversion from bushland to forest (4·8%); and (iv) conversion from dense bushland to cropland in the highlands (4·5%). Agricultural lands under water harvesting technology adoption show a high degree of persistence (60–80%) between time slices. This suggests that there is a trend in land‐use change towards vegetation improvement in the catchment with a continuous increase in the adoption of water harvesting technologies for crop and livestock farming. This can be interpreted as a sign of agricultural intensification and vegetation regrowth in the catchment. Copyright © 2015 John Wiley & Sons, Ltd.     

Age determination of large live trees with inner cavities: radiocarbon dating of Platland tree,a giant African baobab

• Introduction   

For large trees without a continuous sequence of growth rings in their trunk, such as the African baobab (Adansonia digitata L.), the only accurate method for age determination is radiocarbon dating. As of today, this method was limited to dating samples collected from the remains of dead specimens.     

Production of Pearl Millet Irrigated with Different Levels of Brackish Water and Organic Matter

ABSTRACT

The objective is to determine the growth, yield and chemical characteristics of pearl millet irrigated with different levels of brackish water and organic matter in two cultivation cycles. The experimental design was randomized blocks in a 4 × 4 factorial arrangement, composed of 4 levels of brackish irrigation (25, 50, 75 and 100% evapotranspiration), 4 levels of organic matter (0, 15, 30 and 45 t ha^?1) with 3 repetitions. Along two cultivation cycles, pearl millet crop was analyzed for: plant growth variables, fresh and dry mass production, water-use efficiency, and chemical composition. There was significant interaction between applied water levels and organic matter for lignin in the 2nd cut (P < .05). The number of leaves, number of dead leaves and fresh mass production were influenced by the isolated effect of brackish water levels in cut 1 (P < .05). In turn, fresh mass production, dry mass production, plant height, leaves length, panicle length, fresh mass production, crude protein and ether extract were influenced by the isolated effect of saline water levels in the 2nd cut (P < .05). Growth, biomass production and chemical composition variables in second cut are positively influenced by different brackish irrigation levels under low rainfall conditions.     

Monitoring of blood vessels and tissues by a population of monocytes with patrolling behavior

The cellular immune response to tissue damage and infection requires the recruitment of blood leukocytes. This process is mediated through a classical multistep mechanism, which involves transient rolling on the endothelium and recognition of inflammation followed by extravasation. We have shown, by direct examination of blood monocyte functions in vivo, that a subset of monocytes patrols healthy tissues through long-range crawling on the resting endothelium. This patrolling behavior depended on the integrin LFA-1 and the chemokine receptor CX(3)CR1 and was required for rapid tissue invasion at the site of an infection by this "resident" monocyte population, which initiated an early immune response and differentiated into macrophages.     

The illusion of control: industrialized agriculture,nature, and food safety

I explore the role of nature in the agrifood system and how attempts to fit food production into a large-scale manufacturing model has lead to widespread outbreaks of food borne illness. I illustrate how industrial processing of leafy greens is related to the outbreak of E. coli 0157:H7 associated with spinach in the fall of 2006. I also use this example to show how industry attempts to create the illusion of control while failing to address weaknesses in current processing systems. The leafy greens industry has focused efforts on sterilizing the growing environment and adopting new technologies, while neglecting to change the concentrated structure of processing systems. Repeated breakdowns in these systems illustrate a widening fault line between attempted and failed control of nature in industrial food production.

The site of attachment in human rhinovirus 14 for antiviral agents that inhibit uncoating

WIN 51711 and WIN 52084 are structurally related, antiviral compounds that inhibit the replication of rhino (common cold) viruses and related picornaviruses. They prevent the pH-mediated uncoating of the viral RNA. The compounds consist of a 3-methylisoxazole group that inserts itself into the hydrophobic interior of the VP1 beta-barrel, a connecting seven-membered aliphatic chain, and a 4-oxazolinylphenoxy group (OP) that covers the entrance to an ion channel in the floor of the "canyon." Viral disassembly may be inhibited by preventing the collapse of the VP1 hydrophobic pocket or by blocking the flow of ions into the virus interior.     

Measurement of the Motion of Fertilizer Particles Leaving a Centrifugal Spreader Using a Fast Imaging System

Although mechanically simple, centrifugal spreaders used for mineral fertilization involve complex physics that cannot be fully characterized at the present time. We are developing sensors to evaluate the spatial distribution of the fertilizer on the ground based on the measurement of initial flight conditions of fertilizer granules after their ejection by the spreading disk. The techniques developed are based on the analysis of images of the area around the disk showing the granule ejection. A high resolution – low cost imaging system for the analysis of high speed particle projection developed for this specific purpose is presented in this paper. The system, based on a camera and a sequence of flashes, is used to characterize the centrifugal spreading of fertilizer particles ejected at speeds of approximately 30ms^–1. It automatically computes the direction of ejection and velocity of each granule observed in the image. Multi-exposure images collected with the camera installed perpendicular to the output flow of granules are analyzed to estimate the trajectories of the fertilizer granules, using different motion estimation methods.     

Cuba's national food program and its prospects for food security

Cuba's National Food Program aims to assure its population a minimum degree of food security during the current period of transition from dependency upon the ex-Socialist trading bloc. A number of important elements of the Food Program, however, were conceived before the demise of COMECON in an effort to deepen food import substitution. This paper reviews the degree of Cuba's food import dependence before the breakup of the Socialist bloc, the initial targets of the National Food Program, and how these have been modified due to the severe reduction in Cuba's normal level of imports of petroleum and other agricultural inputs. It is argued that Cuba's reliance upon scientific advances combined with a return to traditional, ecologically-benign agricultural practices and large-scale labor mobilizations have allowed it to overcome a drastic shortfall in production as a result of the reduced level of imports of modern agricultural inputs. At the same time, it has been exceedingly difficult for the country to maintain production levels at the trend of the late 1980s or to continue to aspire to meet the ambitious targets of the initial Food Program plan.Carmen Diana Deere is Professor of Economics and Director of Latin American Studies at the University of Massachusetts, Amherst. The author has been carrying out field research in Cuba since the mid 1980s, in recent years, in a collaborative study with colleagues at the University of Havana. She is grateful to Carollee Bengelsdorf, Ernel Gonzales, Cristobal Kay, Mieke Meurs, Lupo Nuñez, Niurka Pérez, Brian Pollitt, and José Luís Rodríguez for their comments on earlier drafts. This article is an updated and condensed version of Institute of Social Studies Working Paper Series #124 (The Hague, 1992).     

A cost of long-term memory in Drosophila

Two distinct forms of consolidated associative memory are known in Drosophila: long-term memory and so-called anesthesia-resistant memory. Long-term memory is more stable, but unlike anesthesia-resistant memory, its formation requires protein synthesis. We show that flies induced to form long-term memory become more susceptible to extreme stress (such as desiccation). In contrast, induction of anesthesia-resistant memory had no detectable effect on desiccation resistance. This finding may help to explain why evolution has maintained anesthesia-resistant memory as another form of consolidated memory, distinct from long-term memory.