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71.
The epidemic simulator EPIMUL was modified and used to study how induced resistance affected the development of epidemics in host mixtures. In the model, induced resistance resulted from the interaction of host tissue with avirulent spores and caused a reduction in the efficacy of virulent spores deposited afterwards. We denned three parameters to describe induced resistance: the level of protection, defined as the magnitude of reduction in the virulent spore efficacy for infecting host tissue; the host surface area protected by an interaction with one avirulent spore; and the duration of protection of the host tissue, in days. In our simulations, induced resistance slowed the epidemics and gave better disease control in the mixtures, even if protection lasted for only 2 days. The disease reduction in the mixture attributable to induced resistance was approximately proportional to the level of protection. The effect of induced resistance increased as the protected area increased. Epidemics were virtually unaffected by induced resistance restricted to the infection site, but the effect of induced resistance initially increased rapidly as larger areas were protected. There was little further gain as the protected area increased from 2·6% to 26%. The influence of induced resistance was reduced when the interactions between virulent and avirulent pathogens were reduced. 相似文献
72.
Fungi known to produce lytic enzymes were used in an attempt to control wilt of tomato caused by Fusarium oxysporum f. sp. lycopersici (FOL). Some of the fungal species (Penicillium oxalicum, Penicillium purpurogenum and Aspergillus nidulans) damaged hyphae of FOL in vitro and reduced the numbers of microconidia in the soil. Treatments with fungi did not result in a reduction in either chlamydospores of FOL in soil or populations of FOL in the rhizosphere of tomato. P. oxalicum was the most effective agent of biocontrol, and it reduced disease severity in both non-autoclaved (20% decrease) and sterile soil. In sterile soil, P. oxalicum reduced disease with different levels of severity (27% decrease at high levels and 50% decrease at low levels). Disease control by A. nidulans and P purpurogenum was only achieved when disease severity was low in sterile soil (55% and 45%, respectively). 相似文献
73.
Ergot disease spread rapidly in Zimbabwe amongst replicated plots of male-sterile sorghum A-lines, from a group of centrally situated and precociously inoculated plants. Prominent secondary conidiation by the pathogen, Claviceps africana , on the surface of exuded honeydew provided airborne spores which were trapped in a Burkard continuous spore trap and showed diurnal peaks of concentration in air close to the primary source of inoculum. The rate of disease spread ( r =0·2; range 0·14–0·58) closely matched that recorded for other plant pathogens such as Phytophthora infestans and Puccinia graminis tritici , and it is concluded that the characteristic secondary conidia of C. africana were the principal epidemiological agents within the experimental area. Ergot spread by windborne secondary conidia has significant epidemiological and economic implications for sorghum hybrid breeding in southern Africa. 相似文献
74.
Racial diversity and complexity in regional populations of Erysiphe graminis f.sp. hordei in France over a 5-year period 总被引:2,自引:3,他引:2
The evolution of race patterns in three French regional populations of the barley powdery mildew pathogen Erysiphe graminis f.sp. hordei over a 5-year period showed rapid adaptation to newly introduced host resistance genes. In all three regions, the main change consisted of the replacement of initially abundant races by pathotypes differing markedly from them by their virulence gene combinations. This explained the increase in diversity during the first 3 years of the survey, when the second group of pathotypes became more common in the populations, and its subsequent decrease due to the decline of the first group of races. The mean number of virulence genes per isolate did not vary noticeably over time in the three populations, remaining at about four out of 12 genes tested. However, the distribution of the isolates into virulence complexity classes was greatly modified, fitting a binomial distribution by the end of the study, although significant deviations were apparent in the first 2 years (1986 and 1987). The data indicate that selection, migration and recombination are the most important factors shaping race structure and evolution in powdery mildew populations, and that mutation is of limited significance. No convincing evidence was obtained for the existence of stabilizing selection sensu Vanderplank as the mechanism limiting virulence complexity. Implications regarding spatial and temporal deployment of race-specific resistance genes to control powdery mildew are discussed. 相似文献
75.
Harvey RB Young CR Ziprin RL Hume ME Genovese KJ Anderson RC Droleskey RE Stanker LH Nisbet DJ 《Journal of the American Veterinary Medical Association》1999,215(11):1601-1604
OBJECTIVE: To enumerate the prevalence of Campylobacter isolates in the intestinal tract of market-weight swine raised in an integrated swine operation in Texas. SAMPLE POPULATION: Samples of cecal contents were collected from 595 pigs (mean body weight, 110 kg [242 lb]) at time of slaughter. Pigs were off-spring of Yorkshire-Landrace sows and Duroc or Hampshire boars. Pigs originated from 4 farrow-to-finish farms. PROCEDURE: During a 9-month period, visits were made to a slaughter plant to remove cecal contents from market-weight hogs. Samples were obtained from 50 pigs/visit from designated farms so that samples were obtained 3 times from pigs of each of 4 farms. Isolation of Campylobacter spp was accomplished by use of enrichment broth and restrictive media, using microaerophilic conditions. RESULTS: Campylobacter spp were isolated from 70 to 100% of the pigs, depending on the farm and the date the samples were collected. Campylobacter coli was isolated from 20 to 100% (mean, 60%) of samples, and C jejuni was isolated from 0 to 76% (mean, 31%) of samples. Campylobacter lari was isolated from 2 pigs. Concentrations of C coli or C jejuni ranged from 10(3) to 10(7) colony-forming units/g of cecal content. CONCLUSIONS AND CLINICAL RELEVANCE: Campylobacter coli generally is accepted as a common inhabitant of the intestinal tract of swine. However, analysis of results of this study suggests that a relatively high prevalence of C jejuni may be found in pigs raised on specific farms. 相似文献
76.
Massa KL Murphy CJ Hartmann FA Miller PE Korsower CS Young KM 《Journal of the American Veterinary Medical Association》1999,215(11):1671-1674
OBJECTIVE: To determine the diagnostic value of aerobic microbial culture and cytologic evaluation of corneal specimens in the diagnosis of infectious ulcerative keratitis (IUK). DESIGN: Prospective study. ANIMALS: 48 animals (26 dogs, 13 horses, 7 cats, 1 bird, and 1 llama) with corneal ulcers. PROCEDURE: Scrapings from corneal ulcers were examined cytologically. Corneal swab specimens were submitted for microbial culture. Animals were grouped according to whether they had been receiving antimicrobials at the time of admission. RESULTS: Of the 38 animals receiving antimicrobials, 19 had positive results for IUK on cytologic evaluation, 20 on microbial culture, and 26 on cytologic evaluation, microbial culture, or both. Of the 10 animals not receiving antimicrobials at the time of admission, 7 had positive results for IUK on cytologic evaluation, and 9 had positive results on microbial culture. In this group of 10 animals, additional animals with IUK were not identified on the basis of cytologic evaluation alone. When all 48 animals were considered irrespective of antimicrobial treatment, 26 and 29 had positive results for IUK on cytologic evaluation and microbial culture, respectively, whereas IUK was confirmed in 35 animals on the basis of cytologic evaluation, microbial culture results, or both. CONCLUSIONS AND CLINICAL RELEVANCE: Microbial culture and cytologic evaluation of corneal specimens maximizes identification of IUK, especially in animals receiving antimicrobial treatment. Because of serious consequences of untreated IUK, we recommend that both diagnostic tests be used to tailor treatment and reduce risk of vision impairment in animals. 相似文献
77.
78.
Hathaway MR Dayton WR White ME Henderson TL Young DA Doan TN 《Journal of animal science》1999,77(12):3208-3214
This study was conducted to determine whether an antimicrobially induced (ASP-250) increase in serum IGF-I was the result of differences in feed intake. Serum IGF-I concentrations were measured in crossbred pigs that were fed a control diet or a diet supplemented with ASP-250 either for ad libitum consumption or limited to 85% of the control pigs' consumption. The pigs that consumed either diet ad libitum, control or ASP-250, consumed similar quantities of feed. The ASP-250 ad libitum-intake pigs had serum IGF-I concentrations that were greater (P<.01) than those of their ad libitum-intake control littermates. Similarly, the ASP-250 limit-fed pigs had serum IGF-I concentrations that were greater (P<.01) than those of the controls. Although the serum IGF-I concentrations of pigs fed the ASP-250-supplemented diet for ad libitum intake were greater than the serum IGF-I concentrations of the pigs limit-fed the ASP-250-supplemented diet, the differences were not significant (P<.08). The ASP-250-fed pigs had higher serum IGF binding protein (BP)-3 concentrations than did their control littermates (P<.003). A time course of antimicrobially induced alterations in serum IGF-I concentrations revealed that the effect of increased serum IGF-I levels in ASP-250-supplemented pigs (P<.02) was observed within 4 d and was maintained throughout the 4-wk study. These findings show that feed intake is not responsible for the increase in serum IGF-I observed with ASP-250 supplementation. Additionally, the antimicrobially induced increase in serum IGF-I concentrations occurs within a few days after initiation of the treatment. 相似文献
79.
S Chema S Waghela A D James T T Dolan A S Young W N Masiga A D Irvin G H Mulela L S Wekesa 《The Veterinary record》1986,118(21):588-589
80.