Sub-microlitre volumes of normal koala, mouse, dog, rat and cat tears were fractionated using size exclusion-high performance liquid chromatography (SE - HPLC), giving reproducible profiles which were different for each species. Microlitre volumes of tears were also fractionated using sodium dodecylsulphate-polyacrylamide gel electrophoresis (SDS - PAGE), resulting in good separation of individual tear proteins with a species specific distribution. Tears from koalas with conjunctivitis and mice with keratitis were similarly examined and showed mostly quantitative changes. These simple, rapid techniques gave reproducible results and, in contrast to conventional separation techniques, used easily obtainable volumes (as little as 0.75 microl) of tears. Their expansion could allow isola tion, identification and quantitation of individual tear components, enabling effective investigation of changes occurring in disease. 相似文献
Objective: To describe a case of atrioventricular block and syncope secondary to systemic hypertension in a dog with malignant pheochromocytoma. Case Summary: A 13‐year‐old spayed female mixed‐breed dog presented following an acute onset of collapse. The dog was hypertensive and had paradoxical high‐grade second‐degree atrioventricular block with concurrent syncope. Initial emergent therapy included a nitroprusside infusion for the systemic hypertension and vagolytic therapy for the bradyarrhythmia. A left adrenal mass was seen on abdominal ultrasound and was further characterized by MRI. Following medical stabilization with phenoxybenzamine and propantheline, a left adrenalectomy was performed. Histological diagnosis was consistent with a malignant pheochromocytoma. Following tumor removal there has been no further hypertension, atrioventricular block or syncope. Unique information provided: This is the first documented case of a malignant pheochromocytoma presenting as syncope secondary to hypertension induced atrioventricular block. 相似文献
Nitrogen-fixing species can dramatically increase soil acidity and organic matter content, and potentially alter biogeochemical P dynamics. We compared ecosystem P cycling in adjacent stands of N2-fixing red alder (Alnus rubra Bong.) and non-fixing Douglas-fir (Pseudotsuga menziesii Franco) in order to determine whether P-cycling rates within stands were related to soil P forms as measured by sequential P fractionation. Above-ground annual P uptake was 61% greater in the red alder stand, although soil available P, as measured by Bray (NH4F–HCl) extraction, was only 10% of that found in the Douglas-fir stand. Total ecosystem P in the alder stand was only 69% of that found in the Douglas-fir stand, and could indicate a pre-establishment difference between stands. However, the percentage of total soil P released by Bray or NaOH extraction was also lower in the alder stand, which suggests that differences in total P alone did not control the patterns observed in P fractions. Concentrations of inorganic P sorbed to Fe and Al minerals and contained in Fe minerals and apatite were greater under Douglas-fir, while organic P was slightly greater under red alder. While fluxes of P in litterfall, uptake and resorption were 94, 60 and 292% higher in the alder stand, soil extractable fractions meant to represent available P were lower under alder. Static measures of available P do not appear to adequately reflect P supply, and the development of techniques to assess P turnover is needed to better understand cycling and plant availability of P. 相似文献
The response of a forest to nutrient and moisture stresses is reflected in nutritional, physiological, and structural changes that include efficiency of nutrient use, translocation and cycling of nutrients, transpiration, retention of foliage, below-ground and above-ground allocation of carbon, as well as the structural development of the forest stand and its growth characteristics. This article reviews the relationship of forest ecosystems to nutrient and moisture stresses and addresses the means by which productivity can be enhanced by altering nutrient and moisture regimes.
Considerable research has focused on optimizing productivity by minimizing nutrient and moisture stresses. Research involved in nutrient additions has led to the use of commercial fertilizers to improve forest productivity. The results suggest that many forests are deficient in N and P and, to a lesser extent, S, K, Mg and trace elements. The duration of response for most nutrient additions is, however, relatively brief and the efficiency of the tree in using fertilizer is relatively poor. Long-term correction of nutrient deficiencies is seldom achieved with chemical fertilizers. However, N added through symbiotic fixation or, on a more limited scale, through addition of municipal and industrial waste by-products, can provide an excellent long-term growth response.
It is seldom feasible to change the moisture regime of a forest ecosystem through irrigation. However, field trials involving irrigation have demonstrated that moisture stress can limit productivity. There are various ways of minimizing moisture stress without irrigation, including mulching, removing ground-cover vegetation, and changing the spatial characteristics of the forest cover.
Research trials show that forest ecosystems will respond to moisture and nutrient additions; however, these responses and interactions between nutrients and moisture are typically poorly understood, and many questions remain unanswered: Does fertilization increase moisture-use efficiency of a forest or simply improve the nutrition of the site? Does improving the moisture regime of a site improve productivity primarily by decreasing moisture stress or by increasing nutrient availability and the rate of nutrient uptake? Is there a synergism in growth response with the addition of both nutrients and moisture? The linkages between nutrients and moisture appear inseparable and confound experimentation in this field. Answers to these questions and issues need to be found for the future development of plantation forestry. 相似文献
Agroforestry Systems - Peach palm (Bactris gasipaes) was domesticated as a fruit crop by the first Amazonians in traditional Neotropical agroforestry systems, but research and development... 相似文献