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101.
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Hoskins AA Friedman LJ Gallagher SS Crawford DJ Anderson EG Wombacher R Ramirez N Cornish VW Gelles J Moore MJ 《Science (New York, N.Y.)》2011,331(6022):1289-1295
The spliceosome is the complex macromolecular machine responsible for removing introns from precursors to messenger RNAs (pre-mRNAs). We combined yeast genetic engineering, chemical biology, and multiwavelength fluorescence microscopy to follow assembly of single spliceosomes in real time in whole-cell extracts. We find that individual spliceosomal subcomplexes associate with pre-mRNA sequentially via an ordered pathway to yield functional spliceosomes and that association of every subcomplex is reversible. Further, early subcomplex binding events do not fully commit a pre-mRNA to splicing; rather, commitment increases as assembly proceeds. These findings have important implications for the regulation of alternative splicing. This experimental strategy should prove widely useful for mechanistic analysis of other macromolecular machines in environments approaching the complexity of living cells. 相似文献
104.
Crawford MK Farneth WE McCarronn EM Harlow RL Moudden AH 《Science (New York, N.Y.)》1990,250(4986):1390-1394
The oxygen isotope effect on the superconducting transition temperature (alpha(o)) varies as a function of x in La2-xSrxCuO(4) and La2-xBaxCuO(4), with the maximum alpha(o) values (alpha(o) >/= 0.5) found for x near 0.12. This unusual x dependence implies that the isotope effect is influenced by proximity to the Abma --> P4(2)/ncm structural phase transition in these systems. Synchrotron x-ray difaction measurements reveal little change in lattice parameters or orthorhombicity due to isotope exchange in strontium-doped materials where alpha(o) > 0.5, eliminating static structural distortion as a cause of the large isotope effects. The anomalous behavior of alpha(o) in both strontium- and barium-doped materials, in combination with the previously discovered Abma --> P4(2)/ncm structural phase-transition in La(1.88)B(0.12)CuO(4), suggests that an electronic contribution to the lattice instability is present and maximizes at approximately 1/8 hole per copper atom. These observations indicate a dose connection between hole doping of the Cu-O sheets, tilting instabilities of the CuO(6) octahedra, and superconductivity in La(2)CuO(4)-based superconductors. 相似文献
105.
Crawford AH Halfacree ZJ Lee KC Brockman DJ 《Journal of Feline Medicine and Surgery》2011,13(10):762-767
Pneumonectomy is the resection of all lung lobes from one side of the thorax. The clinical findings, treatment and outcome of four cases of feline chronic pyothorax managed with exploratory thoracotomy and pneumonectomy are reported. All cases were initially medically managed with thoracic drain placement and antibiosis. However, resolution was not achieved with medical therapy and diagnostic imaging findings consistent with an area of abscessation or marked lung lobe consolidation were identified, supporting a decision for surgical management. Surgical exploration was performed via median sternotomy and, on the basis of gross inspection, non-functional lung was removed. A left-sided pneumonectomy was performed in three cats and a right-sided pneumonectomy in one. All cases survived to discharge and an excellent quality of life was reported on long-term follow-up. Pneumonectomy appears to be well tolerated in the cat. 相似文献
106.
F. Binet L. Fayolle M. Pussard J. J. Crawford S. J. Traina O. H. Tuovinen 《Biology and Fertility of Soils》1998,27(1):79-84
The stimulatory effect of earthworms (Lumbricus terrestris L.) on soil microbial activity was studied under microcosm-controlled conditions. The hypothesis was tested that microbial
stimulation observed in the presence of a soil invertebrate would be due to the utilization of additional nutritive substances
(secretion and excretion products) that it provides. Changes in microbial activity were monitored by measuring simultaneously
CO2 release and protozoan population density. The increase in CO2 released in the presence of earthworms was found to result from both earthworm respiration and enhanced microbial respiration.
The stimulation of microbial activity was confirmed by a significant increase in protozoan population density, which was 3–19
times greater in the presence of earthworms. The respiratory rate of L. terrestris was estimated to be 53 μl O2 g–1 h–1. Earthworm respiration significantly correlated with individual earthworm weight, but there was no correlation between the
increase in microbial respiration and earthworm weight. This finding does not support the hypothesis given above that enhanced
microbial respiration is due to utilization of earthworm excreta. A new hypothesis that relationships between microbial activity
and earthworms are not based on trophic links alone but also on catalytic mechanisms is proposed and discussed.
Received: 26 August 1997 相似文献
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R A Dietrich S H Amosson R P Crawford V C Beal 《Journal of the American Veterinary Medical Association》1991,198(1):78-80
Initial and cumulative reactor rates for strain 19 vaccinates and nonvaccinates were significantly (P less than 0.001) lower for beef herds containing variable proportions of vaccinates, compared with reactor rates in nonvaccinated herds. In addition, significant (P less than 0.005) reduction in cumulative incidence was observed in nonvaccinated and strain 19-vaccinated cattle as the proportion of vaccinates within the herd increased from 1 to 19%, 20 to 39%, 40 to 59%, and 60 to 100%. Duration of quarantine and number of herd tests were not reduced in herds with strain 19-vaccinated cattle. In herds released from quarantine, duration of quarantine and number of tests were positively correlated to proportion of the herd vaccinated. In nonvaccinated herds released from quarantine, effect of herd size was documented by strong positive (P = 0.042) correlation with duration of quarantine and slightly weaker correlation (P = 0.095) with number of tests. 相似文献
109.
110.
Guidelines for the future of equine locomotion research 总被引:1,自引:0,他引:1
This paper describes the major areas that require investigation in equine locomotion and outlines a plan for such research in both the Thoroughbred and Standardbred. Fifteen topics for future investigation have been selected and given an order of priority. These topics deal with characteristics of normal locomotion, factors which influence locomotion, the epidemiology and economics of lameness and the clinical identification of lameness. 相似文献