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排序方式: 共有293条查询结果,搜索用时 368 毫秒
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Amanda M. Berrian Beatriz Martínez‐Lpez Vanessa Quan Patricia A. Conrad Jacques van Rooyen Gregory J. G. Simpson John Frean Jacqueline Weyer Jennifer Rossouw Darryn Knobel Lucille Blumberg 《Zoonoses and public health》2019,66(5):458-469
Endemic zoonoses, such as Q fever and spotted fever group (SFG) rickettsiosis, are prevalent in South Africa, yet often undiagnosed. In this study, we reviewed the demographics and animal exposure history of patients presenting with acute febrile illness to community health clinics in Mpumalanga Province to identify trends and risk factors associated with exposure to Coxiella burnetii, the causative agent of Q fever, and infection by SFG Rickettsia spp. Clinical and serological data and questionnaires elucidating exposure to animals and their products were obtained from 141 acutely febrile patients between 2012 and 2016. Exposure or infection status to C. burnetii and SFG Rickettsia spp. was determined by presence of IgG or IgM antibodies. Logistic regression models were built for risk factor analysis. Clinical presentation of patients infected by SFG rickettsiosis was described. There were 37/139 (27%) patients with a positive C. burnetii serology, indicative of Q fever exposure. Patients who had reported attending cattle inspection facilities (“dip tanks”) were 9.39 times more likely to be exposed to Q fever (95% CI: 2.9–30.4). Exposure risk also increased with age (OR: 1.03, 95% CI: 1.002–1.06). Twenty‐one per cent of febrile patients (24/118) had evidence of acute infection by SFG Rickettsia spp. Similarly, attending cattle inspection facilities was the most significant risk factor (OR: 8.48, 95% CI: 1.58–45.60). Seropositivity of females showed a significant OR of 8.0 when compared to males (95% CI: 1.49–43.0), and consumption of livestock was associated with a decreased risk (OR: 0.02, 95% CI: 0.001–0.54). A trend between domestic cat contact and SFG rickettsiosis was also noted, albeit borderline non‐significant. In this endemic region of South Africa, an understanding of risk factors for zoonotic pathogens, including exposure to domestic animals, can help clinic staff with diagnosis and appropriate therapeutic management of acutely febrile patients as well as identify target areas for education and prevention strategies. 相似文献
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Patterns of photosynthesis and starch allocation in seedlings of four bottomland hardwood tree species subjected to flooding 总被引:5,自引:0,他引:5
Effects of short-term (32 days) flooding on photosynthesis, stomatal conductance, relative growth rate and tissue starch concentrations of flood-intolerant Quercus alba L. (white oak), bottomland Quercus nigra L. (water oak), bottomland Fraxinus pennsylvanica Marshall. (green ash) and flood-tolerant Nyssa aquatica L. (water tupelo) seedlings were studied under controlled conditions. Net photosynthetic rates of flooded N. aquatica seedlings were reduced by 25% throughout the 32-day flooding period. Net photosynthetic rates of flooded Q. alba seedlings fell rapidly to 25% of those of the control seedlings by Day 4 of the flooding treatment and to 5% by Day 16. In F. pennsylvanica and Q. nigra, net photosynthetic rates were reduced to 50% of control values by Day 8 but remained at approximately 30 and 23%, respectively, of control values by Day 32. Leaves of flooded Q. alba seedlings accumulated approximately twice as much starch as leaves of non-flooded control plants, whereas root starch concentrations decreased to 67% of those of control plants by the end of the 32-day flooding treatment. In contrast, flooding caused only a small increase in leaf starch concentrations of N. aquatica plants, but it increased root starch concentrations to 119% of those of the control plants by the end of the experiment. The co-occurring bottomland species, Fraxinus pennsylvanica and Q. nigra, differed from each other in their patterns of stomatal conductance and root starch concentrations. We conclude that the maintenance of low leaf starch concentrations, and high pre-flood root tissue starch concentrations are important characteristics allowing flood-tolerant species to survive in flooded soils. 相似文献
65.
Tay SL Kasapis S Perera CO Barlow PJ 《Journal of agricultural and food chemistry》2006,54(16):6046-6053
The purpose of this investigation is to develop a better understanding of the structure-function relationship of the 2S fraction of soy protein that has not been considered in earnest by the research community. Defatted soy flour was used to extract the three major fractions of the protein (2S, 7S, and 11S). It was found that 2S exhibits better foaming and emulsification properties than the other two molecular fractions. Work was extended to structural properties, which were monitored using spectrophotometry, atomic force microscopy, scanning electron microscopy, small-deformation dynamic oscillation on shear, and large-deformation compression testing. An experimental protocol utilizing glucono-delta-lactone (GDL), GDL with N-ethylmaleimide, or GDL with urea was capable of identifying the nature of molecular interactions responsible for gelation. Surprisingly, it was found that in the initial stages of structure formation, 2S fared better than 7S, with 11S exhibiting the highest rates of aggregation. Given time, however, 7S produced a firmer network with a better water-holding capacity than that of 2S. Non-covalent interactions, as opposed to disulfide bridging, were found to be largely responsible for the changing functionality of the molecular fractions throughout the experimentation from the formation of a vestigial structure to that of a mature gel. 相似文献
66.
Stoddard RA Atwill ER Conrad PA Byrne BA Jang S Lawrence J McCowan B Gulland FM 《Veterinary microbiology》2009,133(3):264-271
The aim of this study was to determine if antimicrobial drug use increases resistance of commensal gastrointensinal Escherichia coli of wild northern elephant seals (Mirounga angustirostris) treated in rehabilitation, and, if so, identify the risk factors involved. Minimum inhibitory concentration (MIC) levels of twelve antimicrobial drugs were determined for 289 E. coli isolates from 99 seals sampled at admission and 277 isolates obtained at release from rehabilitation using broth microdilution. Prevalence of E. coli antimicrobial resistance, MIC(50), MIC(90), and clustering of MIC values were determined for seals and the data were analyzed using Fisher's exact test, ordinal logistic regression and negative binomial regression. At release from rehabilitation 77.8% of the seals had antimicrobial resistant E. coli compared to 38.4% of the seals at admission. The MIC(90) for amoxicillin-clavulanic acid, chloramphenicol, enrofloxacin, ticarcillin-clavulanic acid, and trimethoprim-saulfamethoxazole were at levels considered to be sensitive at admission but they increased to levels of resistance at release. E. coli were grouped into four clusters by their MIC values, with increasing levels of resistance going from Cluster 1 to 4. A primary risk factor associated with the probability of a seal having E. coli in Clusters 3 and 4 was time in rehabilitation, regardless of whether the animal received treatment with antimicrobial drugs, suggesting nosocomial infection. The results of this study provide evidence that increased levels of hygiene and appropriate use of antimicrobial therapy might be important in the rehabilitation of wild animals to prevent rise in the prevalence of antimicrobial resistant bacteria. 相似文献
67.
Freuling CM Kliemt J Schares S Heidecke D Driechciarz R Schatz J Müller T 《Berliner und Münchener tier?rztliche Wochenschrift》2012,125(5-6):255-258
In Europe bat rabies in Daubenton's bats (Myotisdaubentonii) and in Pond bats (Myotis dasycneme) caused by the European bat lyssavirus 2 (EBLV-2) has been confirmed in less than 20 cases to date. Here we report the second encounter of this virus species in Germany. A Daubenton's bat found grounded in the zoological garden in Magdeburg died shortly after. In the frame of a retrospective study the bat carcass was eventually transferred to the national reference laboratory for rabies at the Friedrich-Loeffler-Institute for rabies diagnosis. Lyssavirus was isolated and characterized as EBLV-2. 相似文献
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Macintire DK Boudreaux MK West GD Bourne C Wright JC Conrad PA 《Journal of the American Veterinary Medical Association》2002,220(3):325-329
OBJECTIVE: To identify subclinical Babesia gibsoni infection in American Pit Bull Terriers from the southeastern United States and to determine the genetic sequence of parasite DNA isolated from these dogs. DESIGN: Case series. ANIMALS: 33 American Pit Bull Terriers and 87 dogs of various other breeds. PROCEDURE: Blood smears were examined for microscopic evidence of the parasite, and DNA was extracted from blood samples and used in a polymerase chain reaction (PCR) assay designed to amplify the small subunit ribosomal RNA gene sequence of B. gibsoni. Amplification products of the expected size were sequenced, and sequences were compared with published sequences for B. gibsoni isolates. Hematocrit, platelet count, mean platelet volume, WBC count, and eosinophil count were compared between dogs with positive PCR assay results and dogs with negative results. RESULTS: Results of the PCR assay were positive for 18 of the 33 (55%) American Pit Bull Terriers, including all 10 dogs with microscopic evidence of parasitemia. Only 1 of these dogs was clinically ill at the time blood samples were collected. Results of microscopic evaluation of blood smears and of the PCR assay were negative for the 87 other dogs. Hematocrit and platelet count were significantly lower in dogs with positive PCR assay results than in dogs with negative results. CONCLUSIONS AND CLINICAL RELEVANCE: Results suggest that American Pit Bull Terriers in the southeastern United States may be subclinically infected with B. gibsoni. However, subclinical infection was not identified in dogs of other breeds from the same geographic area. 相似文献