两种土壤碱解氮测定方法的比较

测量土壤碱解氮含量过去一般使用碱解扩散吸收法,试验研究旨在使用德国GERHARD凯氏定氮蒸馏法测定土壤碱解氮,对凯氏定氮蒸馏法进行了改进,确定了合适的蒸馏时间和蒸馏效率,与传统碱解扩散法相比,测量结果更准确,紧密度更高,操作简便,节省时间,为土壤碱解氮含量测定提供了一种准确、快速、高效的测定方法。     

兰州市园林绿化用土及绿地土壤质量管理现状与对策

通过研究分析表明：兰州市园林绿化用土紧缺，绿地土壤存在密实度高、有机质含量低、污染严重、土源短缺、养护管理缺乏科学性等问题，必须提高土壤改良的科学性和管理的有效性，确保兰州市园林绿化事业的可持续发展。     

The role of TF-1 cell apoptosis-related gene 19 in systemic lupus erythematosus

AIM:To clarify the role of new apoptosis-related gene, TF-1 cell apoptosis-related gene 19(TFAR19), in the pathogenesis of systemic lupus erythematosis (SLE) and the relationship between TFAR19 and SLE.METHODS:DNA Ladder detection, Western blotting, immunological fluorescence method, ELISA and so on were used to test if ultraviolet B(UVB) could induce HaCaT cell apoptosis and TFAR19 expression.RESULTS:HaCaT cell apoptosis could be detected after 24 hours of 30 mj/cm² UVB irradiation. Also, we found that in active SLE patients, the TFAR19 antibody was increased, but not significant compared to the normal control.CONCLUSION:TFAR 19 is involved in the process of UVB induced ketatinocyte line HaCaT apoptosis and SLE pathogenesis.     

水分胁迫下华山松抗腐烂病的研究

用不同浓度的聚乙二醇（PEG6000）对华山松幼苗进行诱导水分胁迫，树皮的相对膨胀度（RT值）随PEG浓度的增大而下降；且树皮内的酚类物质含量、多酚氧酶（PPO）活性和苯丙氨酸解氨酶（PAL）活性均有所降低，过氧化物酶（POD）活性升高。说明水分状况直接影响着华山松的抗病性。4种酚类物质抑菌试验表明，一定浓度下酚类物质对腐烂病病原菌松黑腐皮壳（Valsa pirni Fr.)菌丝体生长有抑制作用，以香草醛和邻苯二酚抑菌能力最强。     

Nasopharyngeal cryptococcosis

Naturally occurring cryptococcosis in five cats, a dog and a koala is described. Involvement of the nasopharynx was documented in all patients, and nasopharyngeal mass lesions accounted for the major presenting complaints in four. Signs referable to nasopharyngeal disease included snoring, stertor, inspiratory dyspnoea and aerophagia. Diagnoses were made by caudal rhinoscopy using a retroverted flexible endoscope, vigorous orthograde flushing with saline, or at necropsy. Concurrent cryptococcal rhinitis was present in all cases, although involvement appeared limited to the caudal nasal cavity in most cases. Typical signs of nasal cavity disease, such as sneezing and nasal discharge, were often absent. Treatment of nasopharyngeal cryptococcosis should include physical dislodgement or debulking of lesion(s) to provide immediate alleviation of upper airway obstruction, followed by systemic antifungal therapy to eliminate residual infection from the nasal cavity. Infections caused by Cryptococcus neoformans var gattii accounted for a disproportionately large number of these cases.     

Expression of Gab2 in human osteosarcoma U2-OS cells

AIM: To investigate the expression of Grb2-associated binding protein 2 (Gab2) in human osteosarcoma cells and its relationship with the invasion and metastases of human osteosarcoma cells. METHODS: The technique of small RNA interference was used to transfect human osteosarcoma U2-OS cell lines. Western blotting and RT-PCR were used to detect the protein and mRNA expression of Gab2 in transfected U2-OS cells. After transfection, through chemotaxis and invasion assays in vitro, the cell migration and invasion abilities were detected. RESULTS: After transfection, the expression of Gab2 at mRNA and protein levels in Gab2 siRNA transfected cells (SiGab2/U2-OS) was lower than that in scrambled siRNA transfected cells (Scr/U2-OS) and U2-OS cells. After stimulation with epidermal growth factor (EGF) at concentration of 10 μg/L, the migration SiGab2/U2-OS cells was significantly less than Scr/U2-OS cells and U2-OS cells (P<0.01). The number of invasion cells of SiGab2/U2-OS group was significantly lower than the other 2 control groups (P<0.01). CONCLUSION: Inhibition of Gab2 expression obviously attenuates the migration and invasion abilities of human osteosarcoma U2-OS cell line.     

转MvNHX1和MvP5CS基因棉花耐盐抗旱性比较与育种价值分析

通过对花粉管通道法获得的T7转MvNHX1基因的10个棉花株系和转MvP5CS基因的3个棉花株系与对照非转基因棉花D5在温室内盐和干旱胁迫下的发芽率、生理生化指标以及田间花期干旱胁迫下农艺性状和纤维品质进行分析发现:温室内盐和干旱胁迫后,转基因棉花叶片叶绿素含量、脯氨酸含量均高于对照,而丙二醛含量低于对照;田间花期干旱胁迫下,2种转基因植株果枝数、有效果枝数、铃数、有效铃数、铃重、子棉、皮棉、衣分、子指和衣指均高于对照,说明转MvNHX1基因和转MvP5CS基因植株在干旱逆境下的产量高于非转基因;经花期干旱胁迫后,2种转基因棉花的纤维断裂伸长率、短纤维率、马克隆值和纺纱一致性指数优于非转基因棉花D5。综合分析表明:2种转基因棉花的耐盐抗旱性均有提高,其中转MvNHX1基因棉花的耐盐性优于转MvP5CS基因棉花植株,而转MvP5CS基因棉花植株的抗旱性优于转MvNHX1基因棉花植株。     

转基因抗虫棉Bt基因的遗传连锁分析

对中美两国所获得的转基因抗虫棉品种GK— 1 2、新棉 33B分别进行了 Bt基因的连锁测验。分析表明两个品种的试验结果相似 ,Bt基因未整合在棉花第 I、II、III、IV、V、VI、VII、IX、X、XI、XII、XIII、XVII连锁群及 cu、pg、gl1等基因所涉及的染色体上。阐明了 Bt基因染色体图谱定位的复杂性 ,并提出以原位杂交技术实现 Bt基因在棉花染色体组上的物理标图     

高强纤维双价抗虫杂交棉苏杂3号品种特性分析

分析了高强纤维双价转基因抗虫杂交棉苏杂3号的品种特性,结果显示:①苏杂3号纤维强力高、品质优,HVICC纤维长度30.3 mm、比强度34.3 cN/tex、整齐度85.2%、伸长率7.1%、马克隆值4.6、反射率77%、黄度8.1、纺纱均匀性指数158;②苏杂3号综合抗病虫性能好,抗棉铃虫、高抗红铃虫、高耐枯萎病、耐黄萎病;③苏杂3号产量水平高,子棉单产3 359.4 kg/hm2,比对照泗棉3号增产15.14%;皮棉单产1 304.7 kg/hm2,比对照增产0.37%。苏杂3号集高强纤维、高产、抗虫于一体,是新一类型的高品质国产双价抗虫杂交棉。抗虫蛋白试剂条检测显示,苏杂3号具有B t毒蛋白的高阳性表达。PCR检测结果表明,苏杂3号含有中国合成构建的外源B t CpTI双价杀虫基因片段。     

雏鸡中脑视顶盖I层细胞构筑研究

为进一步阐明鸟类视觉通路的结构特征,试验采用Golgi-Cox法,灌流固定后尼氏体(Nissl bodies)染色和羰花青荧光染料DiI(1,1-’dioctadecyl-3,3,3’,3-t’etramethylindocarbo-cyanine perchlorate)逆向神经标记技术,对雏鸡视顶盖I层的厚度和该层的细胞形态、大小进行了观察研究和数理统计。结果表明,I层细胞多数呈梭形,还有少数呈圆形、椭圆形、锥形、三角形和不规则形状等。I层外侧部较背侧部和腹侧部厚。按照胞体面积大小将细胞分为4类:巨细胞、大细胞、中细胞和小细胞,I层背侧、腹侧和外侧以巨细胞最少,其中背侧、腹侧以小细胞为主,外侧以中细胞数量最多。I细胞具有2~6个主树突,其中有2个主树突的细胞最多(60.7%,n=128)。