A hybrid swarm population of Pinus densiflora × P. sylvestris inferred from sequence analysis of chloroplast DNA and morphological characters

To confirm a hybrid swarm population of Pinus densiflora × P. sylvestris in Jilin, China, we used needles and seeds from P. densiflora, P. sylvestris, and P. densiflora × P. sylvestris collected from natural stands or experimental stations to study whether shoot apex morphology of 4- year old seedlings can be correlated with the sequence of a chloroplast DNA simple sequence repeat marker (cpDNA SSRs). Total genomic DNA was extracted and subjected to sequence analysis of the pine cpDNA SSR marker Pt15169. Results show that morphological characters from 4-year old seedlings did not correlate with sequence variants of this marker. Marker haplotypes from all P. sylvestris trees had a CTAT element that was absent from all sampled P. densiflora trees. However, both haplotype classes involving this insertion/deletion element were found in a P. densiflora × P. sylvestris population and its seedling progeny. It was concluded that the P. densiflora × P. sylvestris accessions sampled from Jilin, China resulted from bi-directional crosses, as evidenced by both species’ cpDNA haplotypes within the hybrid swarm population.     

Modulus of elasticity declines with decreasing planting density for loblolly pine (Pinus taeda) plantations

Context

Loblolly pine is often grown in intensively managed plantations for wood production. In order to fully evaluate the effects of management practices on wood quality and ultimately value, it is necessary to relate mechanical properties to management practices.

Aims

The aim of this study was to evaluate the effect of planting density on mechanical properties of lumber recovered from loblolly pine trees from a 27-year-old spacing trial and develop prediction equations for modulus of elasticity and modulus of rupture from stand, tree, and board characteristics.

Methods

Regression methods were applied to sample trees from three planting densities (2,989, 1,682, and 746 trees ha^?1) and used to relate mechanical properties of lumber extracted from the trees to stand, tree, and board characteristics.

Results

Initial planting density was found to be correlated with modulus of elasticity and, to a lesser extent, with modulus of rupture. Including board characteristics and utilizing the visual grade and board position as regressors produced improved prediction equations.

Conclusions

The mean modulus of elasticity declines with decreasing planting density while the variability increases, suggesting that planting density is a surrogate for frequency and size of knots. Thus, lower planting densities, while producing more lumber, may produce proportionally fewer boards of greater modulus of elasticity than higher planting densities.     

Seasonal variation of soil respiration rates in a secondary forest and agroforestry systems

Agroforestry systems are widely practiced in tropical forests to recover degraded and deforested areas and also to balance the global carbon budget. However, our understanding of difference in soil respiration rates between agroforestry and natural forest systems is very limited. This study compared the seasonal variations in soil respiration rates in relation to fine root biomass, microbial biomass, and soil organic carbon between a secondary forest and two agroforestry systems dominated by Gmelina arborea and Dipterocarps in the Philippines during the dry and the wet seasons. The secondary forest had significantly higher (p < 0.05) soil respiration rate, fine root biomass and soil organic matter than the agroforestry systems in the dry season. However, in the wet season, soil respiration and soil organic matter in the G. arborea dominated agroforestry system were as high as in the secondary forest. Whereas soil respiration was generally higher in the wet than in the dry season, there were no differences in fine root biomass, microbial biomass and soil organic matter between the two seasons. Soil respiration rate correlated positively and significantly with fine root biomass, microbial biomass, and soil organic C in all three sites. The results of this study indicate, to some degree, that different land use management practices have different effects on fine root biomass, microbial biomass and soil organic C which may affect soil respiration as well. Therefore, when introducing agroforestry system, a proper choice of species and management techniques which are similar to natural forest is recommended.     

Up‐Regulation of Insulin‐Like Growth Factor I and Uteroglobin in In Vivo‐Developed Parthenogenetic Embryos

Parthenote embryos are being considered as an alternative source of embryonic stem cells. However, as there is still a dearth of knowledge of this kind of embryos, a better understanding of their biology is needed for their application. In this work, we studied the differences and similarities between parthenotes and normal embryos at the blastocyst stage in vivo developed. We analysed the expression of factor OCT‐4, vascular endothelial growth factor (VEGF), insulin‐like growth factor I (IGF‐I) and uteroglobin (UG) by real‐time PCR. To do so, oocytes were recovered and after activation procedure were transferred by ventral middle laparoscopy to receptive does to undergo completely in vivo development. Does were slaughtered 6 days post‐ovulation induction, and parthenote and normal embryos were recovered for mRNA expression analysis. Our results reported that parthenotes and normal embryos showed similar mRNA expression for OCT‐4 and VEGF. However, IGF‐I and UG showed to be over‐expressed in parthenote embryos. Thus, our study highlights that despite the in vivo development of parthenotes, they still seem to have an altered expression and, therefore, to be different to normal embryos. The altered expression pattern of parthenote embryos suggests that these embryos should be studied carefully before future application.     

Effects of binder fibers and bonding processes on PET hollow fiber nonwovens for automotive cushion materials

In this study, nonwoven fabrics were developed for the replacement of polyurethane foams in car interiors, in particular, cushioning materials for car seats. Polyethylene terephthalate (PET) hollow fibers and two types of bicomponent binder fibers were used to manufacture automotive nonwovens by carding processes and then post-bonding processes, such as needle punching or thermal bonding. The physical and mechanical properties of nonwovens were thoroughly investigated with respect to the effects of binder fibers and bonding processes. The tensile strength and elongation for nonwovens were found to be significantly improved by combined needle punching and thermal bonding processes. In addition, the nonwoven cushioning materials were characterized in terms of hardness, support factors, and compressive and ball rebound resilience. The nonwovens showed greater hardness than the flexible PU foam. However, support factors over 2.8 for the nonwovens indicated improved seating comfort, along with better seating characteristics of greater resilience and air permeability in comparison with the PU foam.