彩叶植物金叶莸的组织培养及植株再生研究

对彩叶植物金叶莸进行了初代培养、继代培养、生根培养及驯化移栽等试验.结果表明:初代培养的最佳培养基为MS 6-BA 1.5 mg/L NAA 0.5 mg/L;继代培养的最佳培养基为MS BA 1 mg/L NAA 0.5 mg/L;生根培养的最佳培养基为1/2 MS NAA 0.5 mg/L或1/2 MS NAA 0.25 mg/L IBA 0.25 mg/L;驯化移栽的最佳基质为珍珠岩 腐叶土(1∶1).     

动物科学专业本科人才培养目标之我见

动物科学专业本科人才培养目标是确定动物科学专业本科课程教学方案的依据,是培养什么质量的动物科学专业本科人才的指导思想。动物科学专业的人才培养目标应包含如下几个重要方面。首先,动物科学专业人才培养目标应着眼于人文精神和合作精神的培养。其二,应注重基础课的教学,使学生获得终生学习的能力。其三,应促进学生的主动性思维,而不是让学生被动地接受知识的传授。其四,应提高学生的实践动手能力。最后,应在本科教学阶段继续提高动物科学专业本科生的语言技能。本文对如上方面逐一进行了阐述。     

黑龙江省猫旋毛虫18S rRNA基因分子克隆及序列分析

本文利用GenBank中发表的(Trichinella spiralis)18S rRNA序列为参考设计引物,对分离自黑龙江省猫体内的旋毛虫及本地毛形线虫(Trichinella nativa)的18S rRNA基因进行扩增,克隆后测序,序列分析结果表明:猫旋毛虫与旋毛形线虫基因同源性更高。     

哺乳动物硒蛋白的研究进展

硒是哺乳动物和人必需的微量元素，其生物学功能主要是以硒蛋白的形式表现的。近年来，关于硒蛋白作为饲料添加剂方面的报道层出不穷。作者就硒蛋白概况、分类、几种重要硒蛋白及其生物学功能、硒蛋白的合成及调控作一综述。     

不同盐分胁迫对高冰草种子发芽的影响

对高冰草种子在4种盐分NaCl、Na2SO4、Na2CO3、NaHCO3的8个不同浓度(0.2%、0.4%、0.6%、0.8%、1.0%、1.2%、1.4%、1.6%)的胁迫下进行发芽试验,分别观测其发芽情况,胚根和幼苗的生长。结果表明:高冰草种子的发芽率随盐分浓度的增加而降低。其耐盐顺序为Na2SO4>NaCl>NaHCO3>Na2CO3。     

紫色辣椒新品种‘紫燕1号’

 ‘紫燕 1 号’为辣味中等彩色辣椒一代杂种。利用国内优良自交系与从国外紫色甜椒多代自交优良株系杂交而成。该品种早熟, 从定植至采收商品椒约42 d; 抗性强, 耐低温弱光; 果实牛角形, 鲜果紫色, 生物学成熟果深红色, 鲜椒产量39 000～45 000kg/hm²。适于春早熟和秋延后保护地栽培。     

Increased expression and possible roles of nicotinamide N-methyltransferase in pancreatic islets of STZ-induced diabetic monkeys

AIM: To verify and localize the expression of nicotinamide N-methyltransferase (NNMT) in pancreas of streptozotocin(STZ)-induced diabetic monkeys and understand its important role in β-cell destruction in the pathogenesis of diabetes. METHODS: Through an olig-microarray gene chip, NNMT was identified as the most obviously up-regulated genes in pancreas of STZ-induced diabetic monkeys versus controls. Semiquantitative RT-PCR and Western blotting were performed to verify the differential expression at mRNA and protein level respectively. Then the cellular localization of NNMT expression within pancreas was identified by immunohistochemical(IHC) staining.RESULTS: An obvious high expression of NNMT at both mRNA and protein levels was shown in pancreas of STZ-induced diabetic monkeys compared to that of controls. Further localization of the protein by IHC staining in pancreas specimens showed that its altered expression was restricted to central islets, most of which were β cells.CONCLUSION: Expression of NNMT is increased in islets of STZ- induced diabetic monkeys, which infers that NNMT might participate in the process of β cell damage in diabetes probably through the mechanism of energy metabolism disturbance.     

Neuropeptide Y induced redistribution of intracellular free calcium in rat cardiomyocytes

AIM: To investigate the effect of neuropeptide Y (NPY) on intracellular free calcium(［Ca²⁺］_i) and Ca²⁺ sarcoplasmic reticulum of cardiomyocytes in rats.METHODS: Cardiomyocytes of neonatal Sprague-Dawley rats were incubated with NPY at concentration of 100 nmol/L for 24 h. Fluorescent indicator Fluo-4 AM was used to detect ［Ca²⁺］_i and Fluo-5N AM was used to detect Ca²⁺ in sarcoplasmic reticulum (SR). Calcium image was recorded by laser scanning confocal microscope. The SR Ca²⁺ load was estimated by caffeine-induced Ca2+ transient (CCT). RESULTS: 24 h after incubation with NPY, compared with control group, the concentration of ［Ca²⁺］_i was significantly elevated (P<0.05), and the concentration of free Ca2+ in SR (［Ca²⁺］_SR) was significantly decreased (P<0.05), and the peak of CCT was attenuated.CONCLUSION: Stimulation with NPY for 24 h causes redistribution of free calcium in rat cardiomyocytes, namely the elevation in ［Ca²⁺］_i and decline in ［Ca²⁺］_SR.     

Thrombolytic properties of a conjugate of liposomal urokinase with a monoclonal antibody specific for cross-linked fibrin in the model of rabbit artery thrombosis

AIM: To observe the targeting thrombolytic effect of a monoclonal antibody specific for cross-linked fibrin connected with liposomal urokinase（UK） in the model of rabbit artery thrombosis.METHODS: Preparation of thrombolytic solutions: with the method of controllable dialysis eradicator in liposomat,empty liposomes,liposomally entrapped urokinase and liposomally entrapped urokinase linked with D-dimer antibody were made.Experiment in vivo: a rabbit thrombosis model of abdominal aorta was induced by ferric chloride.When the blood pressure fall to the lowest,5 different solutions were separately imported (PBS,maximal-level UK,Ab/Lip/UK,Ab/UK-Lip,Ab-UK-Lip) and observed for 40 min continuously.RESULTS: The varieties of 5 groups blood pressures were analyzed with q-test.Significant differences were observed among Ab-UK-Lip group,maximal-level UK group and others (P<0.01).There were marked differences between Ab-UK-Lip group and others in wet weight of thrombuses (P<0.05).However,the quantity of UK in Ab-UK-Lip group was 1/3 of that in maximal-level of UK group.CONCLUSION: The thrombolytic effect on Ab-UK-Lip group is similar to that on maximal-level of UK group,but the dosage of UK is less,and the side-effect is light.This shows,as a homing-device of liposomal urokinase,the D-dimer antibody has targeting effect and the agent of Ab-UK-Lip will be attached importance by-and-by.     

万寿菊细菌性叶斑病药剂防治试验

通过室内抑菌筛选和田间小区及生产试验,从当前防治细菌性病害的药剂中,筛选出对万寿菊细菌性叶斑病防治效果较好的3种药剂及最佳使用浓度:90%新植霉素150mg/L,细菌清200 mg/L和72%农用链霉素150 mg/L,其防效分别为73.6%,72.7%和70.7%,且药效稳定,增产效果显著.