Q型烟粉虱芳香基硫酸酯酶B基因的克隆及表达分析

为明确烟粉虱Bemisia tabaci取食感染番茄黄化曲叶病毒(Tomato yellow leaf curl virus,TYLCV)的番茄植株后,其体内的芳香基硫酸酯酶B基因(arylsulfatase B,ARSB)是否能够做出应答反应,基于Q型烟粉虱基因组数据克隆得到ARSB基因cDNA全长,采用生物信息学方法分析其序列特征,并通过实时荧光定量PCR技术测定ARSB基因在Q型烟粉虱不同发育阶段、不同组织及携带TYLCV前后的表达量变化情况。结果显示:Q型烟粉虱ARSB基因的cDNA全长为1 731 bp,编码576个氨基酸,分子量为64.89 kD,具有ARSB的保守结构域。ARSB基因在Q型烟粉虱不同发育阶段均有表达,在卵期表达量最高,成虫期表达量最低;该基因在Q型烟粉虱头胸部的表达量显著高于腹部;Q型烟粉虱获取TYLCV 72 h后其体内ARSB基因表达量显著提高。表明ARSB基因在Q型烟粉虱不同龄期、不同组织内存在差异表达,并可能参与Q型烟粉虱对TYLCV的响应和传毒过程。     

基于无人机技术黄河沿岸沙丘移动速度监测及影响因素分析

为更便捷地监测乌兰布和沙漠黄河沿岸沙丘移动速度并解析其影响因素,该研究以乌兰布和沙漠沿黄段沙丘为研究对象,应用无人机航拍技术开展沿岸沙丘的季节性地貌过程和影响因素研究。结果表明:1)研究区沙丘年移动速率1.08～2.27 m/a,多年平均输沙势为78.82 VU,年合成输沙势为25.92 VU,处于低风能环境,8～12 m/s等级风输沙势是年输沙势的主要部分,约占73.24%。方向变率(合成输沙势(Resultant Drift Potential,RDP)与输沙势(Drift Potential,DP)的比值)RDP/DP保持在0.30～0.46之间,属于中等变率。合成输沙方向RDD为57.83°～107.39°,与沙丘移动方向较为一致,西风组占全年输沙势的52.09%,是沙丘年移动的主要驱动力。2)沙丘移动速率具有明显的季节特征,整体呈现春季移动速率快,冬末-春初次之,秋季与秋末-冬末相近,夏季移动速率最慢。其中,秋末-冬末、春季和秋季输沙势DP 8.48～20.49 VU,合成输沙势方向在90.02°～95.54°之间,RDP/DP值均在0.3～0.8之间,属于中等变率,西风组作用显著,这与年合成输沙方向及沙丘走向较为一致;冬末-春初和春末-夏季分别受东北风(NE)和南风组(SSE、S、SSW)作用,沙丘通过形态变化适应风向,移动速度降缓。季节输沙势主要集中在8～10m/s风速等级,约占整个季节输沙势的40.76%～56.93%。3)综合各季节和年际输沙势与沙丘移动距离呈线性正相关,拟合方程为y=1.02+0.006 62x(R~2=0.339,F=5.616,P=0.045),方程总体显著,输沙势可以表征该地区沙丘移动距离。基于无人机监测的沙丘运动研究综合显示,风况是该地区影响该地区沙丘移动的主要动力,其中西风组8 m/s以上风速是研究区沙丘移动的主要驱动力。风向变率和合成输沙势方向与沙丘移动方向一致时沙丘移动则快,不一致时则缓;无人机可在较大尺度上为沙丘移动提供更为便捷的监测服务,研究结果可为同类地区沙丘移动的无人机监测提供参考依据。     

咸蛋黄快速腌制过程中理化性质变化规律

该研究以新鲜蛋黄为原料,利用快速腌制模具,探究在咸蛋黄的上表面添加食盐单侧腌制过程中,食盐添加量和腌制时间对咸鸡蛋黄快速腌制过程中形貌特征和理化性质变化规律的影响。借助多种仪器分析手段对蛋黄腌制过程中形貌与物性的变化、水分及盐分的迁移规律进行了表征。低场核磁及成像结果表明：在腌制过程中,蛋黄中的水分不断向外迁移,含水率显著降低,当增加食盐的添加量和延长腌制时间,会加快水分的迁移速率;原子吸收结果表明：增加食盐添加盐量越多和腌制时间越长盐分迁移速率越快,质构、色差结果共同表明咸蛋黄腌制过程中,由于水分的向外迁移和盐分的向内渗入,使得蛋黄的蛋白质发生聚集使颜色加深;同时与市售整个腌制后分离的鸡蛋黄产品相比,当腌制时间为7d,添加盐量为3%;腌制时间为3d,添加盐量为5%时,所得的样品与市面的成品咸鸡蛋黄的感官品质及量化指标差异不显著（P<0.05）,为咸蛋黄单侧腌制技术提供理论的可行性。     

新时期农业科研院所学科领域调整和布局的思考——以广东省农业科学院为例

学科建设作为一项综合性、系统性、长期性的基础建设工程,是科研院所的立院之本.文章通过简要分析新时期学科调整的背景和意义,分析了广东省农业科学院在学科建设方面取得的成绩以及存在的不足和短板,在此基础上,提出了加强顶层设计、坚持协同创新、强化条件建设、培育一流学科团队等促进学科建设的对策建议,以期为农业科研院所加强学科建设...     

苗圃化学除草试验研究

<正>化学除草用得好,可以节约大量人力物力,保证苗木健壮成长。以往的试验多局限于木本苗木地中,而在草本花卉地进行除草则很少有人涉足;另外,利用灭生性除草剂进行定向喷雾极易造成药害,不易大面积使用。因此寻找选择性良好的除草剂和探索适宜的用药时机,确定除草剂用药范围,是十分必要的。     

稻米籽粒应力松弛特性的研究

稻米籽粒的松弛特性在其生产、质量控制中起着重要作用。本文通过试验获得了稻米籽粒应力松弛特性的力学指标如Maxwell单元个数、松弛模量、松弛时间等,并研究了稻米籽粒应力松弛特性与稻米品质性状的关系,通过多元逐步回归建立了回归方程,可以为稻米加工装置的设计以及稻米的质量评价提供理论依据,为预测机械损伤、提高产品质量、探索新的控制方法和判断依据提供最佳参数。     

胶州湾北部沿岸浮游植物生态特征的研究

根据1998年4月中旬～10月中旬胶州湾北部的调查结果,共检出浮游植物26属56种,其中硅藻23属50种,甲藻3属6种。浮游植物数量的峰值出现在4月中旬和9月下旬,其均值分别为15115.5×103cell／m3和133315．1×103cell／m3,最低值出现在6～7月。加氏星杆藻、中肋骨条藻和浮动弯角藻曾在该海区形成优势种。     

Effects of dietary protein to energy ratios on growth,body composition and digestive enzyme activities in Chinese mitten‐handed crab,Eriocheir sinensis

This study aimed to determine the optimal protein to energy ratio (P/E ratio) and evaluate the effect of dietary protein and lipid levels on growth performance, body composition and digestive enzymes activities in Chinese mitten‐handed crab, Eriocheir sinensis. Nine practical diets containing three levels both for protein (DP 30%, 35% and 40%) and lipid (DL 2%, 7% and 12%) with P/E ratios ranging from 13.69 to 19.79 mg KJ^?1 were fed to four replicates of crabs (3.39 ± 0.10 g) for 10 weeks. Weight gain increased significantly with the increase in DP level at each DL level. Moreover, weight gain increased in crabs fed with diets containing DL level from 2% to 12% and DP level from 30% to 35%. However, the diet containing 40% DP and 12% DL levels significantly decreased the growth performance and protein efficiency of the crabs. The whole crab and hepatopancreas lipid contents also increased as dietary lipid increased, but not dietary protein. The total protease activity increased significantly with the increase in dietary protein at each lipid level. The lipase activity was statistically comparable among different DL levels at each DP level. Taken together, the crab fed the diet containing 35% protein and 12% lipid levels with P/E 15.77 mg KJ^?1 revealed optimal growth, feed utilization efficiency and digestive enzymes activities. Moreover, our study indicated that the higher dietary lipid level at a relatively lower dietary protein level could provide protein sparing effect in Eriocheir sinensis.     

Survival and serum biochemical responses of spotted sea bass Lateolabrax maculatus during simulated waterless live transportation

Waterless live fish transportation is an alternative and promising transport strategy, which may reduce shipping costs, increase stocking density and improve survival of some species. Spotted sea bass is one of the most economically important marine fish in China. However, waterless transportation has not yet been studied on spotted sea bass. To explore the possibility of waterless transportation of spotted sea bass and its sublethal stress responses, the survival rate and dynamics of blood biochemical parameters were evaluated in the present study by preserving it under no water, less water and water (control) conditions for 9 hr. The serum biochemical parameters including total protein, cortisol, glucose, lactate, alanine aminotransferase (ALT), aspartate aminotransferase (AST), total carbon dioxide (TCO₂), creatinine, blood urea nitrogen (BUN), ammonia, superoxide dismutase (SOD) activity and malonaldehyde (MDA) were investigated. Results showed that the survival rates of fish in all groups gradually declined with time increasing. At the end of transportation, the survival rates in the control group, less‐water group and no‐water group decreased to 49.11%, 15.60% and 23.96% respectively. Significant changes were observed in several selected serum biochemical parameters, including glucose, lactate, ALT, TCO₂, BUN and ammonia during simulated waterless transportation, which might negatively affect the physiological homeostasis of fish, leading to the high mortality in present study. In general, waterless transportation of spotted sea bass was possible, but it might be rather suitable for short time than long time.     

对虾养殖中新生残饵耗氧动态及其规律的研究

本文根据实验模拟研究的结果，估算了由配合饵料和贻贝肉形成残饵后３６天时间内的耗氧量，并讨论了其耗氧动态和规律。残饵耗氧量的变化范围为４．０９～１７．６１ｇ／ｋｇ·ｄ。残饵耗氧曲线随残饵形成时间的不同而显著变化，且始终呈二次曲线衰减形式，在其演变过程中，该二次曲线方程的二次项系数由负数逐渐演变为正数，并最终接近于污染虾塘底质的耗氧曲线。配合饵料和贻贝肉的耗氧曲线变化形式相近，但程度上存在较大差异。另外，本文还初步探讨了残饵的耗氧高峰期，估算了残饵在虾塘底质总耗氧量中的作用。