Fungal endophyte Phomopsis liquidambari B3 enriches the diversity of nodular culturable endophytic bacteria associated with continuous cropping of peanut

Previously, the application of the fungal endophyte Phomopsis liquidambari B3 exhibited notable effect in alleviating soil obstacles caused by continuous cropping of peanut. It was supposed that P. liquidambari B3 could enhance the efficiency of nodulation and nitrogen fixation by enriching the diversity of nodular endophytic bacteria under continous monocropping system. To verify this hypothesis, a pot experiment was conducted under natural conditions. Four treatments were set as follow: discontinuous cropping soil (NS), continuous cropping soil (CK), continuous cropping soil inoculate with actived P. liquidambari B3 (CSB3), and continuous cropping soil inoculate with sterilized P. liquidambari B3 (CSIB3). As a result, a total of 120 isolates were obtained. Our study clearly declared that the addition of fungal endophyte effectively enriched the genetic diversity and the community composition in CSB3. In contrast, a low genetic diversity level and simplex community structure were exhibited in CK and CSIB3. Meanwhile, the positive percentage rate and corresponding community composition of the plant growth-promoting isolates in NS and CSB3 were larger and more abundant than the other. This is the first time to describe the effects of fungal endophytes on the diversity of the nodular culturable endophytic bacteria associated with continuous cropping of peanut.     

电子鼻和电子舌单独与联合检测掺大豆蛋白或淀粉的鸡肉糜

为实现掺杂掺假鸡肉的快速、客观评价,该研究利用电子鼻和电子舌联合检测技术对掺杂鸡肉糜进行快速检测,通过对采集的数据进行主成分分析和偏最小二乘法分析,所得结果表明,采用主成分分析,电子鼻和电子舌联合检测掺大豆蛋白鸡肉糜和掺淀粉鸡肉糜的主成分总贡献率分别为99.8%和99.1%;采用偏最小二乘法分析,电子鼻和电子舌联合检测鸡肉糜中掺杂大豆蛋白含量的预测值与真实值之间的决定系数为0.992,均方根误差为2.8%;联合检测鸡肉糜中掺杂淀粉含量的预测值与真实值之间的决定系数为0.996,均方根误差为2.4%。表明电子鼻和电子舌联合检测对鸡肉糜的掺杂情况具有良好的区分和预测能力,并且是一种有效、高精度的肉类掺假检测方法。     

生物炭提高花生干物质与养分利用的优势研究

【目的】 以秸秆为原料生产生物炭可用于改良土壤和提高养分利用率,其与秸秆直接还田以及传统的制作堆肥后还田相比是否具有优势需要用试验来验证,本研究可为生物炭的高效利用提供理论依据。 【方法】 以传统猪厩肥和秸秆直接还田为对照,连续进行了8年的花生田间微区 (2 m2) 试验。在氮磷钾总投入量相等的条件下,共设4个处理,分别为秸秆还田 (CS)、猪厩肥 (PMC)、生物炭 (BIO) 和基于生物炭的炭基花生专用肥 (BF),每个处理重复3次,随机区组排列。试验于2016年在花生苗期、开花下针期、结荚期和饱果成熟期进行采样,测定植株茎叶、根和荚果的干物质和氮磷钾养分积累量,并计算对应的分配情况,探讨其对花生产量的影响。 【结果】 生物炭处理的花生产量显著高于其它处理,达到7231.7 kg/hm2;生物炭复合肥和猪厩肥处理则相对较低,分别是生物炭处理的82.4%和83.8%,秸秆处理产量最低,为5623.9 kg/hm2。猪厩肥处理的出仁率显著高于其它处理。生育前期各处理的干物质和养分主要在茎叶中积累,从结荚期开始逐渐向荚果中转移。与对照处理相比,复合肥处理的干物质和氮磷钾养分整株积累量在各时期均较高,尤其在结荚期以前保持了良好的荚果干物质和养分分配系数;生物炭处理则至饱果成熟期时呈现出明显优势,干物质积累量达到6295.0 kg/hm2,分别高出专用肥、秸秆和猪厩肥处理43.1%、36.1%和50.8%,茎叶分配比例高达34.5%,氮、磷、钾积累量持续增长至236.4 kg/hm2、 21.7 kg/hm2、77.8 kg/hm2,显著高于其它处理,但此时期荚果的氮、钾分配系数仅有0.83和0.52,低于对照处理(CS、PMC处理) 0.02～0.03和0.15～0.21。 【结论】 在氮磷钾养分投入量相等、不考虑有机碳投入量的前提下,施用生物炭、炭基复合肥和猪厩肥效果均显著好于秸秆直接还田;生物炭可显著提高花生整株的干物质量和氮磷钾积累量,特别是提高生育后期的干物质和养分分配量,促进产量的提高,对花生高产增效有良好的促进作用;炭基复合肥在花生进入结荚期后,对花生干物质及养分积累分配的促进作用减弱,效果与施用猪厩肥相当。因此,在本试验条件下,生物炭直接施用具有维持其养分长期稳定释放,提高花生产量和肥料养分利用率的作用。      

发酵床垫料翻耙结合网床养殖改善鸭舍空气质量与鸭生产性能

为解决规模化肉鸭生产中粪污影响鸭健康和污染环境的问题,研发了一种发酵床结合网床架养新技术及其配套的可移动式网床下发酵床垫料翻耙系统,并研究了该模式在控制舍内气载微生物数量和提高鸭生产性能中的优势。结果显示:1)可移动式发酵床垫料翻耙系统实现不同发酵床体共用一台翻耙机。2)与发酵床平养相比,鸭26日龄之前未翻耙垫料时,发酵床网养舍内需氧菌总数(1.531×105 CFU/m3)和大肠杆菌数量(1.298×104 CFU/m3)显著升高(P0.05);鸭27~34日龄时每4 d翻耙垫料,发酵床网养舍内需氧菌总数(2.304×105 CFU/m3)和金黄色葡萄球菌数量(1.353×105 CFU/m3)显著降低(P0.05);鸭35~39日龄时每天翻耙垫料,发酵床网养舍内需氧菌总数(1.255×105 CFU/m3)和"沙门+志贺"氏菌数量(14.13 CFU/m3)显著降低(P0.05)。3)在发酵床平养舍内,与每4 d翻耙1次垫料相比,每天翻耙1次垫料舍内需氧菌总数(2.688×105 CFU/m3)、大肠杆菌(2.038×104 CFU/m3)、金黄色葡萄球菌(8.90×104 CFU/m3)和"沙门+志贺"氏菌(47.11 CFU/m3)数量显著(P0.05)降低,而在发酵床网养舍内,每天翻耙1次垫料舍内需氧菌总数(1.255×105 CFU/m3)和"沙门+志贺"氏菌(14.13 CFU/m3)数量显著降低(P0.05)。4)与发酵床平养相比,发酵床网养增加鸭体质量(P0.05),降低死亡率和上市淘汰率(P0.05)。结果表明研发的肉鸭养殖新模式能更好地减少舍内空气中病原菌浓度,改善鸭舍内空气环境,提高鸭生产性能。该研究为发酵床结合网床架养新模式在规模企业中推广应用提供技术支持。     

pH-dependent distribution of soil ammonia oxidizers across a large geographical scale as revealed by high-throughput pyrosequencing

Purpose

Ammonia-oxidizing archaea (AOA) and bacteria (AOB) are ubiquitous and important for nitrogen transformations in terrestrial ecosystems. However, the distribution patterns of these microorganisms as affected by the terrestrial environments across a large geographical scale are not well understood. This study was designed to gain insights into the ecological characteristics of AOA and AOB in 65 soils, collected from a wide range of soil and ecosystem types.

Materials and methods

Barcoded pyrosequencing in combination with quantitative PCR was employed to characterize the relative abundance, diversity, and community composition of archaeal 16S rRNA gene, and AOA and AOB amoA genes in 65 soil samples.

Results and discussion

The operational taxonomic unit richness and Shannon diversity of Thaumarchaeota, AOA, and AOB were highly variable among different soils, but their variations were best explained by soil pH. Soil pH was strongly correlated with the overall community composition of ammonia oxidizers, as measured by the pairwise Bray–Curtis dissimilarity across all sites. These findings were further corroborated by the evident pH-dependent distribution patterns of four thaumarchaeal groups (I.1a-associated, I.1b, I.1c, and I.1c-associated) and four AOB clusters (2, 3a.1, 10, and 12). The ratios of AOA to AOB amoA gene copy numbers significantly decreased with increasing pH, suggesting a competitive advantage of AOA over AOB in acidic soils.

Conclusions

These results suggest that the distribution of ammonia oxidizers across large-scale biogeographical settings can be largely predicted along the soil pH gradient, thus providing important indications for the ecological characteristics of AOA and AOB in different soils.     

鱼稻共生条件下陶粒载体水稻浮床提高细菌活性及多样性

以轻质陶粒为主要原料开发出一种新型生物浮床载体,并通过种植水稻构建了陶粒载体水稻浮床对池塘水环境进行修复。研究了陶粒载体水稻浮床对精养池塘的水质净化作用,并采用Biolog-ECO技术分析了陶粒载体水稻浮床对池塘浮游细菌碳源代谢模式和多样性的影响。结果显示,试验组池塘水中总氮(TN)、总磷(TP)和亚硝态氮(NO2–-N)等水质指标在8月份显著低于对照组,高锰酸盐指数(IMn)在8月份和10月份显著低于对照组。试验组池塘浮游细菌群落代谢活性(AWCD)、Shannon指数和丰富度指数在8月份显著高于对照塘,Shannon均匀度指数在10月份显著高于对照塘。浮床种植水稻产量达到5 900 kg/hm~2。结果表明陶粒载体水稻浮床在具备一定生产性能基础上,降低了精养池塘水中氮磷营养水平,改变了精养池塘浮游细菌群落碳源利用模式,并提高了池塘浮游细菌群落代谢活性和功能多样性。结果可为应用陶粒载体水稻浮床调控池塘水环境提供理论指导。     

Effect of Assessment Scale on Spatial and Temporal Variations in CH4, CO2, and N2O Fluxes in a Forested Wetland

Emissions of methane (CH₄), carbon dioxide (CO₂), and nitrous oxide (N₂O) from a forested watershed (160 ha) in South Carolina, USA, were estimated with a spatially explicit watershed-scale modeling framework that utilizes the spatial variations in physical and biogeochemical characteristics across watersheds. The target watershed (WS80) consisting of wetland (23%) and upland (77%) was divided into 675 grid cells, and each of the cells had unique combination of vegetation, hydrology, soil properties, and topography. Driven by local climate, topography, soil, and vegetation conditions, MIKE SHE was used to generate daily flows as well as water table depth for each grid cell across the watershed. Forest-DNDC was then run for each cell to calculate its biogeochemistry including daily fluxes of the three greenhouse gases (GHGs). The simulated daily average CH₄, CO₂ and N₂O flux from the watershed were 17.9 mg C, 1.3 g C and 0.7 mg N m⁻², respectively, during the period from 2003–2007. The average contributions of the wetlands to the CH₄, CO₂ and N₂O emissions were about 95%, 20% and 18%, respectively. The spatial and temporal variation in the modeled CH₄, CO₂ and N₂O fluxes were large, and closely related to hydrological conditions. To understand the impact of spatial heterogeneity in physical and biogeochemical characteristics of the target watershed on GHG emissions, we used Forest-DNDC in a coarse mode (field scale), in which the entire watershed was set as a single simulated unit, where all hydrological, biogeochemical, and biophysical conditions were considered uniform. The results from the field-scale model differed from those modeled with the watershed-scale model which considered the spatial differences in physical and biogeochemical characteristics of the catchment. This contrast demonstrates that the spatially averaged topographic or biophysical conditions which are inherent with field-scale simulations could mask “hot spots” or small source areas with inherently high GHGs flux rates. The spatial resolution in conjunction with coupled hydrological and biogeochemical models could play a crucial role in reducing uncertainty of modeled GHG emissions from wetland-involved watersheds.     

草本植物根系网的固土机制模式与力学试验研究

针对生物软措施的力学固土护坡作用进行了力学机制方面的探讨,提出植物根系网固土力学机制模式的四个层次,以及对不同草本植物根系的固土性能进行了力学试验比较。结果表明不同植物根系具有不同的抗拉强度。香根草根系平均抗拉强度最大达85 m Pa,其次为假俭草,平均抗拉强度27.3 m Pa,白三叶根系平均抗拉强度为24.6 m Pa,莎草根系平均抗拉强度为24.5 m Pa,宜安草根系平均抗拉强度为19.7 m Pa,百喜草根系平均抗拉强度为19.23 m Pa,马尼拉草根系平均抗拉强度为17.5 m Pa,狗牙根根系平均抗拉强度为13.45 m Pa。不同的植物根系具有不同的抗拉强度、不同的固土性能与其遗传结构及其组织材料不同有关。充分了解和认识植物根系固土性能,科学合理运用生物软措施代替或部分代替工程措施在我国繁重的生态环境保护和恢复的基本建设工程项目中具有重要的应用价值和意义。     

循环式海绵基质培设施结构和性能的研究与应用

循环式海绵基质培是根据世界无土栽培技术发展趋势和中国具体国情进行试验设计、研制而成的新型无土栽培设备.其主要特点是1)基质用量少,成本低;2)供排液分开,避免根际病害传播;3)配套营养液生物过滤装置,提高系统安全稳定性;4)营养液供给自动控制.循环式海绵基质培由贮液池、农用海绵基质(黑白双色膜包被)、双层槽式集液系统、营养液滴灌系统及自控设备、营养液生物过滤系统等组成.循环式海绵基质培在中国东南沿海地区一年量达10000 kg以上,亩产值1.5万元以上,适合农业示范园区内的大型温室应用.     

WRKY转录因子及其在植物防御反应中的作用

WRKY蛋白是只存在于植物中较为复杂的转录因子家族。由WRKY蛋白N端高度保守的WRKYGQK氨基酸序列及其特殊的锌指结构而命名的WRKY结构域能专性与其顺式作用元件W-盒[(T)(T)TGAC(C／T)]结合。根据WRKY结构域的数量及锌指结构的特征将WRKY蛋白家族分为3类,其中拟南芥中第三类家族中几乎所有的WRKY成员都与应答生物胁迫有关。WRKY蛋白的表达特性为快速、瞬时、具组织特异性诱导表达。目前,WRKY蛋白被广泛认为参与植物生物与非生物胁迫应答反应、植物衰亡、种皮及毛状体发育及果实发育等一系列的生理活动,本文综述了WRKY转录因子的结构特征及其功能特性,重点讨论了它们在植物防卫反应中的调控作用。