磷壁酸在细菌黏附、定植及免疫调节过程中的作用

磷壁酸是革兰氏阳性菌细胞壁的主要成分,在细菌自我保护、离子平衡、细胞分裂及其与宿主细胞互作等过程中发挥着重要生理作用。本文综述了磷壁酸在革兰氏阳性菌黏附、定植及免疫调节过程中的作用,阐述了磷壁酸识别的细胞膜受体蛋白,旨在为深入解析细菌在肠黏膜细胞膜上的黏附定植机制提供新线索。     

大理市绿地系统树种规划研究

在对大理市绿地系统中园林绿化树种应用现状调查的基础上,分析了其树种规划存在的问题,结合市情,探讨了大理市树种规划的基本原则,提出了树种规划的主要方式.     

果树病毒核酸检测技术研究进展

病毒的核酸检测技术发展非常迅速,主要有PCR技术、分子杂交技术、dsRNA技术、NASBA技术和基因芯片技术。综述了这些技术的原理、特点及其在果树病毒检测中的应用情况。基于PCR技术的检测方法是目前果树病毒检测的主要方法,许多果树病毒的PCR检测体系已经建立。NASBA技术也已开始用于果树病毒的检测。     

秸杆栽培食用菌拮抗菌株的鉴定及混菌发酵条件的优化

从海洋泥样和食用菌发酵料中分离出了32个菌株,从中筛选出了既具有较高的纤维素酶活性又具有对青霉(Penicillium spp)、木霉(Trichoderma spp.)、根霉(Rhizopus)、曲霉(Aspergillus spp)、毛霉(Mucro)较强拮抗能力的W-4、W-10、N-14三个菌株。经鉴定W-4、W-10分别属于放线菌中黄色节杆菌(Arthrobacter flavescens)和石灰壤诺卡氏菌(Nocardia calcarea),N-14属于细菌中的乳酸乳杆菌(Lactobacillus lactis)。通过正交试验对上述3个菌株混合发酵条件进行优化,确定在温度35℃、时间36h、pH7.5、装液量60ml/250ml时为最适发酵条件。在此条件下3个菌株混合发酵液纤维素酶活力仍然很高。同时混合发酵液对青霉、曲霉、毛霉、木霉、根霉的拮抗能力分别为单菌株发酵液的1.79倍、1.36倍、1.29倍、1.04倍、1.07倍。     

Green tea polyphenols supplementation alters immunometabolism and oxidative stress in dairy cows with hyperketonemia

Peripartal cows often experience negative energy balance, and are therefore prone to suffering from metabolic diseases such as hyperketonemia, which causes financial losses in dairy farms. This study aimed to investigate the effect of green tea polyphenol (GTP) supplementation during the periparturient period on production performance, oxidative stress and immunometabolism in dairy cows with hyperketonemia. One hundred Holstein cows were assigned to GTP (0.2 g/kg DM; n = 50) or control (without GTP; n = 50) group based on body weight, previous milk yield, and parity on d 15 before expected parturition. Subsequently, 10 cows with hyperketonemia were selected from each group, according to blood β-hydroxybutyric acid (BHBA) concentration between 1.2 and 2.9 mmol/L from 2 to 3 d postpartum. All cows were fed a close-up diet and a lactation diet with or without GTP supply from 15 d prepartum until 30 d postpartum. Milk and blood samples were obtained from 20 cows selected with hyperketonemia on 10, 20, and 30 d postpartum. Compared with control cows, greater milk yield and lower somatic cell count were observed in GTP cows. The GTP group had lower concentrations of BHBA, free fatty acids, cholesterol, triglyceride, reactive oxygen species, malondialdehyde, and hydrogen peroxide, greater concentrations of glucose, lower activities of aspartate aminotransferase, alanine aminotransferase, and glutamyl transpeptidase, alongside greater activities of superoxide dismutase, glutathione peroxidase, and total antioxidant capacity. Additionally, GTP supplementation up-regulated concentrations of interleukin-6 and interleukin-10, but down-regulated concentrations of tumor necrosis factor-α, interleukin-1β, interleukin-2, interleukin-8, and interferon-γ in plasma. Greater concentrations of plasma immunoglobulin G were also detected in the GTP group. Overall, the data suggested that GTP supplementation from 15 d prepartum to 30 d postpartum improved the milk yield and health status in cows with hyperketonemia during early lactation.     

Study on Modeling for Pretreatment and Cyclic Control Stream

The characteristics of pretreatment and cyclic control stream are analysed:the form of cyclic embedded hardware circuit,the idea for designing the pretreatment program.The chart model of the system is established preliminarily.     

PROBABILISTIC EVALUATION FOR STEEL FRAME COLUMNS OF TALL BUILDINGS

Based on properties of parameters and test data of dom-estic steel,this paper analyses the reliability and partial coefficient forresistance of steel frame columns of tall builings.The result may serveas a scientific basis.It is not only for domestic steel instead of importedsteel in building tall steel structures,but also for compiling concerendstandards.     

CALCULATION OF TNE END-EFFECT FACTOR OF SYNCHRONOUS MOTOR WITH CYLINDRICAL SOLID ROTOR

This paper presents the end-effect factor of Synchronous motor with cylindricalsolid rotor offered by analysing the 3-D electromagnetic field and using the Poynting vector.To ap-ply it in an actual calculation,the end-effect factor is proved correct and applicable.     

Application of Water Quality Standand Leuel Method in Eualuazing Status Quo of Heauy-Metals Pollution in Riuers

The status quo of heauy-metals pollution in water phase of course of Long-Tan hydropower statian was eualuated by water Quality Standand Level Method. With this special method,mone objectiue and definite results were obtained.     

王家厂水库洪水实时预报方法及应用研究

考虑到流域降雨时空变化和流域地形、河道特征对流域产汇流的影响，应用新安江三水源产流模型、流域分散入流非线性汇流模型以及流域洪水实时预报校正模型，对王家厂流域洪水进行模拟，取得了较为满意的计算结果。