微量Bradford法测定提纯禽结核菌素蛋白含量

研究用96孔微量板Bradford法测定提纯禽结核菌素蛋白含量。通过不同浓度的待测禽结核菌素与考马斯亮蓝G-250溶液混合后，在595nm光波下测定各孔样品的OD值。微量Bradford法检测禽结核菌素的线性范围是50—1000μg／mL，相关系数为0．999，最低定量限为50μg／mL。试验用3支禽结核菌素国际参照品，分别配制成125、250、500μg／mL三种不同浓度，每支每种浓度测定4次，计算实际测得的蛋白质浓度。结果显示，批内相对标准偏差为1．6％～4．4％；批间相对标准偏差0．7％-2．2％。研究结果表明微量Bradford法可作为测定禽结核菌素蛋白含量的一种方法。     

Perceptions and priorities of owners of dogs with heart disease regarding quality versus quantity of life for their pets

OBJECTIVE: To describe the relative importance of pets' quality versus quantity of life among owners of dogs with heart disease. DESIGN: Prospective questionnaire-based clinical study. SAMPLE POPULATION: Owners of 201 dogs with heart disease. PROCEDURES: Owners each completed a questionnaire that was designed to ascertain the relative importance of quality versus quantity of life for their pet and to assess the owners' willingness to trade survival time for quality of life, if that were possible. Analyses were performed to evaluate factors associated with owner willingness to trade time for quality of life. RESULTS: Most owners (170/197 [86%]) were willing to trade survival time for quality of life for their heart disease-affected dogs; of those owners, 88 (52%) were willing to trade 6 months. Owners were highly concerned with detection of perceived pet suffering and their pet's ability to interact with them. Owners whose pets had respiratory difficulty or fainting episodes and were treated on an outpatient basis had a greater willingness to trade survival time than owners of dogs that were treated on an emergency basis. Among owners willing to trade time for quality of life, younger owners and those whose pets had fainting episodes were willing to trade the most amount of time. CONCLUSIONS AND CLINICAL RELEVANCE: Results indicated that quality of life is highly important to owners of dogs with heart disease. Owners' priorities partly depend on owner age and the pet's clinical circumstances; ongoing client-veterinarian communication is important to optimize treatment success as perceived by owners.     

Shuttle imaging radar experiment

The shuttle imaging radar (SIR-A) acquired images of a variety of the earth's geologic areas covering about 10 million square kilometers. Structural and geomorphic features such as faults, folds, outcrops, and dunes are clearly visible in both tropical and arid regions. The combination of SIR-A and Seasat images provides additional information about the surface physical properties: topography and roughness. Ocean features were also observed, including large internal waves in the Andaman Sea.     

Dynamics of Oxidation of a Fe2+-Bearing Aluminosilicate (Basaltic) Melt

Rutherford backscattering spectroscopy (RBS) and microscopy demonstrate that the approximately 1400°C oxidation of levitated droplets of a natural Fe2+-bearing aluminosilicate (basalt) melt occurs by chemical diffusion of Fe2+ and Ca2+ to the free surface of the droplet; internal oxidation of the melt results from the required counterflux of electron holes. Diffusion of an oxygen species is not required. Oxidation causes the droplets to go subsolidus; magnetite (Fe3O4) forms at the oxidation-solidification front with a morphology suggestive of a Liesegang-band nucleation process.     

Bovine-associated MRSA ST398 in The Netherlands

During routinely screening (50.000 milk samples on an annual basis) 14 MRSA ST398 strains were identified in the period of January 2008 to September 2008 in 14 different dairy herds located in the provinces Overijssel and Gelderland, The Netherlands. Molecular analysis was performed by Cfr9I PFGE, ST398-specific diagnostic PCR, spa typing, SCCmec typing and Panton-Valentine Leukocidin (PVL) gene PCR. The molecular analyses of 14 MRSA (one MRSA strain per herd) strains revealed that all strains belong to ST398 with 3 closely related spa types (t011, t108 and t889, all commonly found in pigs) and carry 2 different SCCmec types, IVa and V. All MRSA strains were resistant to two or more classes of antibiotics and also PVL negative. The majority of farms (n = 9, 64%) harboured combined livestock with both cows and pigs present. Our study contributes to the growing evidence that MRSA ST398 is transmitted among various animal species and can be considered as an etiological agent of mastitis in dairy cows.     

Overview of the shuttle imaging radar-B preliminary scientific results

The Shuttle Imaging Radar-B experiment consisted of a large number of scientific investigations in the earth sciences. Nine oceanographic experiments were conducted to study the generation and propagation of surface waves, the dynamics of internal waves, oil slick detection, and the properties of southern polar ice. Stereo imaging from space allowed three-dimensional viewing of surface features. Geologic experiments were conducted to study subsurface penetration, structural mapping, and lithologic classification. Imaging radar angular scatterometry was used in the vegetation cover, forest type, and urban areas classification experiments. This article provides an overview of the scientific results, some of which are also presented in this issue.     

Unusual ovarian activity in a mare preceding the development of an ovarian granulosa cell tumour

An 8-year-old mare, with a foal at foot, was inseminated on foal heat with frozen semen, with the resultant pregnancy lost between days 34 and 41. The right ovary developed a large anovulatory follicle that was non-responsive to multiple doses of ovulating agents. The follicle eventually appeared to luteinise, although plasma progesterone concentrations did not reflect this. Another follicle developed, responded to GnRH and resulted in a pregnancy from frozen semen that went to term with a healthy foal. When the mare was examined after foaling, the structure on the right ovary appeared to be a granulosa cell tumour; the left ovary was smaller than normal and non-functional. Surgical removal of the right ovary before increasing photoperiod resulted in a return to function of the left ovary and a pregnancy to frozen semen on the second cycle following removal. Figures showing concentrations of inhibin, progesterone, androstenedione, oestradiol and testosterone are presented for this entire period. Unusual ovarian activity in the mare might be a prelude to the development of a granulosa cell tumour.     

Disseminated Mycobacterium avium complex infection following renal transplantation in a cat

An 11-year-old cat with a history of renal transplantation and treatment with cyclosporine and prednisolone was examined because of vomiting, diarrhea, inappetence, lethargy, and weight loss. Abdominal ultrasonography revealed 2 large heteroechoic masses thought to be mesenteric lymph nodes. Ultrasound-guided biopsy was performed, and histologic examination of biopsy specimens revealed granulomatous inflammation of presumptive lymph node tissue. Examination of sections stained with acid-fast stains revealed innumerable acid-fast bacilli within histiocytes, and a presumptive diagnosis of mycobacteriosis was made. The cat's clinical condition deteriorated, and euthanasia was elected. At necropsy, granulomatous inflammation was present within the mesenteric lymph nodes, spleen, liver, small and large intestines, lungs, and bone marrow. Bacterial culture yielded Mycobacterium avium, a slow-growing, opportunistic, saprophytic mycobacterium that can cause tuberculous lesions that are clinically indistinguishable from those associated with classic tuberculosis. It is a rare cause of disseminated mycobacteriosis in human transplant recipients. To our knowledge, this is the first report of disseminated M avium complex infection in a feline transplant recipient.