苯系化合物在硝酸盐还原条件下的生物降解性能

运用驯化的反硝化混合菌群进行了苯系化合物(BTEX)的厌氧降解试验.结果表明,混合菌群能够在反硝化条件下有效降解苯、甲苯、乙苯、邻二甲苯、间二甲苯和对二甲苯.BTEX的降解规律符合底物抑制的Monod模型,当初始浓度小于50mg·L^-1时,6种受试基质的厌氧降解速率顺序为:甲苯>乙苯>间二甲苯>邻二甲苯>对二甲苯>苯.整个试验过程中NO₃^-的消耗与苯、甲苯、乙苯、邻二甲苯、间二甲苯及对二甲苯生物降解之间的摩尔比分别为:9.47,9.26,1     

动物细胞分化与克隆

细胞分化是细胞行使功能的前提条件，是特定基因表达的结果。细胞分化状态的维持需要细胞外环境、细胞与细胞之间信息交流及其细胞内环境等共同作用，从而保证分化细胞染色体的特定空间结构。细胞内环境中细胞核因素的影响可以归结为后生性遗传作用，细胞质因素主要有转录后基因沉默(RNAi)和蛋白质磷酸化作用等。克隆动物的实质是细胞分化过程的逆转。克隆动物的过程及其卵母细胞质中的特殊物质破坏了维持供体细胞分化状态的各种条件，改变了分化细胞染色体的特定空间结构，对分化细胞进行了重新编程。克隆过程中卵母细胞质对体细胞的去分化过程并非完全正确，同时由于体细胞染色体可能存在的变异，导致体细胞克隆效率很低。加强对发育生物学基本问题的研究和对现有克隆动物进行详细的研究分析，并继续改进和完善现有的体细胞克隆操作程序，是提高克隆动物效率的当务之急。     

利此亚西部管道阴极保护技术

利比亚西部管道平行敷设两条不同管径的原油管道和天然气管道,通过对其阴极保护系统的预试运,发现管道的电位出现异常的波动,且波动幅度超过了允许范围,管道可能存在杂散电流的干扰。通过对管道正常运行下的IR降、全线保护电位以及同步电位和交流干扰进行测试分析,成功地解决了管道阳极接地电阻问题、无电地区的电源问题以及杂散电流干扰问题、使该管道阴极保护参数处于理想状态。     

镉(Ⅱ)-邻菲啰啉二元配合物与透明质酸相互作用的电化学性能及应用研究

本文利用线扫极谱法研究了镉(Ⅱ)-邻菲啰啉(Phen)二元络合物与透明质酸(HA)的相互作用并建立了以Cd(Ⅱ)-Phen二元配合物为电化学探针测定HA的分析测试方法。在最佳实验条件下,Cd(Ⅱ)-Phen还原峰电流的降低值同HA的浓度在4.0~110.0mg/L范围内呈良好的线性关系,线性回归方程为Δip"(nA)=3.98 C(mg/L)+30.79(n=17,γ=0.993),检测限为3.23mg/L(3σ)。将本方法应用于模拟样品中HA的测定,结果令人满意。     

细胞外环境中分化抑制物对动物胚胎干细胞克隆率的影响

介绍了饲养层、条件培养基、分化抑制因子对哺乳动物胚胎干细胞分离与克隆的影响，并探讨了白血病抑制因子影响胚胎干细胞克隆的机理。     

甘肃省草本植物白粉菌调查(一)

对甘肃省部分草本植物白粉菌进行了调查研究 ,共报道发生在甘肃省草本植物上的白粉菌12种 ;对症状、病原形态、寄主植物及采集地进行了记载 ,对生产实践中预防和控制该病害的发生有实际意义和参考价值     

Isolation and Identification of Group I Type 4 Avian Adenovirus and Analysis in Immune Effect of Fiber-2 Protein

The aim of this study was to identify pathogens, which caused pericardial effusion, hepatomegaly and bleeding at a chicken farm in Henan province, and furthermore to analyze effectiveness of immunogenic proteins of the pathogen. This study employed virus isolation, serological assays, PCR and sequencing analysis, animal experimentation, E. coli expression and protein purification, immunogenicity and challenge test and other methods. The results showed that virus was isolated in 7-day-old SPF chicken embryonated eggs, inoculated via the yolk sac route by two blind passages. Viral confirmation was carried out using PCR techniques, and showed a 900-bp-long fragment which shared a 100% homology with the Hexon gene of the serotype C4 strain. Serum neutralization results indicated that this isolate avian adenovirus was the group I type 4 avian adenovirus, named HN-ZK strain. The virus could induce CPE on primary hepatocytes of chicken embryo, and its titer was 10^7.5 TCID₅₀·0.1 mL^-1. Animal experimentation illustrated that the isolated virus caused 100% (10/10) typical symptoms and pathogenicity in 35-day-old SPF chickens. Furthermore, the DNA of the isolate virus was used as a template to express Fiber-2 fragment, with a molecular weight of 33 kDa by using the E. coli expression system, and the protein was concentrated and purified to 300 mg·mL^-1 after centrifugation and purification. SPF chickens were immunized with different doses of the purified Fiber-2 protein, and showed that a dose of 20 μg per chick was completely resistant to challenge of the virulent virus, suggesting the purified Fiber-2 protein has better immunogenicity. This study can provide data for diagnosing the hydropericardium hepatitis syndrome, and developing a genetic engineering subunit vaccine.     

Effects of Amino Acids on Inflammatory Bowel Disease and Its Signaling Pathways

The intestine is the primary organ responsible for digestion and absorption of nutrients and is frequently subjected to external environmental stimulations leading to the development of inflammatory bowel disease (IBD), which can cause serious harm to intestinal health in animals. Dietary amino acids play important roles in promoting intestinal development and maintaining intestinal health and exert diverse effects through multiple signaling pathways on the prevention and treatment of IBD, including affecting the physiological activities of intestinal epithelial cells, improving intestinal barrier function, reducing intestinal oxidative damage, regulating the production of inflammatory cytokines, and promoting the expression of endogenous antimicrobial peptides, and involved in main signaling pathways including AMP-activated kinase (AMPK), mammalian target of rapamycin (mTOR), mitogen-activated protein kinase (MAPK), Toll-like receptors (TLRs), nucleotide binding oligomerization domain (NOD)/nuclear factor kappa-B (NF-κB). In this review, basic characteristics of IBD, effects and involved signaling pathways of the amino acids on IBD, and effects and applications of amino acids in maintaining intestinal health in livestock and poultry production were reviewed, so as to provide effective clues and strategies for dietary nutrients in the prevention and treatment of IBD.     

牛肌生成抑制素基因酵母双杂交诱饵载体的构建及鉴定

采用RT- PCR技术扩增牛肌生成抑制素基因成熟片段,并克隆入质粒pSos中,测序鉴定后获得酵母双杂交诱饵重组质粒pSos-MSTN.将诱饵重组质粒与对照质粒转化入酵母菌cdc25Hα中.结果表明:该段基因表达的蛋白对酵母菌cdc25Hα无毒性和无自激活作用,这说明可以用此酵母双杂交系统对牛肌生成抑制素进行研究.     

山药蛋糕的微波生产工艺和品质优化

为生产出既具有山药特有风味,又具有一定保健功能的山药蛋糕,以山药作为蛋糕生产的原料,研究山药蛋糕微波生产工艺。通过单因素试验和正交试验探讨鸡蛋、山药、泡打粉的添加量和微波时间对山药蛋糕品质的影响。结果表明,山药蛋糕最佳配方为低筋面粉200g,山药泥28g,鸡蛋520g,绵白糖140g,泡打粉9g,奶粉30g,食用油60mL,食盐2g,香兰素1g,羧甲基纤维素1g,单甘酯0.6g;微波焙烤采用800W功率,时间为2min,该工艺条件下,蛋糕口感香甜松软,整体起发均匀、柔和,富有弹性,定型情况良好,色泽、滋味等感官性状佳,整体品质良好。