Sequential bacteriological observations in relation to cell-mediated and humoral antibody responses of cattle infected with Mycobacterium paratuberculosis and maintained on normal or high iron intake

Twenty calves were orally infected with Mycobacterium paratuberculosis before weaning. Ten of these plus 4 non-infected controls were maintained on elevated dietary iron intake from 6 to 33 months of age. During this time, in which the majority of animals were bred, the influence of increased dietary iron upon tests of cellular and humoral immune responsiveness to antigens of the organism were monitored. Results were examined in relation to the organism's capacity to multiply and infect up to 7 portions of the intestinal tract. No significant differences were detected in the degree of intestinal disease or pattern of faecal excretion of M. paratuberculosis in iron supplemented and non-supplemented cattle. Cutaneous delayed-type hypersensitivity (DTH) to johnin PPD developed at 1 month and in-vitro lymphocyte and immunostimulatory activity (LS) to this antigen at 2 months after infection. LS indices were significantly reduced in magnitude in iron-supplemented cattle (p less than 0.01). Most ELISA antibody responses were positive 10 to 17 months after infection and preceded the fewer number of CF responses by several months. Neither of the antibody tests was affected by elevated iron intake. Generally, complete or partial resistance to paratuberculosis was associated with sustained positive monthly LS tests (index greater than or equal to 2.0), whereas antibody levels tended to be sustained only in the more severely affected cattle. Although neither test system was affected by pregnancy the ELISA failed to detect a significant proportion of cattle chronically shedding M. paratuberculosis in faeces.     

Effects of IGF‐1 on In Vitro Culture of Bovine Preantral Follicles are Dose‐Dependent

This study aimed at assessing the effect of different concentrations of the growth factor similar to insulin 1 (IGF‐1) in the development, survival and ultrastructure of the bovine preantral follicles cultured in situ. Fragments of bovine ovarian cortical tissue were cultured during 1 and 7 days in 1 ml of α‐MEM⁺, supplemented with different concentrations of human recombinant IGF‐1 (0, 30, 70 and 100 ng/ml), in an incubator at 37°C and 5% of CO₂ in 24‐well plates with total replacement of the medium every 2 days. Non‐cultured ovarian fragments (control) and ovarian fragments cultured during 1 and 7 days were processed for classic histology, mechanical isolation and electron transmission microscopy (ETM). Parameters such as normality, viability, activation, development, diameter and ultrastructure were evaluated. All statistical analyses were carried out using sas Version 9.2. The results showed that the percentage of follicles morphologically normal in the IGF‐1 30 ng/ml treatment was similar to the fresh control (p > 0.05) both on the day 1 and on the day 7 of in vitro culture. In the viability analysis, the cultured treatments maintained the percentage of viable follicles during the entire culture period (p > 0.05). After 7 days of culture, the IGF‐1 30 ng/ml treatment showed higher percentages of developing follicles (48.33%) than those of the fresh control (22.22%) and the cultured treatments (p < 0.05). Also, after 7 days of culture, IGF‐1 30 ng/ml presented a higher follicular diameter when compared to the control and other concentrations of IGF‐1 tested. Ultrastructurally, the non‐cultured control and IGF‐1 30 ng/ml, after 7 days of culture, showed conserved oocytes, nuclei and organelles. Hence, it is concluded that IGF‐1 30 ng/ml was the most efficient concentration for the development of bovine preantral follicles cultured in vitro.     

Nitrogen mineralization in relation to site history and soil properties for a range of Australian forest soils

Rates of N mineralization were measured in 27 forest soils encompassing a wide range of forest types and management treatments in south-east Australia. Undisturbed soil columns were incubated at 20°C for 68 days at near field-capacity water content, and N mineralization was measured in 5-cm depth increments to 30 cm. The soils represented three primary profile forms: gradational, uniform and duplex. They were sampled beneath mature native Eucalyptus sp. forest and from plantations of Pinus radiata of varying age (<1 to 37 years). Several sites had been fertilized, irrigated, or intercropped with lupins. The soils ranged greatly in total soil N concentrations, C:N ratios, total P, and sand, silt, and clay contents. Net N mineralization for individual soil profiles (0–30 cm depth) varied from 2.0 to 66.6 kg ha^-1 over 68 days, with soils from individual depths mineralizing from <0 (immobilization) to 19.3 kg ha^-1 per 5 cm soil depth. Only 0.1–3.1% of the total N present at 0–30 cm in depth was mineralized during the incubation, and both the amount and the percentage of total N mineralized decreased with increasing soil depth. N fertilization, addition of slash residues, or intercropping with lupins in the years prior to sampling increased N mineralization. Several years of irrigation of a sandy soil reduced levels of total N and C, and lowered rates of N mineralization. Considuring all soil depths, the simple linear correlations between soil parameters (C, N, P, C:N, C:P, N:P, coarse sand, fine sand, silt, clay) and N mineralization rates were generally low (r<0.53), but these improved for total N (r=0.82) and organic C (r=0.79) when the soils were grouped into primary profile forms. Prediction of field N-mineralization rates was complicated by the poor correlations between soil properties and N mineralization, and temporal changes in the pools of labile organic-N substrates in the field.     

姬松茸深层发酵生产胞外多糖的碳氮源研究

研究表明摇床振荡培养有利于姬松茸菌丝体生长，而静止培养有利于其胞外多糖的合成。以胞外多糖为目标的研究中。为排除定粉类多糖（碳源）的干扰，红糖是比较理想的碳源，红糖的浓度以20g／L以内，酵母膏是较好的氮源。     

Contested notions of disaster justice during the 2011 Bangkok floods: Unequal risk,unrest and claims to the city

The 2011 Bangkok floods, a slow onset event, flooded significant parts of the city. The state's response to flooding followed a traditional cultural and hierarchical approach to justice within Thailand, stemming from Buddhist values, an informal caste system and monarchical order. This resulted in a spatially uneven outcome, with the ‘preservation’ of the central city and two‐month‐long floods in outer suburbs, exacerbated by inadequate management and coordination. Suburban communities sought a more egalitarian notion of disaster justice, with hazard burdens shared more equitably and people having adequate access to decision‐making over the distribution of disaster risk and compensation for damage. Extensive damage, and reduced livelihoods, caused friction between the two views, and protests, contestations and conflicts during the floods, in four different community contexts in northern Bangkok. By generating alternative discourses, challenging the hierarchical notion of justice and taking direct action to removing floodwalls, protesters sought to reshape the spatiality of the floodwater and claim inclusive citizenship and their right to the city. Existing perceptions of disaster justice, usually focused on liberal state responses to understanding disasters as natural episodes, are alone inadequate to explain the outcomes of and responses to disasters in different cultural contexts.     

Structural requirements for repellency: norsesquiterpenes and sesquiterpenoid derivatives of nootkatone against the Formosan subterranean termite (Isoptera: Rhinotermitidae)

BACKGROUND: Research has shown that the family of grapefruit flavors called nootkatones have significant repellant and toxic effects to Formosan subterranean termites (Coptotermes formosanus Shiraki). RESULTS: Nineteen synthetic nootkatone derivatives, along with three commercially available nootkatone derivatives, were tested for repellent activity against C. formosanus by a choice assay in a petri dish with a two‐step triage procedure. Based on the repellency threshold value, the relationships between structure and activity are discussed. CONCLUSION: Four derivatives of nootkatone have very high repellency and toxicity to C. formosanus, 9 times the potency of the primary compound nootkatone. Four other compounds have between 2 and 3 times the repellency of nootkatones, and three compounds are equal in their repellency to nootkatone. Copyright © 2010 Society of Chemical Industry     

热带四种按树人工林的土壤指标对氮肥反应的评价

较低氮利用性经常会导致桉树人工林的减产.为检验氮矿化的土壤指标的利用并预测桉树对添加氮肥的响应,研究四种桉树人工林(印度海岸低洼地2种细叶桉人工林和和喀拉拉邦高地的2种巨桉人工林)对添加氮肥和相关季节性氮矿化以及其他氮利用率指标的响应,测定几种生化指标对氮肥的响应,包括全氮,土壤C:N比和在厌氧、有氧孵化过程中释放的氮.结果表明,在4块实验地里,增加氮肥可以相应的提高生产率7%至70%;在有氧孵化过程中,释放的氮与氮肥间有最显著相关性(R2=0.92,p<0.01).与有氧孵化过程中释放的氮相比,季节性土壤氮矿化作用是一个较差的肥料响应的指标.虽然有的指标有很好的应用价值,但在广泛应用之前还需验证测试.     

Effects of management techniques on the establishment of eucalypt seedlings on farmland: a review

Research into the effects of seven management techniques on survival and growth of eucalypt seedlings planted on farmland is reviewed. The techniques include: pre- and post-planting weed control; soil cultivation; fertiliser; mulch; tree guards/shelters; and irrigation. The initial and ongoing effects of each technique are discussed—including the effects of timing, type and quantity. Consideration is given to site, species and climatic influences. A statistical analysis of the published survival rates is then presented, to provide information on the relative importance of, and interactions between, practices. The analysis shows that maximum survival may be achieved by using one or two management techniques. Combining this result with the insights gained from the review suggests that the use of soil cultivation and post-planting weed control are likely to achieve the greatest improvements in early eucalypt survival and growth.