全文获取类型
收费全文 | 873篇 |
免费 | 69篇 |
国内免费 | 2篇 |
专业分类
林业 | 16篇 |
农学 | 23篇 |
基础科学 | 3篇 |
53篇 | |
综合类 | 151篇 |
农作物 | 24篇 |
水产渔业 | 36篇 |
畜牧兽医 | 602篇 |
园艺 | 6篇 |
植物保护 | 30篇 |
出版年
2023年 | 9篇 |
2021年 | 8篇 |
2020年 | 14篇 |
2019年 | 15篇 |
2018年 | 21篇 |
2017年 | 12篇 |
2016年 | 17篇 |
2015年 | 8篇 |
2014年 | 22篇 |
2013年 | 48篇 |
2012年 | 17篇 |
2011年 | 27篇 |
2010年 | 31篇 |
2009年 | 31篇 |
2008年 | 19篇 |
2007年 | 38篇 |
2006年 | 41篇 |
2005年 | 37篇 |
2004年 | 27篇 |
2003年 | 25篇 |
2002年 | 28篇 |
2001年 | 25篇 |
2000年 | 22篇 |
1999年 | 22篇 |
1998年 | 30篇 |
1997年 | 19篇 |
1996年 | 18篇 |
1995年 | 19篇 |
1994年 | 6篇 |
1993年 | 9篇 |
1992年 | 26篇 |
1991年 | 16篇 |
1990年 | 14篇 |
1989年 | 18篇 |
1988年 | 15篇 |
1987年 | 14篇 |
1986年 | 7篇 |
1985年 | 14篇 |
1984年 | 5篇 |
1983年 | 5篇 |
1980年 | 5篇 |
1979年 | 9篇 |
1977年 | 9篇 |
1976年 | 6篇 |
1973年 | 6篇 |
1971年 | 10篇 |
1970年 | 4篇 |
1969年 | 5篇 |
1914年 | 4篇 |
1902年 | 4篇 |
排序方式: 共有944条查询结果,搜索用时 15 毫秒
901.
Bettschart-Wolfensberger R Freeman SL Jäggin-Schmucker N Clarke KW 《American journal of veterinary research》2001,62(4):500-507
OBJECTIVE: To determine the minimal infusion rate of propofol in combination with medetomidine for long-term anesthesia in ponies and the effects of atipamezole on recovery. ANIMALS: 12 ponies. PROCEDURE: Ponies were sedated with medetomidine (7 microg/kg of body weight, IV). Ten minutes later, anesthesia was induced with propofol (2 mg/kg, IV). Anesthesia was maintained for 4 hours, using an infusion of medetomidine (3.5 microg/kg per hour, IV) and propofol at a rate sufficient to prevent ponies from moving after electrical stimulation. Arterial blood pressures and blood gas analysis, heart rates, and respiratory rates were monitored. For recovery, 6 ponies were given atipamezole (60 microg/kg, IV). Induction and recovery were scored. RESULTS: Minimal propofol infusion rates ranged from 0.06 to 0.1 mg/kg per min. Mean arterial blood pressure was stable (range, 74 to 86 mm Hg), and heart rate (34 to 51 beats/min) had minimal variations. Variable breathing patterns were observed. Mean PaO2 (range, 116 to 146 mm Hg) and mean PaCO2 (range, 48 to 51 mm Hg) did not change significantly with time, but hypoxemia was evident in some ponies (minimal PaO2, 47 mm Hg). Recovery was fast and uneventful with and without atipamezole (completed in 20.2 and 20.9 minutes, respectively). CONCLUSIONS AND CLINICAL RELEVANCE: Infusion of a combination of medetomidine and propofol was suitable for prolonged anesthesia in ponies. Recovery was rapid and uneventful. A combination of propofol and medetomidine may prove suitable for long-term anesthesia in horses. Monitoring of blood gases is essential because of potential hypoxemia. 相似文献
902.
Effects of Mannheimia haemolytica leukotoxin on apoptosis and oncosis of bovine neutrophils 总被引:1,自引:0,他引:1
Cudd LA Ownby CL Clarke CR Sun Y Clinkenbeard KD 《American journal of veterinary research》2001,62(1):136-141
OBJECTIVE: To investigate the concentration-dependent effects of Mannheimia haemolytica (formerly Pasteurella haemolytica) leukotoxin (LKT) on apoptosis and oncosis in bovine neutrophils and to examine the role of calcium ions (Ca2+) in LKT-induced apoptosis. SAMPLE POPULATION: Neutrophils isolated from blood samples obtained from healthy calves. PROCEDURE: Neutrophil suspensions were exposed to lytic or sublytic dilutions of LKT and then examined by use of transmission electron microscopy (TEM) or gel electrophoresis. Contribution of extracellular Ca2+ to LKT-induced apoptosis was investigated by incubating neutrophils with LKT or control solutions in buffer containing 1 mM CaCl2 or in Ca2+-free buffer containing 1 mM ethylene glycol-bis (b-aminoethyl ether)-N,N-tetraacetic acid (EGTA) prior to diphenyl amine analysis. RESULTS: Examination by TEM revealed that bovine neutrophils exposed to lytic dilutions of LKT had changes consistent with oncosis, whereas neutrophils exposed to sublytic dilutions of LKT and staurosporin, an inducer of apoptosis, had changes consistent with apoptosis. Effects of sublytic dilutions of LKT on apoptosis were confirmed by gel electrophoresis. Replacement of extracellular Ca2+ with EGTA, a Ca2+ chelator, reduced apoptosis attributable to the calcium ionophore A23187, but it did not have significant effects on apoptosis induced by LKT or staurosporin. CONCLUSIONS AND CLINICAL RELEVANCE: The ability of LKT to cause apoptosis instead of oncosis is concentration-dependent, suggesting that both processes of cell death contribute to an ineffective host-defense response, depending on the LKT concentration in pneumonic lesions. Furthermore, although Ca2+ promotes A23187-induced apoptosis, it is apparently not an essential second messenger for LKT-induced apoptosis. 相似文献
903.
Canine parvovirus (CPV) has been reported throughout the world since the late 1970s. Published information was reviewed to draw insights into the epidemiology, pathogenesis, diagnosis, treatment and outcomes of CPV disease in Australia and the role of scientific research on CPV occurrence, with key research discoveries and knowledge gaps identified. Australian researchers contributed substantially to early findings, including the first reported cases of parvoviral myocarditis, investigations into disease aetiopathogenesis, host and environmental risk factors and links between CPV and feline panleukopenia. Two of the world's first CPV serological surveys were conducted in Australia and a 1980 national veterinary survey of Australian and New Zealand dogs revealed 6824 suspected CPV cases and 1058 deaths. In 2010, an Australian national disease surveillance system was launched; 4940 CPV cases were reported between 2009 and 2014, although underreporting was likely. A 2017 study estimated national incidence to be 4.12 cases per 1000 dogs, and an annual case load of 20,110 based on 4219 CPV case reports in a survey of all Australian veterinary clinics, with a 23.5% response rate. CPV disease risk factors identified included socioeconomic disadvantage, geographical location (rural/remote), season (summer) and rainfall (recent rain and longer dry periods both increasing risk). Age <16 weeks was identified as a risk factor for vaccination failure. Important knowledge gaps exist regarding national canine and feline demographic and CPV case data, vaccination coverage and population immunity, CPV transmission between owned dogs and other carnivore populations in Australia and the most effective methods to control epizootics. 相似文献
904.
Spectral and thermal sensing for nitrogen and water status in rainfed and irrigated wheat environments 总被引:1,自引:1,他引:1
G. J. Fitzgerald D. Rodriguez L. K. Christensen R. Belford V. O. Sadras T. R. Clarke 《Precision Agriculture》2006,7(4):233-248
Variable-rate technologies and site-specific crop nutrient management require real-time spatial information about the potential for response to in-season crop management interventions. Thermal and spectral properties of canopies can provide relevant information for non-destructive measurement of crop water and nitrogen stresses. In previous studies, foliage temperature was successfully estimated from canopy-scale (mixed foliage and soil) temperatures and the multispectral Canopy Chlorophyll Content Index (CCCI) was effective in measuring canopy-scale N status in rainfed wheat (Triticum aestivum L.) systems in Horsham, Victoria, Australia. In the present study, results showed that under irrigated wheat systems in Maricopa, Arizona, USA, the theoretical derivation of foliage temperature unmixing produced relationships similar to those in Horsham. Derivation of the CCCI led to an r
2 relationship with chlorophyll a of 0.53 after Zadoks stage 43. This was later than the relationship (r
2 = 0.68) developed for Horsham after Zadoks stage 33 but early enough to be used for potential mid-season N fertilizer recommendations. Additionally, ground-based hyperspectral data estimated plant N (g kg−1) in Horsham with an r
2 = 0.86 but was confounded by water supply and N interactions. By combining canopy thermal and spectral properties, varying water and N status can potentially be identified eventually permitting targeted N applications to those parts of a field where N can be used most efficiently by the crop. 相似文献
905.
Although ordinary chondrite (OC) meteorites dominate observed falls, the identification of near-Earth and main-belt asteroid sources has remained elusive. Telescopic measurements of 35 near-Earth asteroids ( approximately3 kilometers in diameter) revealed six that have visible wavelength spectra similar to laboratory spectra of OC meteorites. Near-Earth asteroids were found to have spectral properties that span the range between the previously separated domains of OC meteorites and the most common (S class) asteroids, suggesting a link. This range of spectral properties could arise through a diversity of mineralogies and regolith particle sizes, as well as through a time-dependent surface weathering process. 相似文献
906.
Wysession ME Langenhorst A Fouch MJ Fischer KM Al-Eqabi GI Shore PJ Clarke TJ 《Science (New York, N.Y.)》1999,284(5411):120-125
Observations of core-diffracted P (Pdiff) and SH (SHdiff) waves recorded by the Missouri-to-Massachusetts (MOMA) seismic array show that the ratio of compressional (P) seismic velocities to horizontal shear (SH) velocities at the base of the mantle changes abruptly from beneath the mid-Pacific (VP/VS = 1.88, also the value predicted by reference Earth models) to beneath Alaska (VP/VS = 1.83). This change signifies a sudden lateral variation in material properties that may have a mineralogical or textural origin. A textural change could be a result of shear stresses induced during the arrival at the core of ancient lithosphere from the northern Pacific paleotrench. 相似文献
907.
908.
909.
910.
Clarissa MR. Moreira Renato LS. Oliveira Gilberto A. Costa Katia B. Corgozinho Stelio PL. Luna Heloisa JM. Souza 《Veterinary anaesthesia and analgesia》2021,48(1):134-141
ObjectiveTo evaluate the analgesic efficacy and safety of tumescent local anesthesia (TLA) in cats undergoing unilateral mastectomy.Study designProspective clinical trial.AnimalsA total of 12 ovariohysterectomized female cats.MethodsAll animals were premedicated with pethidine (4 mg kg–1) intramuscularly (IM), followed by induction of anesthesia with propofol (5 mg kg–1) intravenously and maintenance with isoflurane in oxygen. A refrigerated TLA solution (15 mL kg–1, 8 °C) was injected using a Klein cannula. The solution was composed of 0.5 mL of epinephrine (1 mg mL–1) and 40 mL of 2% lidocaine added to 210 mL lactated Ringer’s solution (final lidocaine concentration 0.32%). Heart and respiratory rates, systolic arterial blood pressure, temperature and oxygen saturation were measured during anesthesia. Blood samples were collected from the jugular vein for measurement of plasma lidocaine concentration using high performance liquid chromatography. Postoperative pain scores were evaluated hourly for 6 hours. Analgesic rescue was performed with tramadol (2 mg kg–1) IM and meloxicam (0.15 mg kg–1) subcutaneously.ResultsPlasma lidocaine concentration peaked at 90 minutes after injection of TLA, but no concentration considered toxic for the species was measured. The median postoperative analgesia time was 6 hours after injection of TLA.ConclusionsThis study found that TLA prevented sympathetic response to noxious stimuli during anesthesia and provided satisfactory postoperative analgesia in cats submitted to total unilateral mastectomy, with no apparent signs of toxicity.Clinical relevanceTLA can prevent sympathetic stimulation resulting from noxious stimuli during anesthesia, promoting good intraoperative conditions, proving to be a viable addition to analgesia in cats submitted to a total unilateral mastectomy. 相似文献