Single layer centrifugation of fresh dromedary camel semen improves sperm quality and in vitro fertilization capacity compared with simple sperm washing

Single layer centrifugation (SLC) through a colloid is a tool for selecting viable mammalian spermatozoa but has not been used previously for fresh dromedary camel sperm. Semen from six camels (2 ejaculates/male) was diluted 1:5 (v:v) or 1:10 (v:v) in a Tris–citrate–fructose buffer for mechanical liquefaction by gentle pipetting. Following liquefaction, semen was processed either by SLC or by centrifugation without a colloid (control). Total and progressive motilities, CASA kinematics, vitality and acrosome integrity (eosin–nigrosin) and plasma membrane integrity (Hypo‐osmotic swelling test; HOST), and fertilizing ability in a heterologous assay (zona‐free goat oocytes) were evaluated. Both total (p = .003) and progressive motilities (p = .003) were higher in SLC‐processed than in control semen samples, irrespective of dilution. Positive HOST values increased when using colloid in 1:5 (p = .001) and 1:10 dilution (p = .010). Colloid‐selected sperm had higher penetration rates than controls (p < .001 and p = .02 for 1:5 and 1:10 dilutions, respectively). However, only the SLC sperm at 1:5 dilution showed higher percentages of pronuclear formation (p = .02) than controls. Dilution effect was only significant for total motility before in vitro fertilization, with higher values for the 1:5 dilution (p = .033). The recovery rates of motile sperm between dilutions were similar (26.1% vs 35.4%; p = .226). In conclusion, SLC is a promising tool for selecting functional dromedary camel sperm and warrants more research.     

Using rainfall simulation to guide planning and management of rehabilitated areas: Part II. Computer simulations using parameters from rainfall simulation

This paper reports computer simulations carried out using data from a rainfall simulator study on a steeply sloping revegetated area (a soundbund) at the NorthParkes Mine, near Parkes in New South Wales, Australia. Simulations of slope stability used a combination of daily time‐step models to consider soil water balances and runoff, growth of grass/legume pasture and soil erosion. Simulations indicated that long‐term erosion rates on the soundbund would be low (1·01–1·27 t ha⁻¹ yr⁻¹) due to low runoff rates and good vegetative cover. The simulations highlighted that peak vegetative growth occurred prior to the period of peak erosion hazard, with carry‐over dry matter being crucial for erosion control during the period of peak rainfall erosivity. This, in turn, indicated that grazing during the periods of peak growth and peak erosion hazard should be avoided (to avoid loss of dry matter). It also indicated that there was value in establishing grass species that would give greater growth during summer months, and that may produce dry matter that is more persistent through time. Simulations of stormwater flows in channels draining various lifts on the soundbund compared a range of alternatives with respect to channel length, gradient and roughness. The simulations indicated little effect of channel length on peak flow rates or depths once channel lengths exceeded 250 m and also showed little effect of vegetative roughness on peak flow rates or depths once Manning's n values exceeded 0·05. Implications of these results for design of drainage channels and drop structures on the soundbund are discussed. Copyright © 2000 John Wiley & Sons, Ltd.