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71.
Y Makovsky SL Klemperer L Ratschbacher LD Brown M Li W Zhao F Meng 《Science (New York, N.Y.)》1996,274(5293):1690-1691
Three-component wide-angle seismic data acquired in southern Tibet during Project INDEPTH show strong P-to-S converted reflections from reflectors that are aligned at a depth of approximately 15 kilometers beneath the northern Yadong-Gulu rift. These converted reflections are locally higher in amplitude than the corresponding P-wave reflections. Modeling of reflection mode conversion as a function of incidence angle indicates that this condition obtains for a reflector that is a solid over fluid interface; it is not typical of a solid-solid interface. The likely candidates for a fluid trapped within the crystalline crust of southern Tibet are granitic magma and water (brine). 相似文献
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74.
Abnormal cytokinesis in cells deficient in the breast cancer susceptibility protein BRCA2 总被引:1,自引:0,他引:1
Germ-line mutations inactivating BRCA2 predispose to cancer. BRCA2-deficient cells exhibit alterations in chromosome number (aneuploidy), as well as structurally aberrant chromosomes. Here, we show that BRCA2 deficiency impairs the completion of cell division by cytokinesis. BRCA2 inactivation in murine embryo fibroblasts (MEFs) and HeLa cells by targeted gene disruption or RNA interference delays and prevents cell cleavage. Impeded cell separation is accompanied by abnormalities in myosin II organization during the late stages in cytokinesis. BRCA2 may have a role in regulating these events, as it localizes to the cytokinetic midbody. Our findings thus link cytokinetic abnormalities to a hereditary cancer syndrome characterized by chromosomal instability and may help to explain why BRCA2-deficient tumors are frequently aneuploid. 相似文献
75.
Previous studies of anaerobic biocorrosion have suggested that microbial sulfur and phosphorus products as well as cathodic hydrogen consumption may accelerate anaerobic metal oxidation. Methanogenic bacteria, which normally use molecular hydrogen (H(2)) and carbon dioxide (CO(2)) to produce methane (CH(4)) and which are major inhabitants of most anaerobic ecosystems, use either pure elemental iron (Fe(0)) or iron in mild steel as a source of electrons in the reduction of CO(2) to CH(4). These bacteria use Fe(0) oxidation for energy generation and growth. The mechanism of Fe(0) oxidation is cathodic depolarization, in which electrons from Fe(0) and H(+) from water produce H(2), which is then released for use by the methanogens; thermodynamic calculations show that significant Fe(0) oxidation will not occur in the absence of H(2) consumption by the methanogens. The data suggest that methanogens can be significant contributors to the corrosion of iron-containing materials in anaerobic environments. 相似文献
76.
Daniels F 《Science (New York, N.Y.)》1903,18(450):215-217
77.
Atomic structure of ferredoxin-NADP+ reductase: prototype for a structurally novel flavoenzyme family 总被引:27,自引:0,他引:27
The three-dimensional structure of spinach ferredoxin-NADP+ reductase (NADP+, nicotinamide adenine dinucleotide phosphate) has been determined by x-ray diffraction at 2.6 angstroms (A) resolution and initially refined to an R factor of 0.226 at 2.2 A resolution. The model includes the flavin-adenine dinucleotide (FAD) prosthetic group and the protein chain from residue 19 through the carboxyl terminus at residue 314 and is composed of two domains. The FAD binding domain (residues 19 to 161) has an antiparallel beta barrel core and a single alpha helix for binding the pyrophosphate of FAD. The NADP binding domain (residues 162 to 314) has a central five-strand parallel beta sheet and six surrounding helices. Binding of the competitive inhibitor 2'-phospho-AMP (AMP, adenosine monophosphate) places the NADP binding site at the carboxyl-terminal edge of the sheet in a manner similar to the nucleotide binding of the dehydrogenase family. The structures reveal the key residues that function in cofactor binding and the catalytic center. With these key residues as a guide, conclusive evidence is presented that the ferredoxin reductase structure is a prototype for the nicotinamide dinucleotide and FAD binding domains of the enzymes NADPH-cytochrome P450 reductase, NADPH-sulfite reductase, NADH-cytochrome b5 reductase, and NADH-nitrate reductase. Thus this structure provides a structural framework for the NADH- or NADPH-dependent flavoenzyme parts of five distinct enzymes involved in photosynthesis, in the assimilation of inorganic nitrogen and sulfur, in fatty-acid oxidation, in the reduction of methemoglobin, and in the metabolism of many pesticides, drugs, and carcinogens. 相似文献
78.
Daniels RS 《Science (New York, N.Y.)》1979,204(4389):124
79.
Thomas L. Daniels 《Agriculture and Human Values》1991,8(3):3-9
The goals and values of economic development strategies vary according to the individual communities that employ them. While economic development strategies are aimed at increasing jobs, income, and community wealth, the issue of who gains and who loses from economic change is often overlooked. The industrial development strategies of the 1960s and 1970s are giving way to local initiatives based on services. Although local efforts may mean greater local control, the globalization of the economy has exposed formerly remote areas to international competition. The challenge to communities will be to achieve a moderate, steady, and manageable pace of good growth. Each community will ultimately need to develop a strategy for economic growth that matches community desires with community resources.Thomas L. Daniels is Director of the Agricultural Preserve Board of Lancaster County, Pennsylvania. He is co-author ofRural Planning and Development in the United States, and has written several articles on rural ecconomic development. 相似文献
80.