黑稻CHS基因外显子Ⅱ部分序列遗传变异分析

以汉中栽培2种黑稻(黑丰,黑米B)和1种白稻(香粘)为材料,根据水稻查尔酮合成酶(chalcone synthase,CHS)基因DNA序列的保守区域设计3对引物,进行PCR扩增,获得CHS外显子Ⅱ部分序列,测序并与籼稻CHS基因(X 89589.1)比对,发现有13处SNP,这些SNP可能是黑稻种子黄酮类物质含量差异的重要原因之一.稻和其他植物CHS基因外显子Ⅱ序列的进化分析表明,CHS基因虽然整体上反映进化关系,但由于不同基因的进化历史不同,因此单独用CHS基因的进化树来反映物种的进化关系是不确切的,而应尽量选择多位点进行研究.研究结果为高黄酮类物质含量的黑稻育种和黑稻CHS基因在分子系统分类方面提供了必要数据.     

习水国家级自然保护区四川大头茶群落研究

以习水国家级自然保护区天然四川大头茶群落为调查研究对象,分析了群落的物种组成、外貌特征和结构特征,定量计测了乔木层主要树种的群落特征指标.结果表明,习水自然保护区四川大头茶群落植物共有31科49属53种;垂直结构明显,分为乔木层、灌木层、草本层,乔木层的物种多样性指数较高,物种丰富;四川大头茶在群落中处于优势种地位;群落结构趋于稳定.     

Removal of Dmethyl Phthalate from Water with Enhanced Coagulation Technology

The removal of Phthalate Esters (PAEs) from water was measured by using enhanced coagulation technology.Cationic coagulant poly-diallyl-dimethyl-ammonium-chloride (PDMDAAC) and polyacrylamide (CPAM) were employed to remove the dimethyl phthalate (DMP),and the residual concentration of DMP is determined by the high performance liquid chromatography (HPLC).During the coagulation/flocculation process,parameters such as coagulant dosage,pH value,and sedimentation time are investigated.The results show the use of coagulant PDMDAAC/CPAM together has better coagulation effect than PDMDAAC.DMP removal rate is 99.87% when initial DMP concentration is 0.50 mg/L,the addition of a PDMDAAC dosage 50 mg/L,the addition of a CPAM dosage 2.5 mg/L,pH value 6.0 and sedimentation time 3 hours.Therefore,the removal of PAEs by coagulation is effective,and the application of coagulation by PDMDAAC/CPAM in water supply will enhance the removal of PAEs and at the same time reduce the water turbidity.     

Improvement of circulatory circle design method forautomobile hydraulic torque converters

A design method to reduce the circulatory circle axle size for automobile hydraulic torque converters is proposed. According to the beam flow theory, the variety of the axle size has little effect on the characteristics of torque converters. Based on analyzing traditional design method, it’s found that the basic reason for oversize of axle is the unreasonable semi-empirical expression of the first and third arc radius. An improved design semi-empirical expression is proposed for passage car torque converters based on the analysis of several foreign mature torque converters, and an improved design method is established. The improved method is used to design a torque converter, and the results show that the circulation circle axle size reduces about 26% in the condition of keeping the same characteristics of the traditional method. It means the improved design method can better meet the design requirement of automobile torque converters.     

带压电层圆柱形杆中波的传播

基于Hamilton原理的矩阵形式,得到带压电层圆柱形杆中波传播的基本方程式;采用幂级数的不同幂次近似方法表达杆件中的位移函数;通过变分求得特征方程式;进而求得弹性简谐波在带压电层的圆柱杆件中传播时的频散关系和位移场.通过实例计算,得到了压电圆柱杆件中波传播的频散关系,讨论了压电层对波传播的影响.计算结果表明,这种方法能够得到较好的收敛结果.     

黑龙江省乡土杨树与美洲黑杨杂交新品种的选育技术

通过试验和评比,选出了生长性、抗性强的4个杂种无性系,其无性系为苇河大青杨×盖县3、带岭大青杨×山海关2、方正小叶杨×盖县2、苇河大青杨×盖县2.     

人工植被坡面产流问题研究进展

随着山坡水文学的发展，人工植被坡面产流问题的研究在水文学领域中具有十分重要的意义，且受到越来越广泛的重视．分析了人工植被坡面径流的研究现状，并简要论述了坡面产流研究的若干成果及未来展望。     

肉牛早孕因子（EPF）的活性检测及分离纯化

【目的】检测怀孕肉牛早孕因子(Early pregnancy factor,EPF)的活性,并对其进行分离纯化,以期为EPF快速检测方法的建立提供依据。【方法】采集妊娠1~9个月的肉牛血样,并进行妊娠诊断,通过玫瑰花环抑制试验(Rosette inhibition test,RIT)检测其早孕因子的活性。对有活性的血样依次进行DEAE-52离子交换层析、ConASepharose 4B亲和层析和Heparin Sepharose Cl 6B亲和层析,分离纯化EPF,对纯化产物进行SDS-PAGE检测,并测定其等电点。【结果】怀孕牛和未怀孕牛血清的玫瑰花环抑制滴度差异显著(P<0.05)。玫瑰花环抑制试验显示,纯化出的EPF具有活性,其分子质量为53.6 ku,等电点为6.8。【结论】利用玫瑰花环抑制试验检测早孕因子活性,可以鉴定肉牛的妊娠状态;利用层析的方法可以分离出早孕因子的活性蛋白。     

团头鲂细菌性败血症免疫研究

将温和气单胞菌(Aeromonas sobria)复壮，在28℃条件下加福尔马林灭活48h，制备成灭活苗。用该疫苗免疫团头鲂，取免疫后第10d，20d，30d的血清做凝集抗体试验，最高凝集抗体效价达1：32～128，对照组为1：4～8；免疫鱼的抗感染保护力达70％。     

高校图书馆网络信息安全的风险与对策

病毒、黑客、内部的误用和滥用等风险，威胁着图书馆网络信息安全。防御这些风险，应从管理和技术两个层面进行，遵循“管理为先，技术为重”的原则，将图书馆网络安全防御技术应用于整个网络中。全局安全网络应具有自动修复功能，对用户接入需求进行有效控制，并建立入侵检测系统对网络安全隐患进行预防。