干热河谷印楝树干液流密度动态变化特征研究

采用TDP技术对金沙江干热河谷元谋段主要造林树种印楝的树干液流密度动态变化进行了观测,得到3点主要结论:①无论干热季节或湿润季节,印楝树干液流密度昼夜变化的规律性较强,呈明显的单峰曲线;②由干热季节转向湿润季节时,印楝树干液流密度呈下降趋势,5年生印楝在5月、7月及10月的典型晴天里的树干液流密度日变化平均值分别为12.11、7.48及6.90cm3·cm-2·h-1;③在干热季节里印楝蒸腾耗水具有相当的被动性,而在湿润季节时其蒸腾耗水则表现出主动性和平氨性,反映了印楝在不同季节里的耗水机制差异.     

基于Web内嵌Office控件的文档管理平台研究

阐述了基于web内嵌office控件的文档管理平台的基本框架结构,详细介绍了利用web-office控件实现模板的创建和文档的创建的设计原理,以及整个文档管理平台模块的详细功能实现。     

从蔬菜幼苗鉴定看我国种子检验标准语ISTA标准的差距

为了保证种子质量检测结果的可靠性和真实性，ISTA种子检验规程对每个检测项目、每个步骤都有严格的规定和详尽的说明。本文从幼苗鉴定的角度，比较了我国种子检验标准语ISTA标准的差距，并提出了相关的建议和意见。     

TCDCA对IL-1β刺激下AA大鼠成纤维样滑膜细胞中PGE2分泌的影响

[目的] 进一步探讨牛磺鹅去氧胆酸（taurochenodeoxycholic acid,TCDCA）在抗炎免疫方面的潜在调节作用。[方法] 以AA大鼠成纤维样滑膜细胞作为研究对象,采用ELISA方法检测TCDCA和IL-1β作用下AA大鼠成纤维样滑膜细胞上清液中PGE₂的含量,分析TCDCA对IL-1β刺激下AA大鼠成纤维样滑膜细胞中PGE₂分泌情况的影响。[结果] TCDCA能够对IL-1β刺激下AA大鼠纤维样滑膜细胞PGE₂的分泌产生下调作用（P<0.05）。[结论] TCDCA对IL-1β刺激下AA大鼠成纤维样滑膜细胞中PGE₂的分泌具有抑制作用,为TCDCA在兽医临床应用提供依据。     

Effects of remifentanil on monophasic action potential and transmural dispersion of repolarization in rabbit myocardium

AIM: To study the effect of remifentanil on monophasic action potential and transmural dispersion of repolarization (TDR) in the 3-layer myocardium of isolated rabbit hearts. METHODS: Adult rabbits (n=18, 2.0 ~ 2.5 kg) were used to isolate the hearts for preparing Langendorff perfusion model. The hearts were randomly divided into 3 groups after perfusion with K-H solution for 15 min: the perfusion in control group (C group) continued for 60 min; the hearts in remifentanil group (R group) were perfused with 12 μg/L remifentanil K-H solution for 60 min; the hearts in remifentanil+aminophylline group (RA group) were given 60-min perfusion of 12 μg/L K-H remifentanil+30 mg/L aminophylline. The HR and 3 layers of myocardial monophasic action potential (MAP) in the left ventricular anterior wall were recorded at time points after balanced infusion for 15 min (T₀), and continued perfusion for 15 min (T₁), 30 min (T₂) and 60 min (T₃). The monophasic action potential duration of repolarization at 90% (MAPD₉₀) and the transmural dispersion of repolarization (TDR) were calculated. The early afterdepolarization, delay afterdepolarization and arrhythmia were also observed. RESULTS: In R group, slower HR and prolonger MAPD₉₀ and TDR at T₁~T₃ were observed as compared with those at T₀ (P<0.05). R group showed slower HR and longer MAPD₉₀ and TDR than C group and RA group (P<0.05). CONCLUSION: Remifentanil slows the HR, extends the MAPD₉₀ and increases the TDR, thus being prone to induce reentry. Aminophylline makes HR faster and MAPD₉₀ shorter, thereby reducing the TDR.     

气肿疽梭菌细胞毒素CctA基因的克隆及生物信息学分析

根据GenBank中气肿疽梭菌细胞毒素CctA基因的参考序列,采用PCR方法扩增细胞毒素CctA基因。在将该基因进行克隆和测序后利用生物信息学方法,对细胞毒素CctA蛋白的理化性质、结构功能域、蛋白质二级结构和三级结构及抗原表位等重要参数进行了预测和分析。结果表明:与参考序列(JQ692583)相比,存在3处突变,核苷酸序列的同源性和氨基酸序列的同源性均为99.4%;CctA蛋白的理论等电点为8.00,不存在跨膜区和信号肽序列,二级结构以β-折叠和无规则卷曲为主,主要有4个抗原表位区域。本研究为CctA蛋白功能的深入研究提供了参考,并为气肿疽梭菌单克隆抗体的制备及合成肽疫苗的研发奠定了基础。     

基于修正RMP模式的旅游小镇主题策划——以云南省玉溪市高古楼文化旅游小镇为例

在对传统RMP模式进行客观分析的基础上,将竞争与合作变量纳入到分析框架中,提出了修正（扩展）的RMP模式,即RMCP模式。同时,结合云南省玉溪市高古楼文化旅游小镇的规划案例对RMCP模式的操作思路与方法进行了具体说明。