欧拉羊种公羊适应性观察与杂交改良乔科羊繁殖力分析

欧拉羊引入甘南州大水种畜场和碌曲县尕海乡、郎木寺镇等地改良乔科羊的肉用性能,引入的欧拉羊种公羊行为表现正常,抗寒、抗病能力较强,发病率为7％以下,死亡率在3％以下。繁殖性能和生长发育良好,主要生理生化指标都在正常范围之内。欧拉羊杂交改良乔科羊的繁殖力分析结果表明,用欧拉羊杂交改良乔科羊试验组的母羊受胎率、繁殖率、羔羊成活率及繁殖成活率均比当地乔科羊本交对照组分别提高1．45％、1．07％、4．06％和4．71％。     

The effects of canthaxanthin on porcine oocyte maturation and embryo development in vitro after parthenogenetic activation and somatic cell nuclear transfer

The objective of this study was to examine the effects of canthaxanthin (Cx) treatment during in vitro maturation (IVM) of porcine oocytes on embryonic development after parthenogenetic activation (PA) and somatic cell nuclear transfer (SCNT), on intracellular glutathione (GSH) and reactive oxygen species (ROS) levels in mature oocytes, and on gene expression in both PA‐ and SCNT‐derived blastocysts. To determine the optimal effective concentration of Cx, porcine oocytes were cultured in IVM medium supplemented with various concentrations (0, 20, 40 and 80 μM) of Cx for 22 hr. Compared to other groups, supplementation with 40 μM Cx significantly improved blastocyst formation rates after PA (p < .05), but no significant differences were observed among groups in total blastocyst cell numbers. Subsequently, oocytes were cultured in IVM medium supplemented with or without 40 μM Cx. Oocytes treated with 40 μM Cx showed significantly increased cleavage and blastocyst formation rates after SCNT compared to the control group (p < .05). Moreover, significantly increased intracellular GSH and reduced ROS levels were observed in the Cx‐treated group (p < .05). In addition, both PA‐ and SCNT‐derived blastocysts from the 40 μM Cx‐treated group showed significantly increased mRNA expression of Bcl2 and Oct4 and decreased Caspase3 expression level (p < .05), when compared with the control group. PA‐derived blastocysts from the 40 μM Cx‐treated group also exhibited significantly decreased expression of Bax (p < .05). Our results demonstrated that treatment with 40 μM Cx during IVM improves the developmental competence of PA and SCNT embryos. Improvement of embryo development by Cx is most likely due to increased intracellular GSH synthesis, which reduces ROS levels in oocytes, and it may also positively regulate apoptosis‐ and development‐related genes.     

Prevalence and risk factors for medical events following exercise at Australian Greyhound race meetings

Aim

A prospective, observational study to determine the prevalence of post‐exercise conditions at Australian Greyhound race meetings and to assess association with race performance and other environmental, race‐ and dog‐related factors was undertaken.

Methods

A total of 4020 starters were observed (2813 Greyhounds, 1009 trainers, 536 races, 52 race meets, 48 race dates and 11 race tracks) following a race. The presence of diaphragmatic flutter (DF), ataxia, seizure, collapse or sudden death was recorded. Risk factors were screened by univariable logistic regression prior to multivariable backward stepwise model building.

Results

In this study, 962 starters (n = 768 dogs) had DF (23.9%), 16 starters were ataxic (0.4%) and there were no observed cases of collapse, seizure or sudden death. Race track location, increasing race distance, race grade based on increasing 1st place prize value, lower (earlier) race number at the meeting, age, a previous observation of DF at the last start, females, colour (white) and better finishing position were all associated with an increased risk of a Greyhound being observed with DF. However, when logistic regression assessing the random effect of dog was performed, the presence of previous DF was not significant. In this cohort, DF was common following strenuous exercise in Greyhounds and on its own does not appear to result in reduced performance or distress to the animal.

Conclusion

The incidence of ataxia was low and collapse, seizure and sudden death were not observed. However, even though uncommon, ataxia has welfare concerns for racing Greyhounds that warrants further investigation.     

农牧民家庭档案管理系统的设计与实现

本文具体介绍了一个农牧民家庭档案管理软件系统,该系统采用数据分散收集、查询与统一集成处理相结合的两级数据库设计结构,既能适应目前基层乡镇独立建立农牧户家庭档案的发展现状,也为进一步向网络化管理平滑过渡建立了统一规范的数据基础.系统实现中针对家庭档案的特点解决了录入数据格式、录入项目灵活设置等问题,满足了家庭档案格式、主题随时间不断扩展、变化的需求.还实现了统计结果查询、多维度统计分析等数据分析的特色功能来为管理决策分析服务.这些功能为建立统一规范、可扩展的实用农牧民家庭档案提供了保障.使该系统成为具体落实农牧户家庭档案工作的一种有效实践手段.     

不同过瘤胃淀粉日粮对泌乳奶牛消化代谢的影响

以3头体况良好,安装有永久性瘤胃瘘管的泌乳前期奶牛为试验动物,在日粮精粗比为55∶45的前提条件下,采用3×3完全拉丁方试验设计,研究了3种不同过瘤胃淀粉日粮对泌乳奶牛消化代谢的影响。研究结果表明:3组不同日粮对营养物质的采食、排泄以及消化利用有着一定的规律性。3组不同日粮之间的采食量和消化率无明显差异(P>0.05);低过瘤胃淀粉日粮组的粪NDF排出量明显高于其他2组(P<0.05),其他2组之间差异不显著(P>0.05),粪中其他营养物质的排出量3组之间没有差异(P>0.05)。与对照组相比,其他2组均使尿氮排出量降低,其中高过瘤胃淀粉日粮组达到了显著水平(P<0.05),并且高过瘤胃淀粉日粮的氮沉积量和可消化氮转化为沉积氮的效率也明显高于其他2组(P<0.05),其他2组之间差异不显著。     

饲料中添加小苏打对甘南地区荷斯坦牛产奶量的影响

[目的]为研究饲料中添加小苏打对甘南地区荷斯坦牛产奶量的影响。[方法]选用27头健康、年龄、泌乳期一致的荷斯坦牛27头,随机分为3组,每组9头,设为对照组、试验A组、试验B组。分别饲喂8 kg青干草1、kg马铃薯;配合精料（见表2）;配合精料（见表2）＋150 g小苏打。[结果]试验A、B组比对照组日均多产奶3.75 kg和4.3 kg,分别提高了28%和32.1%（P〈0.05）,差异显著,试验B组较A组提高了0.55 kg（P〉0.05）,差异不显著。试验A、B组比对照组日均毛收入提高了7.53和9.65元,试验B组较A组日均毛收入提高了2.12元。[结论]在荷斯坦牛饲料中添加150 g小苏打能有效提高产奶量。     

乔科型藏羊遗传资源保护及制种供种体系建设思路

作者通过调查乔科型藏羊遗传资源现状,发现乔科型藏羊纯种数量当前仍有不断下降的风险,为了避免乔科型藏羊种群数量不断减少,作者分析了乔科型藏羊种质资源的优良特性,总结了开展乔科型藏羊遗传资源保护的意义,提出了乔科型藏羊遗传资源保护及制种供种体系建设的思路,并提出了建议。