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C. W. Tice J. T. Rosa A. E. Wilkinson E. A. Stokdyk E. L. Newdick Verdal T. Maxwell C. F. Clark W. Stuart Charles A. Thompson J. Gilbert Borton Ellwood Douglass Orley G. Bowen Wm. H. Martin J. Norton Woodruff John J. Longbon W. H. Zimmerly J. G. Milward 《American Journal of Potato Research》1924,1(9):166-178
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W. Stuart W. M. Peacock W. S. Baird W. Saxby Blair R. H. Helmer W. H. Hicks W. R. Leslie B. F. Lutman H. H. Mann S. D. Nagpurkar G. S. Kulkarni R. S. Kasargode S. R. Paranjupe B. M. Joshi J. A. McClary W. C. Mckillican Wm. H. Martin A. M. Musser C. A. Ludwig C. F. Clark R. G. Newton T. H. Parks E. E. Clayton F. H. Reed R. N. Salaman E. M. Straight C. T. Tice W. C. Edmundson M. J. Tinline H. O. WERNER R. F. Howard C. F. Clark C. M. White T. K. Wolfe 《American Journal of Potato Research》1924,1(4):54-66
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C. Tice H. G. Zuckerman W. C. Edmundson W. L. Floyd G. W. Waller A. J. Johnston E. P. Miller E. A. Stokdyk G. L. Tiebout H. C. Moore Walter M. Peacock Andrew Schnetzler Senator J. Gilbert Borton E. V. Hardenburg J. Tucker George H. Valentine A. G. Tolaas P. N. Davis H. L. Bolley W. K. McCullock Walter M. Peacock C. F. Clark 《American Journal of Potato Research》1925,2(2):65-82
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J Yang A K Dunker J R Powers S Clark B G Swanson 《Journal of agricultural and food chemistry》2001,49(7):3236-3243
Beta-lactoglobulin (beta-LG) was treated with high hydrostatic pressure (HHP) at 600 MPa and 50 degrees C for selected times as long as 64 min. The intrinsic tryptophan fluorescence of beta-LG indicated that HHP treatment conditions induced a conformational change. HHP treatment conditions also promote a 3-fold increase in the extrinsic fluorescence of 1-anilinonaphthalene-8-sulfonate and a 2.6-fold decrease for cis-paraneric acid, suggesting an increase in accessible aromatic hydrophobicity and a decrease in aliphatic hydrophobicity. Far-ultraviolet circular dichroism (CD) spectra reveal that the secondary structure of beta-LG converts from native beta-sheets to non-native alpha-helices following HHP treatment, whereas near-ultraviolet CD spectra reveal that the native tertiary structure of beta-LG essentially disappears. Urea titrations reveal that native beta-LG unfolds cooperatively, but the pressure-treated molecule unfolds noncooperatively. The noncooperative state is stable for 3 months at 5 degrees C. The nonaccessible free thiol group of cysteine121 in native beta-LG became reactive to Ellman's reagent after adequate HHP treatment. Gel electrophoresis with and without beta-mercaptoethanol provided evidence that the exposed thiol group was lost concomitant with the formation of S-S-linked beta-LG dimers. Overall, these results suggest that HHP treatments induce beta-LG into hydrophobic molten globule structures that remain stable for at least 3 months. 相似文献
1000.
RA Elgawish Y Ogata T Hidaka T Nii Y Yoshimura N Isobe 《Reproduction in domestic animals》2018,53(4):1013-1015
This study was carried out to examine the changes in plasma concentrations of the Ca‐binding antimicrobial proteins S100A7 and S100A8 during pregnancy in dairy cows. Holstein Friesian cows (n = 19) were inseminated with Holstein Friesian semen. Blood was collected at days 30, 60, 90, 120, 150, 180, 210, 240 and 270 after insemination. Plasma was used for measuring the concentrations of S100A7 and S100A8. Both S100A7 and S100A8 concentrations showed similar patterns during gestation; they increased during the midgestation, between days 90 and 180, and then declined before calving. The findings indicated that plasma concentrations of S100A7 and S100A8 did not change significantly during pregnancy in cows. Further studies are required to determine the roles of S100A7 and S100A8 in physiological function during pregnancy in dairy cows. 相似文献