Infectious pancreatic necrosis virus in Atlantic salmon, Salmo salar L., post-smolts in the Shetland Isles, Scotland: virus identification, histopathology, immunohistochemistry and genetic comparison with Scottish mainland isolates

During mid-June 1999 peak mortalities of 11% of the total stock per week were seen at a sea cage site of Atlantic salmon, Salmo salar L., post-smolts in the Shetland Isles, Scotland. Virus was isolated on chinook salmon embryo (CHSE) cells in a standard diagnostic test and infectious pancreatic necrosis virus (IPNV) identified by enzyme-linked immunosorbent assay. IPNV was confirmed as serogroup A by a cell immunofluorescent antibody test using the cross-reactive monoclonal antibody AS-1. Four weeks after the main outbreak, virus titres in surviving moribund fish were assayed at >10(10) TCID50 g(-1) kidney. Histopathology of moribund fish was characterized by pancreatic acinar cell necrosis and a marked catarrhal enteritis of the intestinal mucosa. In the liver, necrosis, leucocytic infiltration and a generalized cell vacuolation were noted. IPNV-specific immunostaining was demonstrated in pancreas, liver, heart, gill and kidney tissue. The nucleotide sequence of the coding region of segment A was determined from the Shetland isolate. A 1180 bp fragment of the VP2 gene of this isolate was compared with a 1979 reference isolate from mainland Scottish Atlantic salmon, La/79 and another more recent mainland isolate, 432/00. Both A2 isolates were derived from carrier fish without signs of IPN and serotyped by a plaque neutralization test. The Shetland isolate shows a different nucleotide and amino acid sequence compared with the two isolates from carrier fish. These latter isolates showed identical amino acid sequences in the fragment examined, despite the 21 years separating the isolations. Sequence comparisons with other A2 (Sp) isolates on the database confirm all three Scottish isolates are A2 (Sp).     

Evaluation of factors associated with positive IgM capture ELISA results in equids with clinical signs compatible with West Nile virus infection: 1,017 cases (2003)

OBJECTIVE: To describe the prevalence of West Nile virus (WNV) infection and evaluate factors associated with positive IgM capture ELISA results in equids with clinical signs compatible with WNV infection. DESIGN: Retrospective case series. SAMPLE POPULATION: Laboratory submission forms from 1,104 equids tested for WNV in Colorado in 2003. PROCEDURES: Submission forms accompanying samples submitted for detection of WNV via IgM capture ELISA were obtained from the Colorado state veterinarian and diagnostic laboratories performing the tests. Data on signalment, clinical signs, history of vaccination against WNV, and assay results were collected from laboratory submission forms. Equids with clinical signs compatible with WNV infection in which IgM capture ELISA results were positive were considered as case equids. RESULTS: 1,104 equids were tested for WNV; 1,017 (92.1%) had clinical signs compatible with WNV infection. Among equids with clinical signs compatible with WNV infection, the odds of testing positive for WNV via IgM capture ELISA were lower in males and in vaccinated equids and higher in equids with moderate and severe illness, compared with females, unvaccinated equids, and equids with mild illness. CONCLUSIONS AND CLINICAL RELEVANCE: Among equids with clinical signs compatible with WNV infection, vaccination against WNV, severity of clinical signs, duration of illness, and region in Colorado were associated with increased risk of having a positive IgM capture ELISA result.     

生物结皮对高寒退化草地植物群落的影响

生物结皮在各类生态系统中扮演着重要的角色,具有调控土壤水分,养分含量与侵蚀敏感性等作用,并可直接或间接地影响地上植物群落,但对植物群落发展存在促进还是抑制作用尚不清楚。本研究于2017年在青海贵南县森多镇高寒草原,设置大样方,对生物结皮种类和分布格局进行调查。结果表明：在青藏高原高寒退化草地生态系统中,生物结皮的存在显著影响了植物的群落结构,降低了杂草的比例;苔藓生物结皮的盖度与草本植物盖度在初级发育阶段的生物结皮（地衣和苔藓共存）的样方中呈显著正相关关系（P<0.05）,在高级发育阶段的生物结皮（仅有苔藓结皮存在）的样方中正相关关系极显著（P<0.01）。由此可见,生物结皮是高寒草地生态系统的重要组成部分,有调节高寒退化植物群落的结构和功能的作用。     

日粮中添加油料籽实对奶牛生产性能及乳脂脂肪酸组成的影响

本文旨在研究在日粮中添加油料籽实对中国荷斯坦奶牛乳脂肪酸组成及生产性能的影响,为提高奶牛乳脂中共轭哑油酸(CLA)含量,改善乳品质寻找合适的途径.试验选取16头泌乳前期(62±11)d、胎次一致(3胎)、泌乳量相近的中国荷斯坦奶牛,随机分为4组(对照组、菜籽组、亚麻籽组、葵花籽组),每组4头.试验共进行40 d(预试期10 d,正试期30 d),分别在正试期第1天、第15天和第30天测定乳产量、分析乳成分,用气相色谱仪分析乳脂肪酸组成.结果表明,与对照组相比,日粮中添加油料籽实,显著提高了奶牛的泌乳性能(P<0.05),降低了乳脂率(P<0.05),对乳中其他常规成分影响差异不显著(P>0.05);日粮中添加葵花籽后乳脂中CLA含量提高98.28%(P<0.05).菜籽组、亚麻籽组、葵花籽组的乳脂中C14:0和C16:0的含量分别比对照组降低了17.86%和26.75%、15.99%和27.18%、21.99%和21.74%.乳脂中多不饱和脂肪酸(PUFA)含量分别比对照组提高40.83%、47.06%和41.52%(P<0.05).乳脂中的t11-C18:1(x)与CLA(y)的比例(%)存在正相关性,y=0.34x 0.20,R<'=0.696 2(P<0.01).日粮中添加植物油籽显著提高了乳脂中CLA、PUFA的比例,降低了C14:0和C16:0的比例,改善了乳脂的构成,提高了乳品质.     

甘南高寒地区施用有机肥对燕麦草产量和土壤肥力的影响

为探究不同有机肥对高寒地区燕麦草产量及土壤肥力的影响,本试验共设置 ５ 个不同的施肥处理,生育期 120 d后测定了燕麦鲜草产量和干草产量,并分析了土壤的理化性质。试验结果表明：不同的有机肥对高寒地区燕麦草产量影响较大,燕麦鲜草产量结合播种施 1 号燕麦专用有机肥作基肥,在分蘖期追施 2 号燕麦专用有机肥处理组最高,为 82 000 kg/hm2,本试验生产的有机肥处理组次之。此外,经土壤化学性质检测,各处理组有机质含量均较施肥前有所增加,本试验生产的有机肥处理组有机质、全氮、全磷、全钾含量最高,说明以牛羊粪为原料通过益生菌发酵自制的有机肥可以有效提高土壤肥力水平。