Protection against respiratory infection with bovine virus diarrhoea virus by passively acquired antibody

Susceptibility to infection with bovine virus diarrhoea virus (BVDV) was compared for calves with varying amounts of specific antibody in their sera passively acquired from the ingestion of colostrum. Challenge consisted of intranasal exposure to a strain of BVDV isolated from an outbreak of respiratory disease. Resistance to infection, as judged by nasopharyngeal shedding of virus, was directly related to the titre of neutralizing antibodies in sera. Besides protecting against infection of the upper respiratory tract, passive antibody, which was mainly IgG1, also protected against viraemia and, to a lesser extent, leukopenia. In the presence of colostral antibody, neutralizing and IgG1 antibody responses were apparently inhibited, but a specific IgG2 response occurred.     

The isolation of multiple strains of Mycoplasma ovipneumoniae from individual pneumonic sheep lungs.

The heterogeneity of Mycoplasma ovipneumoniae isolates from the lungs of sheep with chronic non-progressive pneumonia (CNP) from the same flock raised the possibility that multiple isolates derived from one lung were not all identical. To test this hypothesis, thirty isolates were obtained from each of six pneumonic sheep lungs at slaughter. Four lungs had relatively severe lesions and from each of these, three or four strains of M. ovipneumonia, distinguishable by REA and in most cases by SDS-PAGE, were detected. From the lungs of each of two sheep with mild lesions, two strains of M. ovipneumoniae were detected. Four isolates from one lung were further examined by restriction endonuclease analysis (REA) using many restriction endonucleases. Those which differed with EcoRI also differed when other restriction endonucleases were used. However, partial digests occurred mainly with those restriction endonucleases which recognise cytosine-rich sequences. The presence of multiple strains of one species of microorganism in individual lesions is an unusual concept which may not be limited to one disease or to one host.     

Vaccination of calves with a diaminopimelic acid mutant of Salmonella typhimurium.

The purpose of this study was to evaluate the safety and efficacy of a diaminopimelic acid mutant of Salmonella typhimurium as a vaccine for calves. Transposon techniques were used to create a stable nonreverting diaminopimelic acid mutant of a virulent S. typhimurium strain. Calves were vaccinated at weekly intervals with the diaminopimelic acid mutant given as an oral dose of 10(10) organisms, followed by two subcutaneous doses of 10(9) organisms. The calves tolerated vaccination well and the vaccine strain was eliminated from the feces within four days. Of five vaccinated calves, three survived challenge with 5 X 10(9) organisms of the parent strain whereas all five unvaccinated calves that were challenged died. The surviving calves eliminated the challenge organism from the feces within three weeks.     

The effects of induced parturition on the incidence of clinical disease and mortality in dairy cows from commercial herds in south-western Victoria

SUMMARY The effects of induced parturition on clinical disease and mortality of cows were studied in 51 winter-calving, pasture-fed, dairy herds in south-western Victoria. Parturition was induced when most cows were between 27 and 35 weeks of pregnancy. Control groups were selected from herdmates that were not induced to calve. The incidence of retained placenta was much greater among induced cows of all ages. The proportion of cows affected by any clinical disease other than retained placenta, and the incidence of mortality were much greater after induction among cows aged over 6 years but not among cows aged 3 to 6 years.     

胚胎移植技术的应用及其发展

动物胚胎移植首次成功(Heape 1891)距今已近一百年了。在这期间,又有多种动物胚胎移植试验成功,技术也不断改进。但是,进展较大的还是近二十年间。1981年Betterid-ge总结了胚胎移植历史,列出了13种动物胚胎移植首次成功的时间和试验者[兔1891Heape;大鼠1933 Nicholas;绵羊1934Warwick等;山羊(自身重移植)1934Warwick等;小鼠1942 Fekete＆Lit-tle;奶牛(流产)1949 Umbaugh;山羊1949 Warwick＆Berry;奶牛1951Willett等;猪1951 Kvasnickii;奶牛     

Effects of cryotherapy on the palmar and plantar digital nerves in the horse

The duration of anesthetic effect and the histopathologic changes resulting from a controlled freeze of the palmar and plantar digital nerves in the horse were evaluated. Two techniques were compared: (i) nerves were frozen by direct application of the cryoprobe after surgical exposure and (ii) nerves were frozen by percutaneous application of the cryoprobe to the overlying skin. Return of skin sensation and ability to detect a stimulus were used to determine return of nerve function. The duration of anesthetic effect was significantly (P less than 0.005) longer for nerves frozen after surgical exposure than for those frozen by the percutaneous technique (mean 156 days vs 47.5 days). At the termination of the study, horses were euthanatized and all nerves were examined histologically. Neuromas-in-continuity were observed in 10 of 28 frozen nerves. Classification was based on the involvement of the supporting fibrous connective tissues of the nerve, endoneurium, perineurium, and epineurium. The direct technique of freezing the nerve after surgical exposure was repeatable and could be used to provide temporary neurectomy in the horse. The percutaneous technique caused a temporary loss of pain perception, but could not be relied on to interrupt nerve function for longer than a few weeks.     

Effect of coccidiosis on reproductive maturation of male Japanese quail

The effects of coccidiosis on reproductive development of male Japanese quail were examined. Male Japanese quail were exposed to high (5 x 10(5) sporulated oocysts/quail) or low (5 x 10(3) sporulated oocysts/quail) doses of Eimeria uzura at 16 or 30 days of age and sampled at 37 days. Quail given high doses of coccidia had reduced testes weight and lowered circulating concentrations of androgen compared with control males. Low doses of coccidia did not affect testes weight but did result in elevated plasma androgen levels. There were no differences in average testes weights by 51 days; however, plasma androgen was still reduced in most groups. To study the effects of coccidiosis on egg production, males exposed to high doses of coccidia at 16 (16H) or 30 (30H) days of age were mated with control females, and control males were mated with control or 16H females. The onset of laying was delayed for 5 days in the control male: 16H female group. During the first week of production, eggs from females bred to 30H males had lower fertility and hatchability than those bred to control or 16H males. By the third week of production, levels of fertility were similar. Apparently, exposure of quails to coccidiosis before sexual maturation might result in long-term effects on later reproductive capability.     

The seroprevalence of verocytotoxin-producing Escherichia coli in Ontario dairy cows and associations with production and management

The prevalences of neutralizing antibodies to verocytotoxins (VT) from four reference strains of VT-producing Escherichia coli were determined in sera from 306 dairy cows from 164 randomly selected herds in southern Ontario, Canada. Prevalences (and 95% confidence intervals) of antibodies capable of neutralizing VTs from the following reference strains of E. coli were: Strain H30 (VT1), 78.4% (73.7%–83.1%); Strain 32511 (VT2, VT2v), 1.3% (0–2.9%); Strain 933W (VT2), 5.6% (3.0%–8.2%); Strain K12B (VTe), 12.1% (8.5%–15.7%). Thirty-nine sera contained neutralising antibodies to more than one type of VT. Thus, neutralising antibodies to verocytotoxins were common among dairy cows in Ontario, Canada.

At the individual-cow level, increased risk of VT1 antibodies was observed in association with an increase in age and in Holstein-Friesian cows compared with other breeds. The herd-level proportion of VT1-positive cows was reduced in association with an increase in herd size, storage of manure in an open pile, and occasional use of equipment to handle both manure and feed.