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311.
Marília Teresa de Oliveira Joo Pedro Scussel Feranti Gabriela Pesamosca Coradini Rafael Oliveira Chaves Luis Felipe Dutra Corrêa Marcella Teixeira Linhares Roberto Thiesen Marco Augusto Machado Silva Maurício Veloso Brun 《Journal of veterinary science (Suw?n-si, Korea)》2021,22(3)
BackgroundIntraoperative fluids are still poorly studied in veterinary medicine. In humans the dosage is associated with significant differences in postoperative outcomes.ObjectivesThe aim of this study is to verify the influence of three different fluid therapy rates in dogs undergoing video-assisted ovariohysterectomy.MethodsTwenty-four female dogs were distributed into three groups: G5, G10, and G20. Each group was given 5, 10, and 20 mL·kg−1·h−1 of Lactate Ringer, respectively. This study evaluated the following parameters: central venous pressure, arterial blood pressure, heart rate, respiratory rate, temperature, acid-base balance, and serum lactate levels. Additionally, this study evaluated the following urinary variables: urea, creatinine, protein to creatinine ratio, urine output, and urine specific gravity. The dogs were evaluated up to 26 h after the procedure.ResultsAll animals presented respiratory acidosis during the intraoperative period. The G5 group evidenced intraoperative oliguria (0.80 ± 0.38 mL·kg−1·h−1), differing from the G20 group (2.17 ± 0.52 mL·kg−1·h−1) (p = 0.001). Serum lactate was different between groups during extubation (p = 0.036), with higher values being recorded in the G5 group (2.19 ± 1.65 mmol/L). Animals from the G20 group presented more severe hypothermia at the end of the procedure (35.93 ± 0.61°C) (p = 0.032). Only the members of the G20 group presented mean potassium values below the reference for the species. Anion gap values were lower in the G20 group when compared to the G5 and G10 groups (p = 0.017).ConclusionsThe use of lactated Ringer''s solution at the rate of 10 mL·kg−1·h−1 seems to be beneficial in the elective laparoscopic procedures over the 5 or 20 mL·kg−1·h−1 rates of infusion. 相似文献
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313.
Luciana Lasry Benchimol Tatiana de Campos Sérgio Augusto Morais Carbonell Carlos Augusto Colombo Alisson Fernando Chioratto Eduardo Fernandes Formighieri Lígia Regina Lima Gouvêa Anete Pereira de Souza 《Genetic Resources and Crop Evolution》2007,54(8):1861-1762
A common bean genomic library was constructed using the ‘IAC-UNA’ variety enriched for (CT) and (GT) for microsatellite motifs.
From 1,209 sequenced clones, 714 showed microsatellites distributed over 471 simple and 243 compound motifs. GA/CT and GT/CA
were the most frequent motifs found among these sequences. A total of 123 microsatellites has been characterized. Out of these,
87 were polymorphic (73.7%), 33 monomorphic (26.8%), and 3 (2.4%) did not amplify at all. In a sample of 20 common bean materials
selected from the Agronomic Institute Germplasm Bank, the number of alleles per locus varied 2–9, with an average of 2.82.
The polymorphic information content (PIC) of each marker varied from 0.05 to 0.83, with a 0.45 average value. Cluster and
principal coordinate analysis of the microsatellite data were consistent with the original assignment of the germplasm accessions
into the Andean and Mesoamerican gene pools of common bean. Low polymorphism levels detected could be associated with the
domestication process. These microsatellites could be a valuable resource for the bean community because of their use as new
markers for genetic studies.
Electronic supplementary material The online version of this article (doi: ) contains supplementary material, which is available to authorized users.
An erratum to this article can be found at 相似文献
314.
Miranda-Rottmann S Aspillaga AA Pérez DD Vasquez L Martinez AL Leighton F 《Journal of agricultural and food chemistry》2002,50(26):7542-7547
Oxidative modification of low-density lipoprotein (LDL) particles is a key event in the development of atherosclerosis. Oxidized LDL induces oxidative stress and modifies gene expression in endothelial cells. Berries constitute a rich dietary source of phenolic antioxidants. We found that the endemic Chilean berry Aristotelia chilensis (ach) has higher phenol content and scores better for total radical-trapping potential and total antioxidant reactivity in in vitro antioxidant capacity tests, when compared to different commercial berries. The juice of ach is also effective in inhibiting copper-induced LDL oxidation. In human endothelial cell cultures, the addition of ach juice significantly protects from hydrogen peroxide-induced intracellular oxidative stress and is dose-dependent. The aqueous, anthocyanin-rich fraction of ach juice accounts for most of ach's antioxidant properties. These results show that ach is a rich source of phenolics with high antioxidant capacity and suggest that it may have antiatherogenic properties. 相似文献
315.
316.
A rapid sample screening method for authenticity control of whiskey using capillary electrophoresis with online preconcentration 总被引:1,自引:0,他引:1
Heller M Vitali L Oliveira MA Costa AC Micke GA 《Journal of agricultural and food chemistry》2011,59(13):6882-6888
The present study aimed to develop a methodology using capillary electrophoresis for the determination of sinapaldehyde, syringaldehyde, coniferaldehyde, and vanillin in whiskey samples. The main objective was to obtain a screening method to differentiate authentic samples from seized samples suspected of being false using the phenolic aldehydes as chemical markers. The optimized background electrolyte was composed of 20 mmol L(-1) sodium tetraborate with 10% MeOH at pH 9.3. The study examined two kinds of sample stacking, using a long-end injection mode: normal sample stacking (NSM) and sample stacking with matrix removal (SWMR). In SWMR, the optimized injection time of the samples was 42 s (SWMR42); at this time, no matrix effects were observed. Values of r were >0.99 for the both methods. The LOD and LOQ were better than 100 and 330 mg mL(-1) for NSM and better than 22 and 73 mg L(-1) for SWMR. The CE-UV reliability in the aldehyde analysis in the real sample was compared statistically with LC-MS/MS methodology, and no significant differences were found, with a 95% confidence interval between the methodologies. 相似文献
317.
Edison Barbieri Leandro Augusto Alves Ferreira 《Pesticide biochemistry and physiology》2011,99(3):209-214
Nile Tilapia (Oreochromis niloticus) juveniles were exposed to different concentrations of Folidol 600® in static toxicity tests. The 24, 48, 72 and 96 h LC50 values of Folidol 600® to O. niloticus were 17.82, 8.91, 4.00 and 2.70 mg L−1, respectively. The values of hematological parameters increased, and inhibition of cholinesterases activity (AChE, BChE and PChE) in plasma of fish exposed to the higher concentrations of pesticide reached 94%. Furthermore, the exposure of Tilapia to Folidol 600® caused an increase of 4%, 20% and 38.4% in oxygen consumption at 0.1, 0.5 and 1.0 mg L−1, respectively. However, exposure to 2.5, 5.0 and 10 mg L−1 caused a decrease of 33.6%, 35.2% and 42.4% in oxygen consumption relative to the control. The ammonium excretion of fish exposed to 0.0, 0.1, 0.5, 1.0, 2.5, 5.0 and 10.0 mg Folidol 600®/L was 0.12, 0.18, 0.30, 0.33, 0.37, 0.36 and 0.33 μg/g/min, i.e., 50%, 150%, 175%, 208%, 200% and 175% increase, respectively, relative to the control. 相似文献
318.
319.
Farias Nadja Naiara Pereira Freitas Ednardo Rodrigues do Nascimento Germano Augusto Jerônimo Xavier Regina Patrícia Souza de Melo Braz Nádia Dantas Francisco Diego Teixeira Figueiredo Carlos Weiber Silva Gomes Valter Luis Maia Watanabe Pedro Henrique 《Tropical animal health and production》2019,51(1):179-185
Tropical Animal Health and Production - The aim of the current study was to evaluate the addition of fresh and stored copra meal to the diet of meat quails. Two hundred eighty-seven-day-old male... 相似文献
320.
Ederli NB de Oliveira FC Lopes CW Damatta RA Santos Cde P de Azevedo Rodrigues Mde L 《Veterinary parasitology》2008,155(3-4):323-327
The differentiation of the species of the Libyostrongylus genus is only possible with the obtainment of the adult parasites in the ostriches proventriculus and gizzard. The present work confirms that it is possible to differentiate the infective larvae of L. douglassii and L. dentatus allowing the differential diagnosis of these species by fecal culture. To show this, adult females from both species were collected from ten proventriculus from adult ostriches and separated by species. Both groups were macerated individually added to sterilized feces for standard fecal cultures. The infective larvae were recovered, identified, quantified and measured. All proventriculus analyzed were parasitized by Libyostrongylus spp. and a clear heterogeneous location for each species was observed. The infective larvae from the fecal cultures of macerated L. douglassii presented a mean total length of 874.3+/-33.80 microm, and a short sheath tail (29.5+/-4.11 microm) with acute termination. The infective larvae from the macerated L. dentatus presented mean total length of 856.0+/-43.63 microm, long sheath tail (61.2+/-9.52 microm) with filamentous termination. The mean measures of the tails of both species had a significant difference. The differentiation of the infective larvae of L. douglassii and L. dentatus by fecal cultures will facilitate the diagnosis of both species for further understanding the Libyostrongylus biology. 相似文献