白蛾黑基啮小蜂种群数量变化规律的初步研究

选择徐州市林果场、云龙区和新沂市等受杨小舟蛾危害较重的不同类型杨树林分的未放蜂区(CK)和放蜂区,分别测定白蛾黑基啮小蜂在自然和人工释放2种状态下的种群数量变化。结果表明,该蜂林间自然种群数量以越冬代最高,对杨小舟蛾越冬蛹的寄生率高达(22.2±5.8)%,极显著高于第1~4代蛹(3.4%~6.4%);于越冬代和第2代害虫蛹期人工释放该蜂,释放后当代小蜂种群数量明显上升,可显著提高释放当代(越冬代和第2代)害虫蛹的寄生率,并对释放后各代害虫蛹具有一定的持续寄生效果;各代小蜂室温下的羽化进度均与相应世代寄主蛹发育进度相吻合。     

塞罕坝湿地资源现状与保护对策

文章阐述了塞罕坝湿地资源现状调查方法,并以调查资料为依据,以湿地类型、野生动植物资源状况为主要内容,分析了塞罕坝湿地资源现状和基本特征,并结合湿地所处社会环境等,提出了塞罕坝湿地的保护对策,为塞罕坝湿地保护与恢复提供基础支撑。     

基于blackboard教学平台的嵌入式信息素质教育发展策略

blackboard教学平台与嵌入式信息素质教育的结合,对图书馆员、教师和学生都具有非常重大的影响。本文通过对国外blackboard应用的现状和国内高校应用blackboard的可行性进行分析,总结出高校借助Blackboard教学平台开展嵌入式信息素质教育的发展策略。     

Effect of AIR on Inflammatory Reaction Infected by Brucella

In order to investigate the effect of AIR on inflammatory reaction infected by Brucellamelitensis (16M), the AIR domain of Tecpr1 gene of murine macrophages RAW264.7 were knocked down (I-A), overexpressed (O-A) and reversed (OA-IA). Using the chlorine fluorescein (DCFH-DA) as a probe, we detected the variation of ROS production and mitochondria distribution by confocal laser scanning microscopy. We observed the expression changes of NLRP3, ASC and Caspase-1 by qRT-PCR and the expression changes of IL-18,IL-1β and Caspase-1 in host cells by ELISA. The results showed that 16M could stimulate RAW264.7 cells to produce ROS by time-dependent pathway, and I-A group and O-A group showed more abnormal accumulation of mitochondrial. The results of qRT-PCR and ELISA suggested that it had effect on the expression levels of NLRP3, ASC,Caspase-1 and IL-18, IL-1β and Caspase-1 in cells of different groups. Those results indicated that with AIR gene deletion, the release amount of ROS changed, mitochondrial clustered abnormally, and AIR was closely related to the activation of inflammasomes and induction of inflammatory reactions.     

Advances on Detection Methods for Residues of Nitrofurans in Food

Nitrofurans are broad-spectrum antibiotics which have been widely used in food animals to the prevention and treatment of gastrointestinal infections.Due to abuse of drugs by criminals,animals could produce drug-resistant strains,which reduces the role of antibiotics.Meanwhile,excessive drugs residues in food has carcinogenic,teratogenic and mutagenic effects to the human body.In this paper,the damage and the detection methods of the residues of the nitrofurans in food are surveyed.The main detection methods include high performance liquid chromatography,liquid chromatography-mass spectrometry,liquid chromatography tandem mass spectrometry,enzyme-linked immunoassay,colloidal gold immunoassay and fluorescence immunity analysis method.The research status of various detection methods are summarized and the suggestions for further research are put forward.It is expected to provide reference for future research.     

芒果核仁油理化特性及其开发利用研究进展

芒果核仁是芒果加工的副产物,其中脂肪含量达9.0%~13.1%（干基）,从中提取的核仁油是一种优质油脂,具有较高的营养价值及开发利用前景。本文主要从芒果核仁油理化性质、脂质组成及开发利用等方面总结归纳近年来的科学研究进展,并与可可脂、类可可脂的甘油三酯组分进行对比分析,明确了芒果核仁油研究中存在的问题,并给出合理建议。     

表达鸡α干扰素重组酿酒酵母菌的构建与鉴定

通过构建鸡α干扰素真核表达载体,以获得能够表达鸡α干扰素的酵母工程菌株。首先根据GenBank公布的鸡α干扰素基因序列设计上、下游引物,以略阳乌鸡基因组DNA为模板,PCR扩增鸡α干扰素基因片段。然后,利用基因重组技术将鸡α-干扰素基因插入真核表达载体,通过菌落PCR和DNA测序确定目标载体,构建成功后转化至酿酒酵母AH109,再通过营养缺陷培养、质粒提取和PCR鉴定带JMB440-ChIFN-α载体的重组酵母菌株。最终通过酵母培养、总蛋白收集、SDS-PAGE电泳和Western blot等,确定鸡α干扰素基因能够与酵母菌株重组,并表达α干扰素,蛋白条带大小约为23ku,与预期结果一致,表明成功构建了表达鸡干扰素基因的工程酵母菌株。     

多不饱和脂肪酸的生物学功能及其在动物生产中的应用

多不饱和脂肪酸(PUFA)是一种重要的营养物质,是细胞膜磷脂的重要组成成分,在生物系统中具有广泛的生物学功能,能够调节机体的脂类代谢、免疫,具有抗癌、预防和治疗心血管疾病、促进机体生长发育以及调控基因的表达等功能。本文就多不饱和脂肪酸的来源及生物学功能进行阐述,并浅谈了多不饱和脂肪酸在动物生产中的应用现状及前景。     

香港人工林改造对林下植物自然更新 和物种多样性的早期影响

以嘉氏羊蹄甲（Bauhinia galpinii）种子为材料，开展了不同浸种催芽处理对比试验。结果表 明，相比不浸种处理，3 个浸种处理明显提高嘉氏羊蹄甲的种子发芽势和发芽率，平均苗高也明显高于不 浸种组，其中，温水(60 ℃ ) 浸种6 h 组嘉氏羊蹄甲种子发芽率最高，达74.7%，苗高生长最快。     

生物质制备合成气技术研究现状与展望

综述了生物质合成气制备技术的现状,着重讨论了生物质气化技术中气化炉类型、气化介质、气化温度和气化压力对合成气组成的影响,介绍了生物质热解油气化和生物质超临界气化制取合成气技术,以及生物质制备合成气过程中气体净化和重整变换等相关技术,分析了各种技术的特点,并展望了生物质合成气制备技术的发展方向.