全文获取类型
收费全文 | 367篇 |
免费 | 32篇 |
国内免费 | 1篇 |
专业分类
林业 | 6篇 |
农学 | 11篇 |
基础科学 | 3篇 |
37篇 | |
综合类 | 39篇 |
农作物 | 10篇 |
水产渔业 | 63篇 |
畜牧兽医 | 139篇 |
园艺 | 8篇 |
植物保护 | 84篇 |
出版年
2023年 | 4篇 |
2021年 | 12篇 |
2020年 | 9篇 |
2019年 | 7篇 |
2018年 | 13篇 |
2017年 | 6篇 |
2016年 | 6篇 |
2015年 | 8篇 |
2014年 | 8篇 |
2013年 | 28篇 |
2012年 | 10篇 |
2011年 | 9篇 |
2010年 | 7篇 |
2009年 | 15篇 |
2008年 | 20篇 |
2007年 | 17篇 |
2006年 | 17篇 |
2005年 | 19篇 |
2004年 | 12篇 |
2003年 | 15篇 |
2002年 | 12篇 |
2001年 | 11篇 |
2000年 | 10篇 |
1999年 | 13篇 |
1998年 | 13篇 |
1997年 | 2篇 |
1996年 | 3篇 |
1995年 | 10篇 |
1994年 | 4篇 |
1993年 | 2篇 |
1992年 | 4篇 |
1990年 | 5篇 |
1989年 | 2篇 |
1988年 | 2篇 |
1986年 | 4篇 |
1985年 | 3篇 |
1984年 | 7篇 |
1983年 | 3篇 |
1982年 | 2篇 |
1981年 | 3篇 |
1980年 | 2篇 |
1979年 | 6篇 |
1978年 | 2篇 |
1976年 | 6篇 |
1975年 | 2篇 |
1973年 | 4篇 |
1971年 | 3篇 |
1970年 | 5篇 |
1969年 | 2篇 |
1893年 | 1篇 |
排序方式: 共有400条查询结果,搜索用时 31 毫秒
71.
I.J. Cooke 《The Journal of Horticultural Science and Biotechnology》2013,88(3):285-292
The influence of far-red light on the development of tomato seedlings was investigated. It was found that a deficiency of it in the incident spectrum retarded stem elongation. The quantity of far-red light in the flux did not affect the number of flowers forming on the first truss or the time of anthesis. 相似文献
72.
Popescu F Wyder M Gurtner C Frey J Cooke RA Greenhill AR Posthaus H 《Veterinary microbiology》2011,153(1-2):173-177
Clostridium perfringens type C causes fatal necrotizing enteritis in different mammalian hosts, most commonly in newborn piglets. Human cases are rare, but the disease, also called pigbel, was endemic in the Highlands of Papua New Guinea. Lesions in piglets and humans are very similar and characterized by segmental necro-hemorrhagic enteritis in acute cases and fibrino-necrotizing enteritis in subacute cases. Histologically, deep mucosal necrosis accompanied by vascular thrombosis and necrosis was consistently reported in naturally affected pigs and humans. This suggests common pathogenetic mechanisms. Previous in vitro studies using primary porcine aortic endothelial cells suggested that beta-toxin (CPB) induced endothelial damage contributes to the pathogenesis of C. perfringens type C enteritis in pigs. In the present study we investigated toxic effects of CPB on cultured primary human macro- and microvascular endothelial cells. In vitro, these cells were highly sensitive to CPB and reacted with similar cytopathic and cytotoxic effects as porcine endothelial cells. Our results indicate that porcine and human cell culture based in vitro models represent valuable tools to investigate the pathogenesis of this bacterial disease in animals and humans. 相似文献
73.
Mehmet E. Göre Nedim Altın Kevin Myers David E. L. Cooke William E. Fry Göksel Özer 《Plant pathology》2021,70(4):898-911
Late blight, caused by the oomycete Phytophthora infestans, is considered the most important and destructive disease of potato in Turkey. In this study, characterization of 367 isolates of P. infestans obtained from the potato-growing areas of the country was carried out to evaluate the pathogen population structure over the 2017–2019 production seasons. The isolates were characterized by numerous features including mating type, in vitro mefenoxam sensitivity, simple-sequence repeat (SSR) markers, and virulence against a set of potato differential lines. Most isolates were A2 mating type (353 isolates). Also, 68% of isolates were resistant to mefenoxam; the remainder were intermediate in their sensitivity and there were no sensitive isolates. SSR-based genotypic analysis of P. infestans populations showed a low genetic diversity. The 13_A2 clonal lineage predominated with a frequency of 92.1%, followed by 34_A1 (3.3%) and 37_A2 (2.7%). Genotypes 34_A1 and 37_A2 were detected only in 2019. This is the first report of 34_A1 and 37_A2 clonal lineages causing late blight disease of potato in Turkey. The most abundant virulence type was one overcoming resistance genes R1, R2, R3, R4, R6, R7, R10, and R11. These results emphasized that the migration of individuals and the asexual generation of subclonal differences were the main factors driving the population structure of P. infestans in Turkey. 相似文献
74.
Summary Of 250 isolates ofPhytophthora infestans obtained from Northern Ireland potato crops between 1981 and 1993, only six proved to be of the A2 mating type, the remainder
being A1. The first A2 isolate was obtained from a tuber in 1987. The frequency of A2 isolates from 1987 onwards was 3%, a
lower incidence than has been reported for England and Wales and the Republic of Ireland. There was no association between
phenylamide resistance and mating type. 相似文献
75.
S. Nolan B.M. Cooke 《European journal of plant pathology / European Foundation for Plant Pathology》2000,106(2):203-207
A field study is described which explored the possibility of controlling Stagonospora nodorum and Septoria tritici on wheat using a barley pathogen, Drechslera teres. Pre-treatment of wheat cv. Hussar flag leaves with D. teres resulted in a significant reduction in disease caused by S. nodorum and S. tritici, resulting in a significant increase in grain yield. When cv. Brigadier leaves were treated with D. teres prior to inoculation with S. nodorum there was an initial increase in disease expression whilst D. teres had no effect on symptoms produced by S. tritici on cv. Brigadier. There was significantly less disease on leaves of cvs. Hussar and Brigadier pre-treated with D. teres prior to inoculation with an equal mixture of S. nodorum and S. tritici compared to plants pre-treated with water. It is concluded that D. teres and other non-host pathogens show potential as biological control agents for S. nodorum and S. tritici. 相似文献
76.
S J Cooke K P Chandroo T A Beddow R D Moccia & R S McKinley 《Aquaculture Research》2000,31(6):495-505
Rainbow trout Oncorhynchus mykiss (Walbaum) are usually cultured at high densities to maximize production, but little is known about the physiological and behavioural consequences of high‐density fish culture. The purpose of this study was to develop quantitative correlates of activity for fish held under conditions of increasing density. Fifteen hatchery‐reared rainbow trout (mean fork length = 432.3 ± 9.2 mm) were implanted with activity (electromyogram; EMGi) transmitters and randomly assigned to each of three replicate tanks. Original tank densities (15 kg m?3) were then increased to 30 and finally to 60 kg m?3 at weekly intervals by adding additional fish. Remote telemetry signals indicated that activity increased with increasing stocking density. Fish were relatively inactive during the middle of the day, with diel activity patterns not differing among treatments. Fish were more active during periods of darkness, with activity increasing with increasing stocking density. Relationships between swimming speed, EMGi activity and oxygen consumption were developed using a respirometer and used to estimate oxygen consumption of the fish in the density treatments. Average oxygen consumption estimates increased with increasing density treatments as follows: low density = 75.6 mg kg?1 h?1; medium density = 90.0 mg kg?1 h?1; and high density = 102.6 mg kg?1 h?1. Telemetry permits quantification of the effects of increasing density on fish activity. Physiological telemetry devices may provide a useful tool for remotely monitoring animal welfare correlates under controlled conditions for fish exposed to different husbandry conditions and may prove a valuable tool for the aquaculture industry. 相似文献
77.
Steven J Cooke Zeb S Hogan Paul A Butcher Michael J W Stokesbury Rajeev Raghavan Austin J Gallagher Neil Hammerschlag Andy J Danylchuk 《Fish and Fisheries》2016,17(1):249-265
Recreational angling has been implicated in population declines of some marine and freshwater fish, but this activity is rarely considered as a threat or even halted when endangered species are targeted. Indeed, in some cases, anglers are drawn to fish for rare or endangered species. Conservation‐oriented behaviours such as catch‐and‐release are often practiced voluntarily due to the ethics of anglers, yet even in these cases, some fishing mortality occurs. Nonetheless, there are many indirect conservation benefits associated with recreational angling. Here, we present a series of case‐studies and consider whether catch‐and‐release angling for endangered fish is a conservation problem or a conservation action. If recreational angling activities contribute to population‐level consequences that are contrary to recovery strategies, then angling for endangered species would seem to be a poor option. However, as revealed by several case‐studies, there is much evidence that anglers are vocal and effective proponents of fish and habitat conservation, and for endangered species, they are often the only voice when other stakeholders are not engaged. Anglers may contribute directly to conservation actions via user fees (e.g. licences), philanthropic donations or by volunteering in research, education and restoration activities. However, it is important to quantify post‐release mortality as well as understand the full suite of factors influencing a given population or species to know the potential risks. A risk assessment approach outlined in the paper may be used by managers to determine when the benefits of angling for endangered species outweigh the risks. 相似文献
78.
C.?Silvar J.?M.?Duncan D.?E.?L.?Cooke N.?A.?Williams J.?Díaz F.?MerinoEmail author 《European journal of plant pathology / European Foundation for Plant Pathology》2005,112(1):43-52
A PCR-based method was developed for the identification and detection of Phytophthora capsici in pepper plants. Three PCR primers (CAPFW, CAPRV1 and CAPRV2) specific for P. capsiciwere designed based on the sequence of its internal transcribed spacer regions. CAPFW/CAPRV1 amplify a 452 bp product from P. capsici DNA whereas CAPFW/CAPRV2 a 595 bp fragment; neither set amplifies DNA from pepper or several fungi pathogenic to pepper. In conventional (single-round) PCR, the limit of detection was 5 pg DNA for both primer sets, whereas in nested PCR the detection limit for both was of 0.5 fg. However, when the dilution series of target DNA were spiked with plant DNA, amplification declined two-fold in both conventional and nested PCR. The CAPFW/CAPRV2 set in conventional PCR was used to detect P. capsici DNA in inoculated plants. Detection occurred as soon as 8h post-inoculation in stem samples from infected but still symptomless plants. The method was also tested to detect fungal DNA in infected soils. 相似文献
79.
80.
Storage of plasma at -30 degrees C, with no prior treatment, resulted in significant (P less than 0.01) reductions in the biological and immunological activities of oxytocin after 10 days and further reductions after 20 days. Acidification with 0.1 N hydrochloric acid, or snap-freezing in solid carbon dioxide-acetone, before storage afforded some protection against these losses and a combination of both treatments preserved the biological and immunological activities for 20 days despite thawing and refreezing after 10 days. 相似文献