BA-ELISA快速检测鱼源沙门氏菌的研究

本研究建立了快速检测鱼源沙门氏菌的BA-ELISA工作程序。该程序灵敏度为105个/mL,可经受108个/mL杂菌的干扰,对含有10个以上沙门氏菌的接种标本经21～23h两步增菌后即可检出,可在25～27h内报告结果,比常规分离培养方法缩短3～5d。从6个品种的498尾淡水鱼和7个品种的160尾海水鱼,共采集标本778份,以常规分离培养法检出29份阳性标本,分离率为3.7%。对包括该29份分离阳性标本在内的184份鱼源标本实施BA-ELISA的结果表明,两法的符合率为96.73%,BA-ELISA法的敏感性为100%(29/29),特异性为96.1%(149/155)。独立性检验表明,两法检测结果间有极显著意义的一致性(P<0.01)。血清学分型表明,分离的菌株全部为鼠伤寒沙门氏菌(1,4,5,12:i:1,2)。     

畜禽场空气污染对人畜健康的影响

<正>随着畜禽规模化养殖生产的发展及公众对环境要求的提高,畜禽场的空气污染问题已引起国内外有关机构和研究者的重视。欧美国家规模化养殖发展较我国早,在20世纪80年代他们就已经展开了畜禽场污染气体对动物与人体健康影响的研究[1]。近几年,畜禽场空气污染问题更是倍受重视,其研究已经涉及畜禽场空气污染物成分分析、污染物去除方法研究、     

Green tea polyphenols supplementation alters immunometabolism and oxidative stress in dairy cows with hyperketonemia

Peripartal cows often experience negative energy balance, and are therefore prone to suffering from metabolic diseases such as hyperketonemia, which causes financial losses in dairy farms. This study aimed to investigate the effect of green tea polyphenol (GTP) supplementation during the periparturient period on production performance, oxidative stress and immunometabolism in dairy cows with hyperketonemia. One hundred Holstein cows were assigned to GTP (0.2 g/kg DM; n = 50) or control (without GTP; n = 50) group based on body weight, previous milk yield, and parity on d 15 before expected parturition. Subsequently, 10 cows with hyperketonemia were selected from each group, according to blood β-hydroxybutyric acid (BHBA) concentration between 1.2 and 2.9 mmol/L from 2 to 3 d postpartum. All cows were fed a close-up diet and a lactation diet with or without GTP supply from 15 d prepartum until 30 d postpartum. Milk and blood samples were obtained from 20 cows selected with hyperketonemia on 10, 20, and 30 d postpartum. Compared with control cows, greater milk yield and lower somatic cell count were observed in GTP cows. The GTP group had lower concentrations of BHBA, free fatty acids, cholesterol, triglyceride, reactive oxygen species, malondialdehyde, and hydrogen peroxide, greater concentrations of glucose, lower activities of aspartate aminotransferase, alanine aminotransferase, and glutamyl transpeptidase, alongside greater activities of superoxide dismutase, glutathione peroxidase, and total antioxidant capacity. Additionally, GTP supplementation up-regulated concentrations of interleukin-6 and interleukin-10, but down-regulated concentrations of tumor necrosis factor-α, interleukin-1β, interleukin-2, interleukin-8, and interferon-γ in plasma. Greater concentrations of plasma immunoglobulin G were also detected in the GTP group. Overall, the data suggested that GTP supplementation from 15 d prepartum to 30 d postpartum improved the milk yield and health status in cows with hyperketonemia during early lactation.     

果蔬产品变色原因分析及其控制

分析了果蔬产品在加工贮存中变色的原因,综述了目前生产中常用的行之有效的护色措施,包括生物、化学和物理等方面的处理方法。     

Study on Modeling for Pretreatment and Cyclic Control Stream

The characteristics of pretreatment and cyclic control stream are analysed:the form of cyclic embedded hardware circuit,the idea for designing the pretreatment program.The chart model of the system is established preliminarily.     

PROBABILISTIC EVALUATION FOR STEEL FRAME COLUMNS OF TALL BUILDINGS

Based on properties of parameters and test data of dom-estic steel,this paper analyses the reliability and partial coefficient forresistance of steel frame columns of tall builings.The result may serveas a scientific basis.It is not only for domestic steel instead of importedsteel in building tall steel structures,but also for compiling concerendstandards.     

Sociological Factors about the Urbanization of Traditional Community --Discussion on Ciqikou Community

The modernization has been the dream that Chinese nation has wished eagerly to realize all the time. In some sense, the modernization of China means urbanization. The reform and opening - up over the past 20 years , built and made the enormous achievement in urbanization of China, have changed the appearance of the city of China greatly. But to numerous traditional historical zones, what kind of urbanization way should on earth be taken, it has been the question long - term in dispute of the planning circles all the time. In this paper, Ciqikou community of Chongqing as an example, doing some discussions from sociology on this question, the author hopes, that for such difficult problem, by this settlement a piece of breach can be found as soon as possible.     

Application of Water Quality Standand Leuel Method in Eualuazing Status Quo of Heauy-Metals Pollution in Riuers

The status quo of heauy-metals pollution in water phase of course of Long-Tan hydropower statian was eualuated by water Quality Standand Level Method. With this special method,mone objectiue and definite results were obtained.     

王家厂水库洪水实时预报方法及应用研究

考虑到流域降雨时空变化和流域地形、河道特征对流域产汇流的影响，应用新安江三水源产流模型、流域分散入流非线性汇流模型以及流域洪水实时预报校正模型，对王家厂流域洪水进行模拟，取得了较为满意的计算结果。     

凡纳对虾小触角表面结构的显微观察

应用光学显微镜和扫描电子显微镜观察了凡纳对虾小触角的表面结构。结果发现：小触角上广泛着生着700根以上的羽状刚毛；外鞭片状隆起腹面平均约具280根无顶孔但有细分支的化感刚毛，这类有分支的化感刚毛在其它十足目种类上未见有报道，而且其刚毛数也较其它种类少；内侧附肢前端与柄节上直立的羽状刚毛毛簇顶相接触，推测该结构可能对相邻的复眼具某种作用。