云南森林食品产业竞争力分析及提升路径研究

丰富的林下资源为云南经济发展提供了条件和保障,森林食品产业是林下经济产业的重要组成部分,其健康持续发展,对确保森林资源的永续利用意义重大。借助波特竞争模型构建云南森林食品主产区产业竞争力指标体系,采用层次分析法和综合指数法,计算云南森林食品主产区产业竞争力得分。结果显示,云南的森林食品产业竞争力排名在全国有一定优势,但云南的森林食品产业发展中存在着运输能力不足、产业规模偏小、政府林业投资不足以及科技支撑能力弱等问题。针对问题,对云南森林食品的产业发展路径提出了相关建议。     

Dissecting the genetic basis of maize deep-sowing tolerance by combining association mapping and gene expression analysis

Deep-sowing is an important method for avoiding drought stress in crop species,including maize.Identifying candidate genes is the groundwork for investigating the molecular mechanism underlying maize deep-sowing tolerance.This study evaluated four traits(mesocotyl length at 10 and 20 cm planting depths and seedling emergence rate on days 6 and 12) related to deep-sowing tolerance using a large maize population containing 386 inbred lines genotyped with 0.5 million high-quality single nucleotide ...     

鹿茸及鹿血中布鲁氏菌实时荧光定量PCR检测方法的建立

为建立可应用于快速检测鹿茸及鹿血中布鲁氏菌的方法,保证鹿产品的药用、食用安全,试验根据布鲁氏菌特异性基因IS711设计合成引物和探针,建立实时荧光定量PCR方法,对反应条件进行优化,并绘制标准动力学曲线,Y=-3.14X+37.62,R2=0.997.结果 表明:该方法具有良好的重复性、敏感性和特异性,组内、组间重复性...     

Experimental research on cardiac fibrosis induced by recombinant mouse interleukin-11 in mice

AIM To observe the effect of recombinant mouse interleukin-11 （rmIL-11）injected subcutaneously into mice on heart structure and function and to determine its pro-fibrotic effect. METHODS C57BL/6 mice were randomly divided into experimental group and control group.The mice in experimental group were injected subcutaneously with recombinant mouse IL-11 at the dose of 100 μg·kg^-1·d^-1 for 3 consecutive weeks, while the control group were given equal volume of normal saline in the same way. After the experiment was finished, the parameters of heart function were measured by echocardiography.The heart weight was weighed and the cardiac weight index (CWI) was calculated. HE staining and Masson's trichrome staining were performed to observe the pathological changes and the extent of myocardial fibrosis in mouse myocardia respectively, and the cardiac collagen volume fraction (CVF) was calculated. The expression levels of extracellular matrix proteins in the myocardial tissues of mice, including type Ⅰ collagen, type Ⅲ collagen and fibronectin, were determined by Western blot. RESULTS Left ventricular ejection fraction and left ventricular fraction shortening in experimental group were obviously lower than those in control group (P<0.01), however left ventricular end-diastolic diamension and left ventricular end systolic dimension were significantly higher than those in control group (P<0.05).Compared with control group, the CWI was increased (P<0.01), the myocardial arrangement was disorder, the necrosis of cardiac myocytes was increased, and excessive deposition of collagen was observed in the myocardial tissues in experimental group. Correspondingly, the CVF and protein levels of type Ⅰ collagen, type Ⅲ collagen and fibronectin in the left ventricle in experimental group were increased significantly (P<0.05). CONCLUSION Injection of rmIL-11 into the mice subcutaneously induces fibrogenesis in the heart, which implies that IL-11 is likely a novel pro-fibrotic factor.     

Dihydroartemisinin enhances radiotherapy efficiency in H22 cell tumor-bearing mice by regulating PI3K/AKT signaling pathway

AIM To study the effect of dihydroartemisinin （DHA） on the radiotherapy efficiency in hepatocellular carcinoma H22 cell tumor-bearing mice and the role of phosphatidylinositol 3-kinase （PI3K）/protein kinase B （AKT） signaling pathway in this process. METHODS A model of H22 cell tumor-bearing mice was established. The mice was divided into model group, single radiotherapy group, 5-fluorouracil （5-FU） group, and low-, medium- and high-dose DHA groups. The body weight and tumor volume in each group were measured every other day. At the end of administration, blood was collected from the tail of the mice and the animals were killed by neck removal immediately. The synergistic effect of DHA on radiotherapy was determined, and tumor growth inhibitory rate was calculated. The degree of lymphocyte transformation and natural killer （NK） cell activity were measured by MTT, the serum levels of interleukin-2 （IL-2） and IL-4 were measured by ELISA, and the protein levels of PI3K, AKT and p-AKT were determined by Western blot. RESULTS The H22 cell tumor-bearing mouse model was successfully constructed. Compared with model group, the TGT₃ （tumor growth time to reach 3 times of volume） of single radiotherapy group was remarkably increased （P<0.05）, while tumor weight, lymphocyte transformation degree, NK cell activity, IL-2 and IL-4 levels, PI3K protein level and AKT phosphorylation level were remarkably decreased （P<0.05）. Compared with single radiotherapy group, TGT₃, EF （enhancement factor）, tumor inhibitory rate, lymphocyte transformation degree, NK cell activity, IL-2 level and IL-4 level were increased with the increase in DHA dose （P<0.05）, and the PI3K protein level and AKT phosphorylation level were decreased （P<0.05）. CONCLUSION DHA may enhance the immunity of tumor-bearing mice by inhibiting the activity of PI3K/AKT signaling pathway, thereby enhancing the efficacy of radiotherapy.     

我国海岛旅游开发模式创新研究 ——以舟山群岛为例

我国海岛资源丰富,旅游开发潜力巨大.舟山群岛作为我国第一大群岛,由于缺乏科学的开发模式和管理机制,舟山群岛一些岛屿的旅游开发效果并不理想,富有吸引力的旅游岛很少.文章采用SWOT分析法,剖析了舟山群岛旅游开发的优劣势和存在的主要问题,论述了要把舟山群岛建成特色鲜明、在海内外具有较强吸引力的国际性亚热带群岛型海洋休闲旅游目的地,就必须在开发理念、开发与管理体制和模式等方面实行创新,在旅游发展空间布局上突出"一体两翼三大中心",在旅游产品开发方面重点塑造"海天佛国渔都港城-中国舟山群岛"整体旅游形象品牌,突出海洋文化和海洋休闲两个主题,精心打造海洋观光、海洋文化、海洋休闲、海鲜美食等九大旅游产品,开发好一片特色无居民海岛.     

苹果酒酵母融合子W1发酵工艺参数的优化研究

该文利用所构建的苹果酒的模糊综合评判模型,以评判结果为目标,采用响应界面法研究了苹果酒发酵的规律,建立了优质苹果酒发酵的二次多项式数学模型,同时验证了模型的有效性,并考察了接种量、初始pH值和发酵温度对苹果酒品质的影响,优化出最佳发酵工艺参数为接种量5.33%、初始pH值3.37、发酵温度22.14℃。     

茶园土壤锰的形态分布及其影响因素

茶树是富锰植物,饮茶是人体摄取锰的重要途径之一。研究茶园土壤锰的形态分布及其影响因素,对正确评价茶园土壤锰的生物有效性,调控茶叶中锰的含量,保护人类健康具有重要意义。通过对浙江、江苏和安徽13个自然茶园土壤采样,采用连续提取法将茶园土壤锰分为水溶态、交换态、碳酸盐结合态、有机结合态及残渣态,探讨了茶园土壤锰的含量、形态分布规律及其与土壤理化性质的关系。结果表明,不同茶园土壤锰含量有一定差异,随土壤pH升高和粘粒含量的增加,土壤锰全量显著增加;不同茶园土壤锰的形态分布规律略有差别。但总的规律是残渣态》交换态〉水溶态〉有机结合态〉碳酸盐结合态;茶园土壤锰的累积量对锰的各形态含量有不同程度的影响．除了水溶态和有机结合态外,交换态、碳酸盐结合态和残渣态锰均随土壤锰全量的增加而显著增加,特别是残渣态锰含量增加最明显;土壤pH、粘粒含量和阳离子交换量对茶园土壤锰的形态分布有不同程度的影响,而土壤pH是影响茶园土壤锰形态分布的重要因素。     

穗茎兼收型玉米联合收获机的发展现状与展望

随着我国农业结构的调整和畜牧业的发展,穗茎兼收型玉米联合收获机成为发展热点。为此,总结了该种机型的发展现状,简单介绍了几种较成熟机型的性能及特点。基于现状,论述了穗茎兼收型玉米联合收获机的发展趋势,并指出该种机型具有广阔的发展前景。     

聚合酶链式反应(PCR)技术检测牛奶中金黄色葡萄球菌

根据金黄色葡萄球菌特异性STAA-Aul基因序列,设计、合成了一对引物,运用聚合酶链式反应(PCR)技术从10头患有奶牛乳房炎的奶牛奶样中扩增得到8个大小为420 bp的DNA产物;阴性样品中未扩增到产物。测定了其中2个DNA片段的核酸序列,确定为金黄色葡萄球菌的STAA-Aul基因。