转基因奶牛胚胎移植技术的试验研究

2006年6月至2010年3月以来,北京奶牛中心与中国农业大学合作,在北京奶牛中心良种场开展了转基因牛胚胎移植试验研究。本试验利用中国农业大学培育的转基因母牛作为供体,进行超数排卵生产体内胚胎,将获得的胚胎移植给15～15.5月龄荷斯坦母牛受体。截止2010年3月21日,累计移植转基因奶牛胚胎87枚,移植受体牛87头,妊娠48头,移植妊娠率为55.17%;产犊43头,成活34头,犊牛成活率为79%;经中国农业大学研究人员检测,有14头公牛携带转入目的基因,这14头公牛已全部进入北京市种公牛站饲养。目前,有10头公牛到了试采精月龄,精液经农业部牛冷冻精液质量监督检验测试中心(北京)检测,质量符合国家标准。     

水稻不育系先红A紫叶性状的遗传行为及其应用前景

用新育成的带紫叶色标记的水稻不育系先红A(B)为材料,分别与不同类型的绿叶籼稻、粳稻品种杂交,研究其紫叶性状的遗传行为。结果表明,先红A(B)的紫叶性状由1对紫叶基因控制,一般正常绿叶水稻品种带有1～2对抑制基因可调控该紫叶基因的表达。先红A(B)的紫叶性状及其杂种F1绿叶性状表达稳定,不受光温环境及栽培条件的影响。先红A的紫叶性状是杂交稻种子鉴纯和制种防杂保纯的一种理想标记。先红A配制杂种,可筛选出株叶型好且优势强的组合,显示出良好的应用前景。     

鸽疱疹病毒PCR检测方法的建立及试剂盒的研制

鸽疱疹病毒(PiHV1)感染是由I型鸽疱疹病毒引起的以幼鸽为主的一种常见病毒性传染病,临床上以鼻炎、结膜炎为主要特征。偶尔也会出现精神沉郁、食欲下降、腹泻、呕吐、神经症状。根据已发表的鸽疱疹病毒的基因序列,设计并合成了一对能特异性扩增鸽疱疹病毒的引物,通过对PCR的退火条件的优化,建立了鸽疱疹病毒的PCR检测方法,对发病鸽子进行诊断。并研制出检测试剂盒,再进一步对该试剂盒的敏感性、特异性和稳定性进行了研究,证明该试剂盒具有很高的敏感性和特异性,并且在-20℃条件下可保存1年以上。     

基于非线性结构的铜期货与现货价格关系的实证检验

在期货价格与现货价格间存在非线性结构的条件下，由于传统的线性格兰氏因果关系检验对其非线性因果关系甄别能力弱，因而无法准确判断它们之间的真实关系。本文以上海期货交易所合约铜与现货铜为研究时象，采用修正的Baek-Brock检验方法对我国铜期货价格和现货价格之间的非线性因果关系进行实证研究。实证结果表明铜期货价格和现货价格之间存在双向的非线性因果关系，两者之间并不存在绝对的领先与滞后关系。鉴于价格序列之间的非线性关系可能由于信息流引起，因此，采用EGARCH模型分别对铜期货价格与现货价格序列进行波动率过滤，结果发现经过调整后的价格序列之间仍存在双向的非线性因果关系。     

鸡山农场天然橡胶新品种抗性试验

高产优质抗病甘薯新品种湛薯407是广东省湛江市农业科学研究所以龙薯1号为母本、鸡母薯为父本，通过品种间杂交、多年多点综合鉴定选育而成。2008～2009年参加广东省甘薯新品种区域试验，2010年进行大田生产试验，各试验综合性状表现较好。该品种于2011年5月通过广东省农作物新品种鉴定，鉴定编号为粤审薯2011004。对湛薯407的品质性状、抗病性、产量等进行研究，结果表明：湛薯407薯干品质和食味较好，中抗薯瘟病，鲜薯和薯干产量与广薯111相比增产极显著，适应性广，适宜广东省各薯区种植。根据该品种的生长特性，还提供配套的栽培技术。     

综合利用资源优势开发建设果林大观园--长泰县南亚热带果林大观园的构想与对策

1　建设南亚热果林大观园的优势1.1　南亚热带气候优势长泰属南亚热带海洋性季风气候区 ,年均气温 2 1.7℃ ,平均日照差 8.6℃ ,年均积温 74 2 2 .7℃ ,无霜期 32 8天 ,年均相对湿度 78% ,年均降雨 14 6 2 mm,水热资源丰富 ,冬无严寒 ,夏无酷暑 ,终年温暖湿润。1.2　名优水果优势长泰芦柑在全国柑桔质量评比中 ,连续夺得“三连冠”,又是唯一获得绿色食品使用标志的柑桔 ,建成我国第一个晚芦基地 ,岩溪被誉为“中国芦柑之乡”,枋洋、坂里大力发展中国名柚——文旦柚 ,以水果为主的经济林达 18万亩 ,实现人均一亩园 ,年产千斤果 ,获得福建省水…     

酶联免疫吸附测定(ELISA)检测苏云金杆菌伴孢晶体蛋白质研究

用酶联免疫吸附测定(ELISA)检测Bt伴孢晶体毒素蛋白质,最低可检值7.79ng/0.15ml,灵敏、精确,具高度专化性。测定标准品HI—1—S—1980和Bt乳剂产品中伴孢晶体蛋白质含量,分别为108.06mg/g粉剂和4.57—6.17mg/ml乳剂。用标准品与待测样品(Bt乳剂)的晶体蛋白质含量,就可计算出待测样品的相对毒力效价。ELISA与昆虫生测之间有良好的相关性。     

表达猪链球菌溶血素基因的减毒沙门氏菌的构建及鉴定

将猪链球菌溶血素（suilysin，SLY）基因克隆入原核表达栽体pBV220，将重组质粒再导入减毒鼠伤寒沙门氏菌SV4089株，经PCR和酶切鉴定，构建成携带猪链球菌溶血素基因的重组减毒鼠伤寒沙门氏菌。结果表明：该减毒株具有相对安全性；用酶切和PCR鉴定法证实在无抗生素存在的条件下携带重组质粒的减毒株比较稳定；SDS-PAGE显示SLY能在宿主菌中进行表达。该结果为进一步研究制备猪链球菌口服活疫苗奠定了基础。     

Cytocompatibiltiy of degradable calcium metaphosphate with human marrow mesenchymal stem cells

AIM: To screen the cytotoxicity of degradable calcium metaphosphate (dCMP) compared with hydroxyapatite (HA). The proliferation and differentiation abilities of human marrow mesenchymal stem cells (MSC) were used to exhibit the cytotoxicity. METHODS: The cell morphology of MSC was analysed after direct contact with dCMP at different time points by scanning electron microscopy analysis. The degradation products of dCMP and HA were analysed with inductively coupled plasma torch and ion chromatography. The cytotoxic effect of degradation products of dCMP was evaluated by FACS, quantitative assay of ALP and ARS, respectively. RESULTS: dCMP enhanced the proliferation of MSC, but didn't interfere the osteogenic differentiation process of MSC and its mineralization. HA inhibited the proliferation of MSC and the mineralization of osteogenic differentiated MSC, while it did not interfere the osteogenic differentiation process of MSC. CONCLUSION: dCMP had a better cytocompatibility with MSC than HA, which might allow for its use as skeleton scaffolds.     

玉米雄性不育突变体mi-ms-3的遗传分析及分子鉴定

Maize is one of the best crops in the utilization of heterosis. Male sterile lines are important germplasms for the hybrids production. A male sterile mutant named mi-ms-3 was obtained by screening in a mutator insertion library. The number of male anthers in tassel decreased and not exserted. There were few anthers with only two pollen sacs in the mutant tassels, and some of the anthers were degenerated to membranous and formed filaments at their ends. Although pollens in the anthers could be stained by I₂-KI, pollen shedding was abnormal and the number of pollen grains decreased. The number of silks in the ear of the mutant increased, and there was a sterile grain on both sides of the maturated kernel. Fertility of F₁ plants, which were obtained by hybridization between mi-ms-3 and maize inbred Mo17, was normal. Genetic analysis of F₂ population showed that the mutant phenotype was controlled by a recessive gene. The candidate gene was preliminarily mapped on the long arm of chromosome 3 by BSA and it was located between a SSR marker and an Indel marker with a distance of 1.5 cM. There are 21 candidate genes in this region. It was finally found that the insertion mutation of Mu transposon occurred at 30 bp upstream of the coding region of zm00001d042618 (zmm16) by transponson tagging and sequencing analysis. The results showed that mi-ms-3 was a new allele of sts1, which caused by a single base mutation in the coding region. RT-PCR analysis indicated that the expression of zmm16 in the mutant was decreased. The identification of the new allelic mutant of sts1 in this study would provide new materials for the study of flower development and hybrid seed production.