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41.
The effect of acidic precipitation on cation leaching in a second-growth Douglas-fir ecosystem at the Thompson Research Center is reviewed. Sulfate mobility and soil pH buffering power were tested by applications of heavy doses of H2SO4 to the study plot. Sulfate at high concentrations proved to be immobilized, presumably by adsorption to soil sesquioxide surfaces. Soil sulfate adsorption was determined at varying sulfate concentrations, and two mechanisms of adsorption are implied by the shapes of the isotherms. 相似文献
42.
43.
Histone structure: asymmetric distribution of lysine residues in lysine-rich histone 总被引:8,自引:0,他引:8
Structural studies on a very lysine-rich histone show that the carboxyl-terminal half of the molecule is enriched in lysine (and proline). which suggests that it is a site for binding to DNA. The amino-terminal half. containing most of the acidic residues. resembles small, nonhistone proteins and so might have specificity for factors other than DNA. 相似文献
44.
In the western tropical Pacific, the interannual migration of the Indonesian Low convective system causes changes in rainfall that dominate the regional signature of the El Ni?o-Southern Oscillation (ENSO) system. A 96-year oxygen isotope record from a Tarawa Atoll coral (1 degrees N, 172 degrees E) reflects regional convective activity through rainfall-induced salinity changes. This monthly resolution record spans twice the length of the local climatological record and provides a history of ENSO variability comparable in quality with those derived from instrumental climate data. Comparison of this coral record with a historical chronology of EI Ni?o events indicates that climate anomalies in coastal South America are occasionally decoupled from Pacific-wide ENSO extremes. Spectral analysis suggests that the distribution of variance in this record has shifted among annual to interannual periods during the present century, concurrent with observed changes in the strength of the Southern Oscillation. 相似文献
45.
46.
Charmantier A McCleery RH Cole LR Perrins C Kruuk LE Sheldon BC 《Science (New York, N.Y.)》2008,320(5877):800-803
Rapid climate change has been implicated as a cause of evolution in poorly adapted populations. However, phenotypic plasticity provides the potential for organisms to respond rapidly and effectively to environmental change. Using a 47-year population study of the great tit (Parus major) in the United Kingdom, we show that individual adjustment of behavior in response to the environment has enabled the population to track a rapidly changing environment very closely. Individuals were markedly invariant in their response to environmental variation, suggesting that the current response may be fixed in this population. Phenotypic plasticity can thus play a central role in tracking environmental change; understanding the limits of plasticity is an important goal for future research. 相似文献
47.
Identification of the cystic fibrosis gene: chromosome walking and jumping 总被引:253,自引:0,他引:253
J M Rommens M C Iannuzzi B Kerem M L Drumm G Melmer M Dean R Rozmahel J L Cole D Kennedy N Hidaka 《Science (New York, N.Y.)》1989,245(4922):1059-1065
An understanding of the basic defect in the inherited disorder cystic fibrosis requires cloning of the cystic fibrosis gene and definition of its protein product. In the absence of direct functional information, chromosomal map position is a guide for locating the gene. Chromosome walking and jumping and complementary DNA hybridization were used to isolate DNA sequences, encompassing more than 500,000 base pairs, from the cystic fibrosis region on the long arm of human chromosome 7. Several transcribed sequences and conserved segments were identified in this cloned region. One of these corresponds to the cystic fibrosis gene and spans approximately 250,000 base pairs of genomic DNA. 相似文献
48.
Construction of a general human chromosome jumping library, with application to cystic fibrosis 总被引:22,自引:0,他引:22
F S Collins M L Drumm J L Cole W K Lockwood G F Vande Woude M C Iannuzzi 《Science (New York, N.Y.)》1987,235(4792):1046-1049
In many genetic disorders, the responsible gene and its protein product are unknown. The technique known as "reverse genetics," in which chromosomal map positions and genetically linked DNA markers are used to identify and clone such genes, is complicated by the fact that the molecular distances from the closest DNA markers to the gene itself are often too large to traverse by standard cloning techniques. To address this situation, a general human chromosome jumping library was constructed that allows the cloning of DNA sequences approximately 100 kilobases away from any starting point in genomic DNA. As an illustration of its usefulness, this library was searched for a jumping clone, starting at the met oncogene, which is a marker tightly linked to the cystic fibrosis gene that is located on human chromosome 7. Mapping of the new genomic fragment by pulsed field gel electrophoresis confirmed that it resides on chromosome 7 within 240 kilobases downstream of the met gene. The use of chromosome jumping should now be applicable to any genetic locus for which a closely linked DNA marker is available. 相似文献
49.
Radiation carcinogenesis: the sequence of events 总被引:4,自引:0,他引:4
50.
Focal antibody production by transferred spleen cells in irradiated mice 总被引:18,自引:0,他引:18
Lethally irradiated mice were injected with small numbers of normal spleen cells and then immunized with sheep erythrocytes. Antibody activity was found in their spleens in localized areas whose number corresponded to the number of spleen cells injected. When sheep and pig erythrocytes were injected together, antibody against each was found in separate areas. Each area may consist of the progeny of a single precursor cell, restricted to forming a single antibody. 相似文献