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81.
82.
Relationships among milk urea-nitrogen, dietary parameters, and fecal nitrogen in commercial dairy herds
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Pipat Arunvipas John A. VanLeeuwen Ian R. Dohoo Greg P. Keefe Shelley A. Burton Kerry D. Lissemore 《Canadian journal of veterinary research》2008,72(5):449-453
Our study objective was to determine the ability of milk urea-nitrogen concentrations ([MUN]) to predict fecal nitrogen concentrations ([Fecal N]) in commercial dairy herds. A total of 83 dairy herds were each visited 3 times within 48 h after a monthly herd milk test. For each farm visit, forages were sampled for nutrient analyses, which were entered into a computerized ration evaluator, and fecal samples were taken per rectum from each of 6 cows (2 early-, 2 mid-, and 2 late-lactation). Fecal samples were pooled, mixed, and analyzed for nitrogen content. Fecal nitrogen concentrations were compared with the routinely measured nutritional parameters from the ration evaluation, and the herd average [MUN] for the previous milk test date using mixed linear regression analyses. Total protein supplied in the ration was significantly positively associated with [Fecal N], but herd average [MUN] was not associated (P > 0.10) with [Fecal N]. 相似文献
83.
Buckham Sporer KR Weber PS Burton JL Earley B Crowe MA 《Journal of animal science》2008,86(6):1325-1334
Transportation causes stress in cattle that may alter numerous physiological variables with a negative impact on production and health. The objectives of the current study were to investigate the physiological effects of truck transportation and to characterize a pattern of phenotypes in the circulation that may aid in the early identification of stress-susceptible animals that often succumb to severe respiratory disease. Thirty-six young beef bulls (Aberdeen Angus, n = 12; Friesian, n = 12; and Belgian Blue x Friesian, n = 12) were subjected to a 9-h truck transportation by road. Blood (10 mL) was collected at -24, 0, 4.5, 9.75, 14.25, 24, and 48 h relative to the initiation of transportation (0 h). Plasma was collected for the assay of various metabolic, inflammatory, and steroid variables, and total leukocyte counts were determined in whole blood at each time point. Body weight and rectal temperature were recorded at -24, 9.75, and 48 h. Transportation decreased measures of protein metabolism in the plasma, including albumin (P = 0.002), globulin (P < 0.001), urea (P = 0.006), and total protein (P < 0.001), and increased creatine kinase (P < 0.001). The energy substrate beta-hydroxybutyrate was not changed (P = 0.27). Acute phase proteins haptoglobin and fibrinogen were both decreased (P < 0.001), whereas total leukocyte counts were elevated (P = 0.002). Circulating steroid concentrations were altered, because a classical acute increase in plasma cortisol was observed with the onset of transit (P < 0.001), in association with a decrease in dehydroepiandrosterone (P = 0.07), resulting in a profound increase in cortisol:dehydroepiandrosterone ratio (P < 0.001). Plasma testosterone was decreased, whereas plasma progesterone was increased (P < 0.001) in association with the increase in cortisol (P < 0.001). There was also an effect of breed for all variables except plasma urea, creatine kinase, and testosterone, perhaps indicating that a genetic component contributed to the physiological response to transportation stress, although without any clear trend. Taken together, this profile of physiological variables in the circulation of transportation-stressed bulls may aid in the future detection of disease-susceptible cattle after transportation. Further research to validate these potential biomarkers is necessary. 相似文献
84.
DR Notter 《Reproduction in domestic animals》2008,43(S2):122-128
Maintenance of high levels of realized fertility (defined as the percentage of ewes that lamb) and appropriate levels of fecundity are critical for efficient sheep production. The optimal level of fecundity in most situations is well below the maximum attainable level and can be targeted by combining selection among and within breeds with use of an expanding array of single-gene mutations affecting ovulation rate and litter size. The heritability of litter size is approximately 0.10, allowing changes of up to 2%/year from simple mass selection. Mutations in several genes associated with the transforming growth factor β superfamily ( BMPRIB , GDF9 and sex-linked BMP15 ) can increase ovulation rates by 0.7–1.5 ova in heterozygous ewes. However, ewes that are homozygous for BMP15 or GDF9 mutations are sterile, so use of these mutations requires carefully structured breeding programmes. Improvements in fertility may be critical for autumn lambing or programmes that aspire to lamb throughout the year. Selection to improve fertility in spring matings has been successful; selected adult ewes have lambing rates of 80–85% in October and early November. The selected ewes have a dramatically reduced seasonal anestrus, and many ewes continue to cycle during spring and summer. Major genes affecting seasonal breeding have not been identified in sheep. Polymorphisms in the melatonin receptor 1a gene appear to be associated with seasonal breeding in some, but not all breeds. However, functional genomic studies of genes associated with circadian and circannual rhythms have potential to reveal additional candidate genes involved in seasonal breeding. 相似文献
85.
Sita S. Withers Daniel York Jin W. Choi Kevin D. Woolard Renee Laufer‐Amorim Ellen E. Sparger Jenna H. Burton Stephen J. McSorley Arta M. Monjazeb William J. Murphy Robert J. Canter Robert B. Rebhun 《Veterinary and comparative oncology》2019,17(3):242-252
Our lack of understanding of the immune microenvironment in canine osteosarcoma (cOSA) has limited the identification of potential immunotherapeutic targets. In particular, our ability to utilize readily available tissue from a dog's primary tumour to predict the type and extent of immune response in their pulmonary metastatic lesions is unknown. We, therefore, collected 21 matched pairs of primary tumours and pulmonary metastatic lesions from dogs with OSA and performed immunohistochemistry to quantify T‐lymphocyte (CD3), FOXP3+ cell, B‐lymphocyte (Pax‐5), and CD204+ macrophage infiltration. We found that T‐lymphocytes and FOXP3+ infiltrates in primary tumours positively correlated with that of metastatic lesions (ρ = 0.512, P = 0.038 and ρ = 0.698, P = 0.007, respectively), while a strong trend existed for CD204+ infiltrates (ρ = 0.404, P = 0.087). We also observed T‐ and B‐lymphocytes, and CD204+ macrophages to be significantly higher in a dog's pulmonary metastasis compared to their primary tumour (P = 0.018, P = 0.018, P = 0.016, respectively), while FOXP3+ cells were only significantly higher in metastases when all primary tumour and metastasis lesions were compared without pairing (P = 0.036). Together, these findings suggest that the metastatic immune microenvironment may be influenced by that of the primary cOSA, and that primary tumour immune biomarkers could potentially be applied to predict immunotherapeutic responses in gross metastatic disease. We, therefore, provide a rationale for the treatment of cOSA pulmonary metastases with immunotherapeutics that enhance the anti‐tumour activity of these immune cells, particularly in dogs with moderate to high immune cell infiltration in their primary tumours. 相似文献
86.
Ribonuclease 6 (RNase6 or RNase K6) is a protein that belongs to a superfamily thought to be the sole verte‐brate‐specific enzyme known for a wide range of physiological functions, including digestion, cytotoxicity, angiogenesis, male reproduction and host defense. In our study, 51 functional genes and 11 pseudogenes were identified from 27 Rodentia species. Intriguingly, in the 3 main lineages of rodents there were multiple RNase6s identified in all species of Ctenohystrica, whereas only a single RNase6 was observed in other Rodentia species examined except for 2 species in the mouse‐related clade. The evolutionary scenario of “birth (gene duplication) and death (gene deactivation)” and gene sorting have been demonstrated in Ctenohystrica. In addition, bursts of positive selection, diversification of isoelectric point and positive net charge have been identified in Ctenohystrica, especially at two key sites that are involved in antimicrobial function. Site Trp30 has undergone positive selection and Ile45 has changed into other residues in Group B and Group C of the Ctenohystrica. Our results demonstrated a complex and intriguing evolutionary pattern of rodent RNase6, and indicated that functional modification may have occurred, which establishes an important theoretical foundation for future functional assays in rodent RNase6. 相似文献
87.
Burton EC Miller DL Styer EL Gray MJ 《Veterinary journal (London, England : 1997)》2008,177(3):442-444
During an on-going amphibian ecology study, a free-ranging American bullfrog (Rana catesbeiana) metamorph was captured in a pitfall trap adjacent to a constructed farm pond at the Plateau Research and Education Center (PREC) on the Cumberland Plateau near Crossville, Tennessee, USA. Grossly, the right eye was approximately 50% the size of the left. Stereo and light microscopic examination revealed two granulomas within the orbit. Electron microscopic examination revealed virus particles scattered throughout one structure but mostly aggregated toward the center. Subsequent PCR and sequencing (GenBank accession Number EF175670) confirmed frog virus 3 (FV3). This represents the first report of a malformation in an anuran associated with FV3. 相似文献
88.
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90.
Arunvipas P Vanleeuwen JA Dohoo IR Leger ER Keefe GP Burton AS Lissemore KD 《Preventive veterinary medicine》2007,82(1-2):42-50
Our objective of this study was to investigate the relationship between milk urea-nitrogen concentrations ([MUN]) and first-service breeding success (FSBS) in a large number of commercial dairy herds, using various timings on [MUN]. All commercial dairy herds in Prince Edward Island on monthly milk testing (n=198) formed the sampling frame. Milk components, [MUN], 24-h milk production, and breeding data for all cows from these farms were gathered electronically from a central database. A first service between 1 June 1999 and 31 May 2000 was classified successful (FSBS=1) if it was the cow's last service and she calved 270-290 d later. Mixed logistic-regression modeling was used to determine the association between FSBS (the outcome variable) and the [MUN] closest to first service, controlling for other possible confounders and clustering effects of cows within the study herds. The final dataset included 2787 successful and 3015 unsuccessful first services. A change in [MUN] on the test closest to first service from 10 to 20 mg/dL was associated with a 13.9% reduction in the odds of FSBS (controlled for parity, milk production and days in milk). 相似文献