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91.
Manipulations of Shrimp Embryos: A Viable Technique for the Removal of the Hatching Envelope 总被引:1,自引:0,他引:1
John W. Lynn Patricia S. Glas Philip L. Hertzler Wallis H. Clark Jr. Jeffrdy D. Green 《Journal of the World Aquaculture Society》1993,24(1):1-5
The hatching envelope elevated around the spawned eggs of penaeoidean eggs presents a formidable barrier for manipulation of the embryo. By spawning eggs into seawater containing 3-amino-1,2,4-triazole and subsequently passing the eggs through Nitex screening approximately 45 min after spawning, the hatching envelope can be effectively removed with little or no damage to the embryos. The time and extent to which the envelope is removed can be varied. Embryos treated in this manner continue to develop and the method provides a new technique with which investigators can gain access to the surface of the egg or embryo. 相似文献
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J S Lumsden P Clark S Hawthorn M Minamikawa S G Fenwick M Haycock & B Wybourne 《Journal of fish diseases》2002,25(3):155-163
A syndrome affecting cultured chinook salmon, Oncorhynchus tshawytscha (Walbaum), characterized by distended abdomens, gastric dilation, air sacculitis (GDAS), increased feed conversion rates and increased mortality has been recognized in New Zealand. Affected fish were most obvious in sea cages but were also present in fresh water. Mortality rates associated with this condition were highest in late summer and approached 6% per month. A dilated and flaccid stomach, without visible rugal folds containing copious oil, watery fluid or undigested feed was typical. Gastric mucosal ulceration or inflammation were not present. The air sacculitis consisted of a thickened, dilated bladder with a mixed mucosal inflammatory infiltrate and a luminal exudate associated with large numbers of morphologically diverse bacteria. Gastric dilation or air sacculitis occurred alone or together in the same fish. In a group of 20 subclinically affected fish with or without gastric dilation, there were no significant differences in weight, length, serum osmolality, sodium, total protein or packed cell volume. Twenty-three severely affected fish had significantly ( P < 0.05) higher serum osmolality but similar sodium and total protein to that of clinically normal fish. 相似文献
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Elemental Speciation and Distribution in Sediments of a Eutrophied Subtropical Freshwater Reservoir Using Postextraction Normalisation 总被引:1,自引:0,他引:1
Darren J. Akhurst Malcolm W. Clark Amanda J. Reichelt-Brushett Graham B. Jones 《Water, air, and soil pollution》2012,223(7):4589-4604
The speciation and distribution of trace and major elements (Al, As, Cd, Cr, Cu, Fe, Mn, Ni, P, Pb and Zn) in the sediments of Emigrant Creek Dam (ECD), New South Wales Australia were investigated using sequential extraction, postextraction normalisation and spatial mapping to indicate source and dispersion patterns. Subsurface coring provided an estimate of elemental enrichment and showed that As 1.9?>?P 1.7?>?N 1.5????Cd 1.5?>?Mn 1.3 were enriched. Moreover, a high proportion of the enriched elements (mean 57, 34, 47 and 87?% for As, P, Cd and Mn, respectively) were assessed as being bioavailable. Comparisons with ISQGs found that sediments from sites in proximity to Emigrant Creek inflows had the highest accumulations of metals and the greatest potential for causing biological harm. Spatially, contaminants accumulate in ECD sediments adjacent to anthropogenic sources including a cattle dip site, dredged sediment and macrophyte dump areas, and agricultural/residential runoff. Moreover, the integrated technique and postextraction normalisation allow assessment of texturally diverse and difficult sediments. 相似文献
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Elizabeth B Steeves Brian J Chelack Edward G Clark Deborah M Haines 《Journal of veterinary diagnostic investigation》2002,14(1):53-56
In this study, 50 cases of canine pemphigus foliaceus and 49 cases of canine superficial pyoderma were examined by immunohistochemical staining for patterns of desmoglein expression. In 31/50 (62%) of pemphigus foliaceus cases, there was an altered staining pattern for desmoglein consisting of distinct clumped deposits at the periphery of keratinocytes and/or dark cytoplasmic staining of acantholytic cells (consistent with internalization of desmoglein). In contrast, desmoglein staining in biopsies from cases of superficial pyoderma was diffusely pale without evidence for clumping or distinct internalization. This study demonstrates that epidermal desmoglein expression is altered in some cases of pemphigus foliaceus in dogs and suggests that immunohistochemical staining for this protein may be useful in diagnosis. 相似文献